Three-in-One Chromatography-Free Purification, Tag Removal, and Site-Specific Modification of Recombinant Fusion Proteins Using Sortase A and Elastin-like Polypeptides

2013 ◽  
Vol 125 (13) ◽  
pp. 3791-3796 ◽  
Author(s):  
Joseph J. Bellucci ◽  
Miriam Amiram ◽  
Jayanta Bhattacharyya ◽  
Dewey McCafferty ◽  
Ashutosh Chilkoti
Keyword(s):  
Fusion Proteins ◽  
Sortase A ◽  
Site Specific ◽  
Specific Modification ◽  
Recombinant Fusion Proteins

scholarly journals Three-in-One Chromatography-Free Purification, Tag Removal, and Site-Specific Modification of Recombinant Fusion Proteins Using Sortase A and Elastin-like Polypeptides

2013 ◽  
Vol 52 (13) ◽  
pp. 3703-3708 ◽  
Author(s):  
Joseph J. Bellucci ◽  
Miriam Amiram ◽  
Jayanta Bhattacharyya ◽  
Dewey McCafferty ◽  
Ashutosh Chilkoti
Keyword(s):  
Fusion Proteins ◽  
Sortase A ◽  
Site Specific ◽  
Specific Modification ◽  
Recombinant Fusion Proteins

scholarly journals New Method for Site-Specific Modification of Liposomes with Proteins Using Sortase A-Mediated Transpeptidation

2012 ◽  
Vol 23 (3) ◽  
pp. 650-655 ◽  
Author(s):  
Xueqing Guo ◽  
Zhimeng Wu ◽  
Zhongwu Guo
Keyword(s):  
New Method ◽  
Sortase A ◽  
Site Specific ◽  
Specific Modification

scholarly journals Introduction of spacer peptides N-terminal to a cleavage recognition motif in recombinant fusion proteins can improve site-specific cleavage

1997 ◽  
Vol 10 (6) ◽  
pp. 615-619 ◽  
Author(s):  
S. W. Polyak ◽  
G. Forsberg ◽  
B. E. Forbes ◽  
K. A. McNeil ◽  
S. E. Aplin ◽  
...  
Keyword(s):  
Fusion Proteins ◽  
Site Specific ◽  
Recombinant Fusion Proteins ◽  
Recognition Motif

[19] Production of site-specific P450 antibodies using recombinant fusion proteins as antigens

1991 ◽  
pp. 193-201 ◽  
Author(s):  
Thomas Friedberg ◽  
Wolfgang Kissel ◽  
Michael Arand ◽  
Franz Oesch
Keyword(s):  
Fusion Proteins ◽  
Site Specific ◽  
Recombinant Fusion Proteins

Site-specific modification of E. coli DNA polymerase I large fragment with pyridoxal 5'-phosphate

Biochemistry ◽  
1984 ◽  
Vol 23 (9) ◽  
pp. 2073-2078 ◽  
Author(s):  
Anup K. Hazra ◽  
Sevilla Detera-Wadleigh ◽  
Samuel H. Wilson
Keyword(s):  
Dna Polymerase ◽  
Large Fragment ◽  
Dna Polymerase I ◽  
Site Specific ◽  
E Coli ◽  
Specific Modification ◽  
Polymerase I

scholarly journals Dual, Site-Specific Modification of Antibodies by Using Solid-Phase Immobilized Microbial Transglutaminase

ChemBioChem ◽  
2017 ◽  
Vol 18 (19) ◽  
pp. 1923-1927 ◽  
Author(s):  
Philipp R. Spycher ◽  
Christian A. Amann ◽  
Jöri E. Wehrmüller ◽  
David R. Hurwitz ◽  
Olivier Kreis ◽  
...  
Keyword(s):  
Solid Phase ◽  
Microbial Transglutaminase ◽  
Site Specific ◽  
Specific Modification ◽  
Dual Site

scholarly journals Expression of ΔF508 CFTR in normal mouse lung after site-specific modification of CFTR sequences by SFHR

Gene Therapy ◽  
2001 ◽  
Vol 8 (12) ◽  
pp. 961-965 ◽  
Author(s):  
KK Goncz ◽  
A Colosimo ◽  
B Dallapiccola ◽  
L Gagné ◽  
K Hong ◽  
...  
Keyword(s):  
Normal Mouse ◽  
Mouse Lung ◽  
Site Specific ◽  
Specific Modification ◽  
Δf508 Cftr

Use of recombinant fusion proteins for generation and rapid characterization of monoclonal antibodies

1992 ◽  
Vol 147 (1) ◽  
pp. 1-11 ◽  
Author(s):  
D. Wunderlich ◽  
A. Lee ◽  
R.P. Fracasso ◽  
D.V. Mierz ◽  
R.M. Bayney ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Fusion Proteins ◽  
Recombinant Fusion Proteins ◽  
Rapid Characterization
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