Hydrogen-deuterium exchange in membrane proteins monitored by IR spectroscopy: A new tool to resolve protein structure and dynamics

Biopolymers ◽  
2004 ◽  
Vol 74 (1-2) ◽  
pp. 19-26 ◽  
Author(s):  
C. Vigano ◽  
M. Smeyers ◽  
V. Raussens ◽  
F. Scheirlinckx ◽  
J.M. Ruysschaert ◽  
...  
Keyword(s):  
Protein Structure ◽  
Membrane Proteins ◽  
Ir Spectroscopy ◽  
Deuterium Exchange ◽  
Hydrogen Deuterium Exchange ◽  
Structure And Dynamics ◽  
Hydrogen Deuterium ◽  
Protein Structure And Dynamics

New developments in protein structure-function analysis by MS and use of hydrogen-deuterium exchange microfluidics

FEBS Journal ◽  
2011 ◽  
Vol 278 (20) ◽  
pp. 3815-3821 ◽  
Author(s):  
Michael Landreh ◽  
Juan Astorga-Wells ◽  
Jan Johansson ◽  
Tomas Bergman ◽  
Hans Jörnvall
Keyword(s):  
Protein Structure ◽  
Structure Function ◽  
Function Analysis ◽  
Deuterium Exchange ◽  
Hydrogen Deuterium Exchange ◽  
New Developments ◽  
Hydrogen Deuterium

Dual-spray hydrogen/deuterium exchange (HDX) reactions: A new method of probing protein structure

2016 ◽  
Vol 30 (13) ◽  
pp. 1505-1512 ◽  
Author(s):  
Shaan Rashid ◽  
Sean Overton ◽  
Bihac Mazigh ◽  
Paul M. Mayer
Keyword(s):  
Protein Structure ◽  
Deuterium Exchange ◽  
New Method ◽  
Hydrogen Deuterium Exchange ◽  
Hydrogen Deuterium

scholarly journals Hydrogen-Deuterium Exchange Mass Spectrometry: A Novel Structural Biology Approach to Structure, Dynamics and Interactions of Proteins and Their Complexes

Life ◽  
2020 ◽  
Vol 10 (11) ◽  
pp. 286
Author(s):  
Oliver Ozohanics ◽  
Attila Ambrus
Keyword(s):  
Mass Spectrometry ◽  
Membrane Proteins ◽  
Structural Biology ◽  
Deuterium Exchange ◽  
Hydrogen Deuterium Exchange ◽  
Ligand Interaction ◽  
Exchange Mass Spectrometry ◽  
Hydrogen Deuterium ◽  
Deuterium Exchange Mass Spectrometry ◽  
Hdx Ms

Hydrogen/Deuterium eXchange Mass Spectrometry (HDX-MS) is a rapidly evolving technique for analyzing structural features and dynamic properties of proteins. It may stand alone or serve as a complementary method to cryo-electron-microscopy (EM) or other structural biology approaches. HDX-MS is capable of providing information on individual proteins as well as large protein complexes. Owing to recent methodological advancements and improving availability of instrumentation, HDX-MS is becoming a routine technique for some applications. When dealing with samples of low to medium complexity and sizes of less than 150 kDa, conformation and ligand interaction analyses by HDX-MS are already almost routine applications. This is also well supported by the rapid evolution of the computational (software) background that facilitates the analysis of the obtained experimental data. HDX-MS can cope at times with analytes that are difficult to tackle by any other approach. Large complexes like viral capsids as well as disordered proteins can also be analyzed by this method. HDX-MS has recently become an established tool in the drug discovery process and biopharmaceutical development, as it is now also capable of dissecting post-translational modifications and membrane proteins. This mini review provides the reader with an introduction to the technique and a brief overview of the most common applications. Furthermore, the most challenging likely applications, the analyses of glycosylated and membrane proteins, are also highlighted.

Improving the Sequence Coverage of Integral Membrane Proteins during Hydrogen/Deuterium Exchange Mass Spectrometry Experiments

2019 ◽  
Vol 91 (17) ◽  
pp. 10970-10978 ◽  
Author(s):  
Ingvar R. Möller ◽  
Marika Slivacka ◽  
Jiří Hausner ◽  
Anne Kathrine Nielsen ◽  
Eliška Pospíšilová ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Membrane Proteins ◽  
Deuterium Exchange ◽  
Integral Membrane Proteins ◽  
Hydrogen Deuterium Exchange ◽  
Sequence Coverage ◽  
Exchange Mass Spectrometry ◽  
Hydrogen Deuterium ◽  
Deuterium Exchange Mass Spectrometry
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