H2-driven biotransformation of n-octane to 1-octanol by a recombinant Pseudomonas putida strain co-synthesizing an O2-tolerant hydrogenase and a P450 monooxygenase

ABSTRACT A new cytochrome P450 monooxygenase, FcpC, from Streptomyces virginiae IBL-14 has been identified. This enzyme is found to be responsible for the bioconversion of a pyrano-spiro steroid (diosgenone) to a rare nuatigenin-type spiro steroid (isonuatigenone), which is a novel C-25-hydroxylated diosgenone derivative. A whole-cell P450 system was developed for the production of isonuatigenone via the expression of the complete three-component electron transfer chain in an Escherichia coli strain.

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Development and evaluation of a whole-cell system for the directed evolution of human cytochromes P450 in recombinant fission yeast Schizosaccharomyces pombe

Journal of Molecular Catalysis B Enzymatic ◽

10.1016/j.molcatb.2013.08.011 ◽

2014 ◽

Vol 103 ◽

pp. 67-71

Author(s):

Tarek Hakki ◽

Rita Bernhardt

Keyword(s):

Schizosaccharomyces Pombe ◽

Fission Yeast ◽

Directed Evolution ◽

Cytochromes P450 ◽

Cell System ◽

Whole Cell

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The self-sufficient P450 RhF expressed in a whole cell system selectively catalyses the 5-hydroxylation of diclofenac

Biotechnology Journal ◽

10.1002/biot.201600520 ◽

2017 ◽

Vol 12 (3) ◽

pp. 1600520 ◽

Cited By ~ 16

Author(s):

Jan M. Klenk ◽

Bernd A. Nebel ◽

Joanne L. Porter ◽

Justyna K. Kulig ◽

Shaneela A. Hussain ◽

...

Keyword(s):

Cell System ◽

The Self ◽

Whole Cell

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Enhanced production of cadaverine by the addition of hexadecyltrimethylammonium bromide to whole cell system with regeneration of pyridoxal-5′-phosphate and ATP

Enzyme and Microbial Technology ◽

10.1016/j.enzmictec.2019.04.010 ◽

2019 ◽

Vol 127 ◽

pp. 58-64 ◽

Cited By ~ 11

Author(s):

Yu-Mi Moon ◽

Soo Yeon Yang ◽

Tae Rim Choi ◽

Hye-Rim Jung ◽

Hun-Suk Song ◽

...

Keyword(s):

Cell System ◽

Hexadecyltrimethylammonium Bromide ◽

Whole Cell ◽

Enhanced Production

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Characterization of wastewater toxicity by means of a whole-cell bacterial biosensor, using Pseudomonas putida, in conjunction with chemical analysis

Fresenius Journal of Analytical Chemistry ◽

10.1007/s002160100925 ◽

2001 ◽

Vol 371 (4) ◽

pp. 467-473 ◽

Cited By ~ 18

Author(s):

Marinella Farré ◽

Damià Barceló

Keyword(s):

Chemical Analysis ◽

Pseudomonas Putida ◽

Bacterial Biosensor ◽

Whole Cell

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Vaccination efficiency of surface antigens and killed whole cell of Pseudomonas putida in large yellow croaker (Pseudosciaena crocea)

Fish & Shellfish Immunology ◽

10.1016/j.fsi.2013.04.030 ◽

2013 ◽

Vol 35 (2) ◽

pp. 375-381 ◽

Cited By ~ 6

Author(s):

Zhijuan Mao ◽

Jian Ye ◽

Meifang Li ◽

Huiying Xu ◽

Jigang Chen

Keyword(s):

Pseudomonas Putida ◽

Surface Antigens ◽

Large Yellow Croaker ◽

Pseudosciaena Crocea ◽

Whole Cell

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Development of a Corynebacterium glutamicum bio-factory for self-sufficient transaminase reactions

Green Chemistry ◽

10.1039/d0gc01432j ◽

2020 ◽

Vol 22 (13) ◽

pp. 4128-4132 ◽

Cited By ~ 2

Author(s):

Stylianos Grigoriou ◽

Pierre Kugler ◽

Evelina Kulcinskaja ◽

Frederik Walter ◽

John King ◽

...

Keyword(s):

Corynebacterium Glutamicum ◽

Cell System ◽

Whole Cell ◽

Engineered Strain

The development and application of a self-sufficient whole-cell system for transaminase biotransformations is described. The system relies on an engineered strain of Corynebacterium glutamicum that produces smart amine donors.

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Enantioselective hydrolysis of nitriles and amides using an immobilised whole cell system

Tetrahedron Asymmetry ◽

10.1016/s0957-4166(00)86056-5 ◽

1992 ◽

Vol 3 (12) ◽

pp. 1543-1546 ◽

Cited By ~ 31

Author(s):

Mark A. Cohen ◽

Julian S. Parratt ◽

Nicholas J. Turner

Keyword(s):

Cell System ◽

Enantioselective Hydrolysis ◽

Whole Cell ◽

Hydrolysis Of

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Biosynthesis of Nylon 12 Monomer, ω-Aminododecanoic Acid Using Artificial Self-Sufficient P450, AlkJ and ω-TA

Catalysts ◽

10.3390/catal8090400 ◽

2018 ◽

Vol 8 (9) ◽

pp. 400 ◽

Cited By ~ 9

Author(s):

Md Ahsan ◽

Mahesh Patil ◽

Hyunwoo Jeon ◽

Sihyong Sung ◽

Taeowan Chung ◽

...

Keyword(s):

Cytochrome P450 ◽

Pseudomonas Putida ◽

Single Cell ◽

High Performance ◽

Cell System ◽

Dodecanoic Acid ◽

Cytochrome P450 Reductase ◽

One Pot ◽

Renewable Sources ◽

Nylon 12

ω-Aminododecanoic acid is considered as one of the potential monomers of Nylon 12, a high-performance member of the bioplastic family. The biosynthesis of ω-aminododecanoic acid from renewable sources is an attractive process in the polymer industry. Here, we constructed three artificial self-sufficient P450s (ArtssP450s) using CYP153A13 from Alcanivorax borkumensis and cytochrome P450 reductase (CPR) domains of natural self-sufficient P450s (CYP102A1, CYP102A5, and 102D1). Among them, artificial self-sufficient P450 (CYP153A13BM3CPR) with CYP102A1 CPR showed the highest catalytically activity for dodecanoic acid (DDA) substrate. This form of ArtssP450 was further co-expressed with ω-TA from Silicobacter pomeroyi and AlkJ from Pseudomonas putida GPo1. This single-cell system was used for the biotransformation of dodecanoic acid (DDA) to ω-aminododecanoic acid (ω-AmDDA), wherein we could successfully biosynthesize 1.48 mM ω-AmDDA from 10 mM DDA substrate in a one-pot reaction. The productivity achieved in the present study was five times higher than that achieved in our previously reported multistep biosynthesis method (0.3 mM).

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