scholarly journals Spatiotemporally separated cardiac neural crest subpopulations that target the outflow tract septum and pharyngeal arch arteries

2003 ◽  
Vol 275A (1) ◽  
pp. 1009-1018 ◽  
Author(s):  
Marit J. Boot ◽  
Adriana C. Gittenberger-De Groot ◽  
Liesbeth Van Iperen ◽  
Beerend P. Hierck ◽  
Robert E. Poelmann
Keyword(s):  
Neural Crest ◽  
Outflow Tract ◽  
Pharyngeal Arch ◽  
Cardiac Neural Crest ◽  
Pharyngeal Arch Arteries

Fate of the mammalian cardiac neural crest

Development ◽  
2000 ◽  
Vol 127 (8) ◽  
pp. 1607-1616 ◽  
Author(s):  
X. Jiang ◽  
D.H. Rowitch ◽  
P. Soriano ◽  
A.P. McMahon ◽  
H.M. Sucov
Keyword(s):  
Neural Crest ◽  
Genetic System ◽  
Outflow Tract ◽  
Substantial Contribution ◽  
Pharyngeal Arch ◽  
Postnatal Life ◽  
Cardiac Neural Crest ◽  
Mammalian Embryos ◽  
Pharyngeal Arch Arteries

A subpopulation of neural crest termed the cardiac neural crest is required in avian embryos to initiate reorganization of the outflow tract of the developing cardiovascular system. In mammalian embryos, it has not been previously experimentally possible to study the long-term fate of this population, although there is strong inference that a similar population exists and is perturbed in a number of genetic and teratogenic contexts. We have employed a two-component genetic system based on Cre/lox recombination to label indelibly the entire mouse neural crest population at the time of its formation, and to detect it at any time thereafter. Labeled cells are detected throughout gestation and in postnatal stages in major tissues that are known or predicted to be derived from neural crest. Labeling is highly specific and highly efficient. In the region of the heart, neural-crest-derived cells surround the pharyngeal arch arteries from the time of their formation and undergo an altered distribution coincident with the reorganization of these vessels. Labeled cells populate the aorticopulmonary septum and conotruncal cushions prior to and during overt septation of the outflow tract, and surround the thymus and thyroid as these organs form. Neural-crest-derived mesenchymal cells are abundantly distributed in midgestation (E9.5-12.5), and adult derivatives of the third, fourth and sixth pharyngeal arch arteries retain a substantial contribution of labeled cells. However, the population of neural-crest-derived cells that infiltrates the conotruncus and which surrounds the noncardiac pharyngeal organs is either overgrown or selectively eliminated as development proceeds, resulting for these tissues in a modest to marginal contribution in late fetal and postnatal life.

scholarly journals Foxc2 is required for proper cardiac neural crest cell migration, outflow tract septation, and ventricle expansion

2018 ◽  
Vol 247 (12) ◽  
pp. 1286-1296 ◽  
Author(s):  
Kimberly E. Inman ◽  
Carlo Donato Caiaffa ◽  
Kristin R. Melton ◽  
Lisa L. Sandell ◽  
Annita Achilleos ◽  
...  
Keyword(s):  
Cell Migration ◽  
Neural Crest ◽  
Neural Crest Cell ◽  
Outflow Tract ◽  
Cardiac Neural Crest ◽  
Neural Crest Cell Migration ◽  
Crest Cell

Pax3 is required for cardiac neural crest migration in the mouse: evidence from the splotch (Sp2H) mutant

Development ◽  
1997 ◽  
Vol 124 (2) ◽  
pp. 505-514 ◽  
Author(s):  
S.J. Conway ◽  
D.J. Henderson ◽  
A.J. Copp
Keyword(s):  
Neural Crest ◽  
Neural Tube ◽  
Neural Crest Cells ◽  
Outflow Tract ◽  
Avian Embryo ◽  
Cardiac Neural Crest ◽  
Branchial Arches ◽  
Aortic Arches ◽  
Cardiac Outflow

