Overview of Affinity Tags for Protein Purification

2004 ◽  
Vol 36 (1) ◽  
Author(s):  
Michelle E. Kimple ◽  
John Sondek
Keyword(s):  
Protein Purification ◽  
Affinity Tags

Affinity Tags for Protein Purification

2020 ◽  
Vol 21 (8) ◽  
pp. 821-830
Author(s):  
Vibhor Mishra
Keyword(s):  
Protein Purification ◽  
Protein Complexes ◽  
Affinity Purification ◽  
Protein Domain ◽  
Affinity Tag ◽  
Small Peptides ◽  
Affinity Tags ◽  
C Terminus ◽  
Solubility Enhancers ◽  
As Solubility

The affinity tags are unique proteins/peptides that are attached at the N- or C-terminus of the recombinant proteins. These tags help in protein purification. Additionally, some affinity tags also serve a dual purpose as solubility enhancers for challenging protein targets. By applying a combinatorial approach, carefully chosen affinity tags designed in tandem have proven to be very successful in the purification of single proteins or multi-protein complexes. In this mini-review, the key features of the most commonly used affinity tags are discussed. The affinity tags have been classified into two significant categories, epitope tags, and protein/domain tags. The epitope tags are generally small peptides with high affinity towards a chromatography resin. The protein/domain tags often perform double duty as solubility enhancers as well as aid in affinity purification. Finally, protease-based affinity tag removal strategies after purification are discussed.

Comparison of affinity tags for protein purification

2005 ◽  
Vol 41 (1) ◽  
pp. 98-105 ◽  
Author(s):  
Jordan J. Lichty ◽  
Joshua L. Malecki ◽  
Heather D. Agnew ◽  
Daniel J. Michelson-Horowitz ◽  
Song Tan
Keyword(s):  
Protein Purification ◽  
Affinity Tags

WITHDRAWN: Reprint of: Comparison of affinity tags for protein purification

2011 ◽  
Author(s):  
Jordan J. Lichty ◽  
Joshua L. Malecki ◽  
Heather D. Agnew ◽  
Daniel J. Michelson-Horowitz ◽  
Song Tan
Keyword(s):  
Protein Purification ◽  
Affinity Tags

Recombinant Protein Purification Using Complementary Peptides as Affinity Tags

2010 ◽  
Vol 150 ◽  
pp. 352-352
Author(s):  
María C. Martínez Ceron ◽  
Alexandra M. Targovnik ◽  
Nicolás Urtasun ◽  
Osvaldo Cascone ◽  
María V. Miranda ◽  
...  
Keyword(s):  
Recombinant Protein ◽  
Protein Purification ◽  
Affinity Tags ◽  
Recombinant Protein Purification

Recombinant protein purification using complementary peptides as affinity tags

2012 ◽  
Vol 29 (2) ◽  
pp. 206-210 ◽  
Author(s):  
María C. Martínez-Ceron ◽  
Alexandra M. Targovnik ◽  
Nicolás Urtasun ◽  
Osvaldo Cascone ◽  
María V. Miranda ◽  
...  
Keyword(s):  
Recombinant Protein ◽  
Protein Purification ◽  
Affinity Tags ◽  
Recombinant Protein Purification
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