Nature of foulants and fouling mechanism in the Protein A MabSelect resin cycled in a monoclonal antibody purification process

2015 ◽  
Vol 113 (1) ◽  
pp. 141-149 ◽  
Author(s):  
Shaojie Zhang ◽  
William Daniels ◽  
Jeffrey Salm ◽  
Judy Glynn ◽  
Joseph Martin ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Protein A ◽  
Purification Process ◽  
Antibody Purification ◽  
Fouling Mechanism

The dynamics of the CHO host cell protein profile during clarification and protein A capture in a platform antibody purification process

2012 ◽  
Vol 110 (1) ◽  
pp. 240-251 ◽  
Author(s):  
Catherine E.M. Hogwood ◽  
Andrew S. Tait ◽  
Nadejda Koloteva-Levine ◽  
Daniel G. Bracewell ◽  
C. Mark Smales
Keyword(s):  
Host Cell ◽  
Protein Profile ◽  
Protein A ◽  
Cell Protein ◽  
Purification Process ◽  
Host Cell Protein ◽  
Antibody Purification

Rapid Purification of Anti-SARS-CoV Protein N Monoclonal Antibody by Using Magnetic Beads

2011 ◽  
Vol 236-238 ◽  
pp. 2146-2149
Author(s):  
Yu Xi Yu ◽  
Xian Qiao Liu
Keyword(s):  
Monoclonal Antibody ◽  
Magnetic Beads ◽  
Protein A ◽  
Rabbit Serum ◽  
Purification Method ◽  
N Protein ◽  
Antibody Purification ◽  
Rapid Purification ◽  
Surface Modified ◽  
End Group

A rapid and simple antibody purification method is reported for the isolation of monoclonal antibody IgG1 from SARS-CoV N protein rabbit serum by using surface-modified magnetic beads. First magnetic poly (methacrylate-divinylbenzene) (PMA-DVB) beads were prepared and modified with aldehyde end group through surface chemical reactions. Then protein A were covalently bound to aldehyde-activated magnetic beads and further used to purify monoclonal antibody IgG1 from rabbit serum directly. This rapid magnetic carrier technology proves to be efficient and rapid for antibody purification and has potential to massive production of monoclonal antibody.

scholarly journals Membrane Adsorber for the Fast Purification of a Monoclonal Antibody Using Protein A Chromatography

Membranes ◽  
2019 ◽  
Vol 9 (12) ◽  
pp. 159 ◽  
Author(s):  
Chantal Brämer ◽  
Lisa Tünnermann ◽  
Alina Gonzalez Salcedo ◽  
Oscar-Werner Reif ◽  
Dörte Solle ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Continuous Process ◽  
Protein A ◽  
Capacity Utilization ◽  
Citrate Buffer ◽  
Cycle Times ◽  
Antibody Purification ◽  
Membrane Adsorber ◽  
Counter Current ◽  
Elution Step

Monoclonal antibodies are conquering the biopharmaceutical market because they can be used to treat a variety of diseases. Therefore, it is very important to establish robust and optimized processes for their production. In this article, the first step of chromatography (Protein A chromatography) in monoclonal antibody purification was optimized with a focus on the critical elution step. Therefore, different buffers (citrate, glycine, acetate) were tested for chromatographic performance and product quality. Membrane chromatography was evaluated because it promises high throughputs and short cycle times. The membrane adsorber Sartobind® Protein A 2 mL was used to accelerate the purification procedure and was further used to perform a continuous chromatographic run with a four-membrane adsorber-periodic counter-current chromatography (4MA-PCCC) system. It was found that citrate buffer at pH 3.5 and 0.15 M NaCl enabled the highest recovery of >95% and lowest total aggregate content of 0.26%. In the continuous process, the capacity utilization of the membrane adsorber was increased by 20%.

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