Neural crest cells originating in the occipital region of the avian embryo are known to play a vital role in formation of the septum of the cardiac outflow tract and to contribute cells to the aortic arches, thymus, thyroid and parathyroids. This ‘cardiac’ neural crest sub-population is assumed to exist in mammals, but without direct evidence. In this paper we demonstrate, using RT-PCR and in situ hybridisation, that Pax3 expression can serve as a marker of cardiac neural crest cells in the mouse embryo. Cells of this lineage were traced from the occipital neural tube, via branchial arches 3, 4 and 6, into the aortic sac and aorto-pulmonary outflow tract. Confirmation that these Pax3-positive cells are indeed cardiac neural crest is provided by experiments in which hearts were deprived of a source of colonising neural crest, by organ culture in vitro, with consequent lack of up-regulation of Pax3. Occipital neural crest cell outgrowths in vitro were also shown to express Pax3. Mutation of Pax3, as occurs in the splotch (Sp2H) mouse, results in development of conotruncal heart defects including persistent truncus arteriosus. Homozygotes also exhibit defects of the aortic arches, thymus, thyroid and parathyroids. Pax3-positive neural crest cells were found to emigrate from the occipital neural tube of Sp2H/Sp2H embryos in a relatively normal fashion, but there was a marked deficiency or absence of neural crest cells traversing branchial arches 3, 4 and 6, and entering the cardiac outflow tract. This decreased expression of Pax3 in Sp2H/Sp2H embryos was not due to down-regulation of Pax3 in neural crest cells, as use of independent neural crest markers, Hoxa-3, CrabpI, Prx1, Prx2 and c-met also revealed a deficiency of migrating cardiac neural crest cells in homozygous embryos. This work demonstrates the essential role of the cardiac neural crest in formation of the heart and great vessels in the mouse and, furthermore, shows that Pax3 function is required for the cardiac neural crest to complete its migration to the developing heart.

scholarly journals Msx1 and Msx2 regulate survival of secondary heart field precursors and post-migratory proliferation of cardiac neural crest in the outflow tract

2007 ◽  
Vol 308 (2) ◽  
pp. 421-437 ◽  
Author(s):  
Yi-Hui Chen ◽  
Mamoru Ishii ◽  
Jingjing Sun ◽  
Henry M. Sucov ◽  
Robert E. Maxson
Keyword(s):  
Neural Crest ◽  
Outflow Tract ◽  
Cardiac Neural Crest ◽  
Heart Field ◽  
Secondary Heart Field

scholarly journals Prediction and characterisation of a highly conserved, remote and cAMP responsive enhancer that regulates Msx1 gene expression in cardiac neural crest and outflow tract

2008 ◽  
Vol 317 (2) ◽  
pp. 686-694 ◽  
Author(s):  
Kerry Ann Miller ◽  
Scott Davidson ◽  
Angela Liaros ◽  
John Barrow ◽  
Marissa Lear ◽  
...  
Keyword(s):  
Gene Expression ◽  
Neural Crest ◽  
Outflow Tract ◽  
Cardiac Neural Crest

scholarly journals Cardiac outflow tract septation failure in Pax3-deficient embryos is due to p53-dependent regulation of migrating cardiac neural crest

2008 ◽  
Vol 125 (9-10) ◽  
pp. 757-767 ◽  
Author(s):  
Sarah C. Morgan ◽  
Hyung-Yul Lee ◽  
Frédéric Relaix ◽  
Lisa L. Sandell ◽  
John M. Levorse ◽  
...  
Keyword(s):  
Neural Crest ◽  
Outflow Tract ◽  
Cardiac Neural Crest ◽  
Cardiac Outflow

scholarly journals Pax3 is essential for normal cardiac neural crest morphogenesis but is not required during migration nor outflow tract septation

2011 ◽  
Vol 356 (2) ◽  
pp. 308-322 ◽  
Author(s):  
Michael Olaopa ◽  
Hong-ming Zhou ◽  
Paige Snider ◽  
Jian Wang ◽  
Robert J. Schwartz ◽  
...  
Keyword(s):  
Neural Crest ◽  
Outflow Tract ◽  
Cardiac Neural Crest

scholarly journals Regulation of Sema3c and the Interaction between Cardiac Neural Crest and Second Heart Field during Outflow Tract Development

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Kazuki Kodo ◽  
Shinsuke Shibata ◽  
Sachiko Miyagawa-Tomita ◽  
Sang-Ging Ong ◽  
Hiroshi Takahashi ◽  
...  
Keyword(s):  
Neural Crest ◽  
Outflow Tract ◽  
Cardiac Neural Crest ◽  
Second Heart Field ◽  
Heart Field
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