Maria L.L. Barreto do Nascimento
◽
Antonielly Campinho dos Reis
◽
José V.O. Santos
◽
Helber A. Negreiros
◽
Felipe C. Carneiro da Silva
◽
...
Background:
The search for novel metallic chemical compounds with toxicogenic effects have been of great
importance for more efficient cancer treatment.
Objective:
The study evaluated the cytotoxic, genotoxic and mutagenic activity of organoteluran RF07 in S-180 cell line.
Methods:
The bioassays used were cell viability with 3-(4,5-dimethyl-2-thiazole)-2,5-diphenyl-2-H-tetrazolium bromide
(MTT) test, evaluation of apoptosis and necrosis using fluorescence and flow cytometry, cytokinesis-block micronucleus
test and comet assay. The compound was tested at 1; 2.5 and 5 µM.
Results:
The results showed the cytotoxicity of RF07 at concentrations of 2.5, 5, 10 and 20 µM when compared to the
negative control. For genotoxicity tests, RF07 showed effects in all concentrations assessed by increased index and
frequencies of damage and mutagenic alterations. The compound was also cytotoxic due to the significant decrease in
nuclear division index, with significant values of apoptosis and necrosis. The results of fluorescence and flow cytometry
showed apoptosis as the main type of cell death caused by RF07 at 5 µM, which is thought to avoid an aggressive immune
response of the organism.
Conclusion:
In addition to cytotoxic and genotoxic effects, RF07 creates good perspectives for future antitumor
formulations.
