scholarly journals Electrostatic interaction between RNA and protein capsid in cowpea chlorotic mottle virus simulated by a coarse-grain RNA model and a Monte Carlo approach

Biopolymers ◽  
2004 ◽  
Vol 75 (4) ◽  
pp. 325-337 ◽  
Author(s):  
Deqiang Zhang ◽  
Robert Konecny ◽  
Nathan A. Baker ◽  
J. Andrew McCammon
Keyword(s):  
Monte Carlo ◽  
Electrostatic Interaction ◽  
Mottle Virus ◽  
Coarse Grain ◽  
Cowpea Chlorotic Mottle Virus ◽  
Monte Carlo Approach ◽  
Chlorotic Mottle Virus ◽  
Chlorotic Mottle

scholarly journals The Behaviour of Cowpea Chlorotic Mottle Virus in CsCl

1972 ◽  
Vol 15 (3) ◽  
pp. 247-251 ◽  
Author(s):  
J. B. Bancroft ◽  
I. H. Flack
Keyword(s):  
Mottle Virus ◽  
Cowpea Chlorotic Mottle Virus ◽  
Chlorotic Mottle Virus ◽  
Chlorotic Mottle

scholarly journals In Vitro Synthesis of Cowpea Chlorotic Mottle Virus Polypeptides

1979 ◽  
Vol 44 (2) ◽  
pp. 545-549 ◽  
Author(s):  
J. W. Davies ◽  
B. J. M. Verduin
Keyword(s):  
Mottle Virus ◽  
Cowpea Chlorotic Mottle Virus ◽  
In Vitro Synthesis ◽  
Chlorotic Mottle Virus ◽  
Chlorotic Mottle

Expression and Self Assembly of Cowpea Chlorotic Mottle Virus Capsid Proteins inPichia pastorisand Encapsulation of Fluorescent Myoglobin

2011 ◽  
Vol 1317 ◽  
Author(s):  
Yuanzheng Wu ◽  
Hetong Yang ◽  
Hyun-Jae Shin
Keyword(s):  
Coat Protein ◽  
Large Scale ◽  
Materials Science ◽  
Cesium Chloride ◽  
Size Exclusion ◽  
Mottle Virus ◽  
Cowpea Chlorotic Mottle Virus ◽  
Chlorotic Mottle Virus ◽  
Chlorotic Mottle

Abstract:Cowpea chlorotic mottle virus (CCMV) has been a model system for virus studies for over 40 years and now is considered to be a perfect candidate as nanoplatform for applications in materials science and medicine. The ability of CCMV to self assemblein vitrointo virus-like particles (VLPs) or capsids makes an ideal reaction vessel for nanomaterial synthesis and entrapment. Here we report expression of codon optimized CCMV coat protein inPichia pastorisand production of self assembled CCMV VLPs by large-scale fermentation. CCMV coat protein gene (573 bp) was synthesized according to codon preference ofP. pastorisand cloned into pPICZA vector. The recombinant plasmid pPICZA-CP was transformed intoP. pastorisGS115 by electroporation. The resulting yeast colonies were screened by PCR and analyzed for protein expression by SDS-PAGE. After large-scale fermentation CCMV coat protein yields reached 4.8 g L−1. The CCMV VLPs were purified by modified PEG precipitation followed by cesium chloride density gradient ultracentrifugation, and then analyzed by size exclusion fast performance liquid chromatography (FPLC), UV spectrometry and transmission electron microscopy. Myoglobin was used as a model protein to be encapsulated in CCMV VLPs. The fluorescence spectroscopy showed that inclusion of myoglobin had occurred. The results indicated the production of CCMV capsids byP. pastorisfermentation now available for utilization in pharmacology or nanotechnology fields.

Interaction with Capsid Protein Alters RNA Structure and the Pathway for In Vitro Assembly of Cowpea Chlorotic Mottle Virus

2004 ◽  
Vol 335 (2) ◽  
pp. 455-464 ◽  
Author(s):  
Jennifer M. Johnson ◽  
Deborah A. Willits ◽  
Mark J. Young ◽  
Adam Zlotnick
Keyword(s):  
Capsid Protein ◽  
Rna Structure ◽  
Mottle Virus ◽  
Cowpea Chlorotic Mottle Virus ◽  
Chlorotic Mottle Virus ◽  
Chlorotic Mottle ◽  
In Vitro Assembly

scholarly journals A Titrimetric and Electrophoretic Study of Cowpea Chlorotic Mottle Virus and its Protein

1973 ◽  
Vol 19 (2) ◽  
pp. 263-273 ◽  
Author(s):  
M. W. Johnson ◽  
G. W. Wagner ◽  
J. B. Bancroft
Keyword(s):  
Mottle Virus ◽  
Electrophoretic Study ◽  
Cowpea Chlorotic Mottle Virus ◽  
Chlorotic Mottle Virus ◽  
Chlorotic Mottle

Synchronization of Cowpea Chlorotic Mottle Virus Replication in Cowpea Leaves

Intervirology ◽  
1976 ◽  
Vol 7 (4-5) ◽  
pp. 284-291 ◽  
Author(s):  
William O. Dawson ◽  
David E. Schlegel
Keyword(s):  
Virus Replication ◽  
Mottle Virus ◽  
Cowpea Chlorotic Mottle Virus ◽  
Chlorotic Mottle Virus ◽  
Chlorotic Mottle

Photoresponsive, reversible immobilization of virus particles on supramolecular platforms

2017 ◽  
Vol 53 (11) ◽  
pp. 1896-1899 ◽  
Author(s):  
N. L. Weineisen ◽  
C. A. Hommersom ◽  
J. Voskuhl ◽  
S. Sankaran ◽  
A. M. A. Depauw ◽  
...  
Keyword(s):  
Mottle Virus ◽  
Cowpea Chlorotic Mottle Virus ◽  
Virus Particles ◽  
Chlorotic Mottle Virus ◽  
Chlorotic Mottle ◽  
Reversible Immobilization

Covalently attached azobenzene moieties to cowpea chlorotic mottle virus (CCMV) allow for photoresponsive immobilization on cucurbit[8]uril bearing surfaces.

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