Recent advances in engineering of microbial cell factories for intelligent pH regulation and tolerance

2021 ◽  
pp. 2100151
Author(s):  
Xiaopeng Gao ◽  
Ke Xu ◽  
Nadeem Ahmad ◽  
Lei Qin ◽  
Chun Li
Keyword(s):  
Ph Regulation ◽  
Microbial Cell ◽  
Microbial Cell Factories ◽  
Cell Factories ◽  
Recent Advances

scholarly journals Recent advances in improving metabolic robustness of microbial cell factories

2020 ◽  
Vol 66 ◽  
pp. 69-77 ◽  
Author(s):  
Tian Jiang ◽  
Chenyi Li ◽  
Yuxi Teng ◽  
Ruihua Zhang ◽  
Yajun Yan
Keyword(s):  
Microbial Cell ◽  
Microbial Cell Factories ◽  
Cell Factories ◽  
Recent Advances ◽  
Metabolic Robustness

scholarly journals Engineering of Microbial Cell Factories for the Production of Plant Polyphenols with Health-Beneficial Properties

2018 ◽  
Vol 24 (19) ◽  
pp. 2208-2225 ◽  
Author(s):  
Alexey Dudnik ◽  
Paula Gaspar ◽  
Ana Rute Neves ◽  
Jochen Forster
Keyword(s):  
Microbial Cell ◽  
Plant Polyphenols ◽  
Potential Health ◽  
Production Methods ◽  
Success Stories ◽  
Microbial Cell Factories ◽  
Cell Factories ◽  
Recent Advances ◽  
Current Production ◽  
The Common

Polyphenols form a group of important natural bioactive compounds with numerous ascribed healthbeneficial attributes (e.g. antioxidant, anti-inflammatory, anti-microbial and tumor-suppressing properties). Some polyphenols can also be used as natural dyes or plastic precursors. Notwithstanding their relevance, production of most of these compounds still relies on extraction from plant material, which for most of it is a costly and an inefficient procedure. The use of microbial cell factories for this purpose is an emerging alternative that could allow a more efficient and sustainable production. The most recent advances in molecular biology and genetic engineering, combined with the ever-growing understanding of microbial physiology have led to multiple success stories. Production of multiple polyphenolic compounds or their direct precursors has been achieved not only in the common production hosts, such as Escherichia coli and Saccharomyces cerevisiae, but also in Corynebacterium glutamicum and Lactococcus lactis. However, boosting production of native compounds or introduction of heterologous biosynthetic pathways also brings certain challenges, such as the need to express, balance and maintain efficient precursor supply. This review will discuss the most recent advances in the field of metabolic engineering of microorganisms for polyphenol biosynthesis and its future perspectives, as well as outlines their potential health benefits and current production methods.

Recent advances in tuning the expression and regulation of genes for constructing microbial cell factories

2021 ◽  
pp. 107767
Author(s):  
Seung-Woon Jung ◽  
Jinho Yeom ◽  
Jong Seong Park ◽  
Seung Min Yoo
Keyword(s):  
Microbial Cell ◽  
Microbial Cell Factories ◽  
Cell Factories ◽  
Recent Advances

Developing fourth-generation biofuels secreting microbial cell factories for enhanced productivity and efficient product recovery; a review

Fuel ◽  
2021 ◽  
Vol 298 ◽  
pp. 120858
Author(s):  
Sana Malik ◽  
Ayesha Shahid ◽  
Chen-Guang Liu ◽  
Aqib Zafar Khan ◽  
Muhammad Zohaib Nawaz ◽  
...  
Keyword(s):  
Product Recovery ◽  
Microbial Cell ◽  
Fourth Generation ◽  
Microbial Cell Factories ◽  
Cell Factories

scholarly journals Flux balance analysis indicates that methane is the lowest cost feedstock for microbial cell factories

2017 ◽  
Vol 5 ◽  
pp. 26-33 ◽  
Author(s):  
Austin D. Comer ◽  
Matthew R. Long ◽  
Jennifer L. Reed ◽  
Brian F. Pfleger
Keyword(s):  
Flux Balance Analysis ◽  
Microbial Cell ◽  
Flux Balance ◽  
Microbial Cell Factories ◽  
Cell Factories ◽  
Balance Analysis

scholarly journals Establishing new microbial cell factories for sustainable bioprocesses

2012 ◽  
Vol 29 ◽  
pp. S75-S76
Author(s):  
Mhairi Workman ◽  
Philippe Holt ◽  
Xiaoying Liu
Keyword(s):  
Microbial Cell ◽  
Microbial Cell Factories ◽  
Cell Factories

scholarly journals Recombinant production of eukaryotic cytochrome P450s in microbial cell factories

2018 ◽  
Vol 38 (2) ◽  
Author(s):  
Johanna Hausjell ◽  
Heidi Halbwirth ◽  
Oliver Spadiut
Keyword(s):  
Cytochrome P450s ◽  
Microbial Cell ◽  
Cytochrome P450 Reductase ◽  
Redox Partner ◽  
Microbial Cell Factories ◽  
Cell Factories ◽  
Recombinant Production ◽  
Membrane Bound ◽  
Essential Reactions ◽  
Synthesis Of Hormones

Cytochrome P450s (P450s) comprise one of the largest known protein families. They occur in every kingdom of life and catalyze essential reactions, such as carbon source assimilation, synthesis of hormones and secondary metabolites, or degradation of xenobiotics. Due to their outstanding ability of specifically hydroxylating complex hydrocarbons, there is a great demand to use these enzymes for biocatalysis, including applications at an industrial scale. Thus, the recombinant production of these enzymes is intensively investigated. However, especially eukaryotic P450s are difficult to produce. Challenges are faced due to complex cofactor requirements and the availability of a redox-partner (cytochrome P450 reductase, CPR) can be a key element to get active P450s. Additionally, most eukaryotic P450s are membrane bound which complicates the recombinant production. This review describes current strategies for expression of P450s in the microbial cell factories Escherichia coli, Saccharomyces cerevisiae, and Pichia pastoris.

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