Amplified restrictionfragmentlengthpolymorphism technique (AFLP technique; amplified fragment length polymorphism technique)

2015 ◽  
pp. 1-1
Keyword(s):  
Length Polymorphism ◽  
Fragment Length ◽  
Fragment Length Polymorphism ◽  
Amplified Fragment Length Polymorphism ◽  
Aflp Technique

Amplified fragment length polymorphism (AFLP) versus randomly amplified polymorphic DNA (RAPD) as new tools for inter- and intra-species differentiation within Bordetella

2005 ◽  
Vol 54 (4) ◽  
pp. 333-346 ◽  
Author(s):  
Anna Gzyl ◽  
Ewa Augustynowicz ◽  
Ewa Mosiej ◽  
Monika Zawadka ◽  
Grzegorz Gniadek ◽  
...  
Keyword(s):  
Length Polymorphism ◽  
Fragment Length ◽  
Fragment Length Polymorphism ◽  
Amplified Fragment Length Polymorphism ◽  
Discriminatory Power ◽  
Species Discrimination ◽  
Species Differentiation ◽  
Randomly Amplified Polymorphic Dna ◽  
Precise Data ◽  
Aflp Technique

Automated amplified fragment length polymorphism (AFLP) and randomly amplified polymorphic DNA (RAPD) techniques with fluorescently labelled primers were used to track differences among isolates of the eight known species of the Bordetella genus. Eighty-one representative strains of these species from international and Polish bacterial collections were genotyped according to RAPD protocols using primer 1254 or 1247, and AFLP involving EcoRI/MseI or newly designed SpeI/ApaI restriction/ligation/amplification procedures. By comparing AFLP and RAPD data, it was concluded that the discriminatory power of AFLP is higher in comparison with RAPD for both intra- and inter-species differentiation of isolates of the Bordetella genus. The most precise level of inter-species discrimination and the highest level of intra-species discrimination of the Bordetella isolates of the eight species were observed in the AFLP EcoRI/MseI and SpeI/ApaI sets, respectively. Both techniques might provide alternative tools for the identification of Bordetella at the genomic species and strain levels, and thus may be valuable in human and veterinary diagnostics as well as in epidemiology. By applying the AFLP technique presented in this article, more precise data on the emergence of newly acquired and/or on expanded clones and transmission routes of isolates of the Bordetella genus in the human and animal environments might be obtained.

Evaluation of a modified single-enzyme amplified fragment length polymorphism (SE-AFLP) technique for subtyping Salmonella enterica serotype Enteritidis

2007 ◽  
Vol 158 (1) ◽  
pp. 10-17 ◽  
Author(s):  
Giovanni M. Giammanco ◽  
Caterina Mammina ◽  
Cristina Romani ◽  
Ida Luzzi ◽  
Anna Maria Dionisi ◽  
...  
Keyword(s):  
Length Polymorphism ◽  
Fragment Length ◽  
Salmonella Enterica ◽  
Fragment Length Polymorphism ◽  
Amplified Fragment Length Polymorphism ◽  
Single Enzyme ◽  
Aflp Technique ◽  
Salmonella Enterica Serotype Enteritidis

scholarly journals Association of nymphs and adults of Ephemeroptera (Insecta) using the amplified fragment length polymorphism (AFLP) technique

2011 ◽  
Vol 47 (2) ◽  
pp. 151-157 ◽  
Author(s):  
Lucimar G. Dias ◽  
Tito Bacca ◽  
Lucio Navarro ◽  
Flor E. Acevedo ◽  
Pablo M. Benavides ◽  
...  
Keyword(s):  
Length Polymorphism ◽  
Fragment Length ◽  
Fragment Length Polymorphism ◽  
Amplified Fragment Length Polymorphism ◽  
Aflp Technique

Genetic diversity of the endangered Chinese endemic herb Primulina tabacum (Gesneriaceae) revealed by amplified fragment length polymorphism (AFLP)

Genetica ◽  
2006 ◽  
Vol 127 (1-3) ◽  
pp. 177-183 ◽  
Author(s):  
Xiaowei Ni ◽  
Yelin Huang ◽  
Lin Wu ◽  
Renchao Zhou ◽  
Shulin Deng ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Length Polymorphism ◽  
Fragment Length ◽  
Fragment Length Polymorphism ◽  
Amplified Fragment Length Polymorphism ◽  
Primulina Tabacum

Amplified Fragment Length Polymorphism in a Selected Sample of Soft Red Winter Wheat

2001 ◽  
Vol 29 (3-4) ◽  
pp. 251-258 ◽  
Author(s):  
Anne M. Grünberg ◽  
José M. Costa ◽  
Robert J. Kratochvil
Keyword(s):  
Winter Wheat ◽  
Length Polymorphism ◽  
Fragment Length ◽  
Fragment Length Polymorphism ◽  
Amplified Fragment Length Polymorphism ◽  
Soft Red Winter Wheat ◽  
Red Winter Wheat
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