scholarly journals The self-sufficient P450 RhF expressed in a whole cell system selectively catalyses the 5-hydroxylation of diclofenac

2017 ◽  
Vol 12 (3) ◽  
pp. 1600520 ◽  
Author(s):  
Jan M. Klenk ◽  
Bernd A. Nebel ◽  
Joanne L. Porter ◽  
Justyna K. Kulig ◽  
Shaneela A. Hussain ◽  
...  
Keyword(s):  
The Self ◽  
Author(s):  
Yeong-Hoon Han ◽  
Hyun Joong Kim ◽  
Tae-Rim Choi ◽  
Hun-Suk Song ◽  
Sun Mi Lee ◽  
...  

2020 ◽  
Vol 22 (13) ◽  
pp. 4128-4132 ◽  
Author(s):  
Stylianos Grigoriou ◽  
Pierre Kugler ◽  
Evelina Kulcinskaja ◽  
Frederik Walter ◽  
John King ◽  
...  

The development and application of a self-sufficient whole-cell system for transaminase biotransformations is described. The system relies on an engineered strain of Corynebacterium glutamicum that produces smart amine donors.


1992 ◽  
Vol 3 (12) ◽  
pp. 1543-1546 ◽  
Author(s):  
Mark A. Cohen ◽  
Julian S. Parratt ◽  
Nicholas J. Turner

2015 ◽  
Vol 51 (90) ◽  
pp. 16173-16175 ◽  
Author(s):  
Thomas H. Lonsdale ◽  
Lars Lauterbach ◽  
Sumire Honda Malca ◽  
Bettina M. Nestl ◽  
Bernhard Hauer ◽  
...  

A bacterial whole-cell system was designed for hydroxylation of n-octane to 1-octanol at the expense of molecular hydrogen and oxygen.


Authorea ◽  
2020 ◽  
Author(s):  
Yeong Hoon Han ◽  
Tae Rim Choi ◽  
Ye Lim Park ◽  
Jun Young Park ◽  
Hun suk Song ◽  
...  

2021 ◽  
Author(s):  
Yu-Chang Liu ◽  
Zhong-Liu Wu ◽  
Jan Deska

<p>Following a synthetic chemistry blueprint for the valorization of lignocellulosic platform chemicals, this study showcases a so far unprecedented approach to implement non-natural enzyme modules in vivo. For the design of a novel functional whole cell tool, two purely abiotic transformations were incorporated into a recombinant bacterial host that allows production of complex lactone building blocks. This whole cell system streamlines the synthetic cascade, eliminates isolation and purification steps, and provides a high degree stereoselectivity that has so far been elusive in the chemical methodology.</p>


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