Spun-column procedure

Abstract A single-column procedure, using Type UR-30 resin to develop acidic, neutral, and basic amino acids normally found in protein or peptide hydrolyzates is reported for the study, in human serum, of amino acids associated with some aminoacidopathies. Using a sodium citrate, three-buffer, step-change system, pH 3.28 (0.20 N), pH 4.30 (0.20 N), and pH 6.71 (1.0 N), the acidic and neutral amino acids are chromato-graphed in 85 min. A complete analysis including the basic amino acids requires 135 min. This resin also will perform the function of earlier resins—ie, the separation of the acidic and neutral amino acids by the simple hydrolyzate procedures and by the more complex physiologic procedures.

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Increased creatine kinase isoenzyme MB values in patients without myocardial infarct.

Clinical Chemistry ◽

10.1093/clinchem/24.10.1818 ◽

1978 ◽

Vol 24 (10) ◽

pp. 1818-1821 ◽

Cited By ~ 3

Author(s):

L M Shaw ◽

D A Newman

Keyword(s):

Myocardial Infarction ◽

Intensive Care Unit ◽

Creatine Kinase ◽

Medical Intensive Care Unit ◽

Myocardial Infarct ◽

Creatine Kinase Isoenzyme ◽

Creatine Kinase Mb ◽

Medical Intensive Care ◽

Column Procedure ◽

Creatine Kinase Isoenzyme Mb

Abstract Six of 13 randomly selected patients in a medical intensive-care unit with above-normal creatine kinase MB activities had diagnoses other than myocardial infarction. These data, which indicate the need for further study, were obtained during evaluation of a commercially available column procedure (Biodynamics/bmc).

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Solid-phase extraction for the screening of acidic, neutral and basic drugs in plasma using a single-column procedure on Bond Elut Certify

Journal of Chromatography B Biomedical Sciences and Applications ◽

10.1016/s0378-4347(00)83817-7 ◽

1990 ◽

Vol 529 ◽

pp. 161-166 ◽

Cited By ~ 43

Author(s):

Xiaohua Chen ◽

Jaap Wijsbeek ◽

Jan Van Veen ◽

Jan Piet Franke ◽

Rokus A. De Zeeuw

Keyword(s):

Solid Phase Extraction ◽

Solid Phase ◽

Phase Extraction ◽

Basic Drugs ◽

Single Column ◽

Neutral And Basic Drugs ◽

Column Procedure

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Separation of Various Forms of Nitrogen in Fertilizers

Journal of AOAC INTERNATIONAL ◽

10.1093/jaoac/47.6.1054 ◽

1964 ◽

Vol 47 (6) ◽

pp. 1054-1056

Author(s):

J M O’Neal ◽

K G Clark

Keyword(s):

Anion Exchange ◽

Water Soluble ◽

Anion Exchange Column ◽

Exchange Column ◽

Phosphate Ion ◽

Anion Resin ◽

Precipitation Procedure ◽

Aoac Method ◽

Column Procedure

Abstract A method is proposed in which both a cation and an anion resin are used to separate the various forms of nitrogen in fertilizers. By this method troublesome phosphate ion is removed from the urea-containing solution, thereby making it possible to determine urea directly without the double-precipitation procedure required in AOAC method 2.055. The proposed cation-anion exchange column procedure makes it possible to determine the water-soluble amnionic, nitric, and amidic forms of nitrogen rapidly, accurately, and directly.

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