Purification Process
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2022 ◽  
Chi Zhang ◽  
Ming-Wen Hu ◽  
Shaoqiu He ◽  
Xuewei Wang ◽  
Xu Cao ◽  

Functionally distinct subtypes/clusters of dorsal root ganglion (DRG) neurons, which differ in soma size and neurochemical properties, may play different roles in nerve regeneration and pain. However, details about transcriptomic changes in different neuronal subtypes under maladaptive neuropathic pain conditions remain unclear. Chronic constriction injury (CCI) of the sciatic nerve represents a well-established model of neuropathic pain that mimics the etiology of clinical conditions. Therefore, we conducted single-cell RNA-sequencing (scRNA-seq) to characterize subtype-specific perturbations of transcriptomes in lumbar DRG neurons 7 days after sciatic CCI. By using Pirt-EGFPf mice that selectively express enhanced green fluorescent protein in DRG neurons, we established a highly efficient purification process to enrich neurons for scRNA-seq. We observed a loss of marker genes in injured neurons of 12 standard neuronal clusters, and the emergence of four prominent CCI-induced clusters at this peak-maintenance phase of neuropathic pain. Importantly, a portion of injured neurons from a subset of the 12 standard clusters (NP1, PEP5, NF1, and NF2) were spared from injury-induced identity loss, suggesting subtype-specific transcriptomic changes in injured neurons. Moreover, uninjured neurons, which are necessary for mediating the evoked pain, also demonstrated subtype-specific transcriptomic perturbations in these clusters, but not others. Notably, male and female mice showed differential transcriptomic changes in multiple neuronal clusters after CCI, suggesting transcriptomic sexual dimorphism in primary sensory neurons after nerve injury. Collectively, these findings may contribute to the identification of new target genes and development of DRG neuron subtype-specific therapies for optimizing neuropathic pain treatment and nerve regeneration.

2022 ◽  
pp. 2103495
Kwang Hee Kim ◽  
Chang‐Won Choi ◽  
Seokhyun Choung ◽  
Yoonjun Cho ◽  
Sungsoon Kim ◽  

Water ◽  
2022 ◽  
Vol 14 (1) ◽  
pp. 111
Ariadi Hazmi ◽  
Maulana Yusup Rosadi ◽  
Reni Desmiarti ◽  
Fusheng Li

Rainwater is a potential source of drinking water, but has various components of dissolved organic matter (DOM). DOM is a reservoir of potential hazards in drinking water. Therefore, a new method is required to purify rainwater as a drinking water source in terms of DOM aspects. A radio-frequency (RF) treatment system is introduced here to purify source water with a small possibility of contamination. RF is generated by applying a frequency of 1.5 MHz through a glass reactor with a diameter of 2 mm which is wrapped by a 2 mm copper wire. The results demonstrate that UV260 value and dissolved organic carbon (DOC) are reduced during RF treatment. DOC was reduced by a lower amount compared to UV260, suggesting the partial transformation of bio-refractory DOM. A fluorescence excitation-emission matrix showed that humic-like substances in rainwater were reduced faster than protein-like ones, indicating that humic-like substances are susceptible to reduction by RF treatment. The results offer information on the use of RF treatment in a rainwater purification process for the production of drinking water.

M. D. Ibragimova ◽  
H. J. Huseynov ◽  
R. Z. Hasanova

This paper presents the results of selective purification of oil distillates with a viscosity of 7.5 mm2/s and 18 mm2/s at [Formula: see text]C, as well as a mixture of these oil distillates obtained by compounding at various ratios using an ionic liquid based on diethylamine and formic acid as an extractant acid. The conditions for the ionic liquid dearomatization of the indicated oil distillates were determined by studying the influence of various factors on the selective purification process. Many parameters are set for the objects of research. The values of physicochemical parameters determined under various conditions are presented in the tables.

2022 ◽  
Vol 2160 (1) ◽  
pp. 012021
Jing Liu ◽  
Xiaobin Han ◽  
Yulong Peng ◽  
Xiaoyan Wang ◽  
Runmeng Qiao ◽  

Abstract Diatomite is a kind of natural material with adsorption capacity and it’s widely used in industry. The main purpose of this paper is to study the influence of hydrochloric acid concentration and modification time on the modification effect of diatomite. This article describes a modified method of diatomite, which can not only reduce the purification cost, but also improve the separation efficiency of solanesol. The results show that when the concentration of hydrochloric acid is 3mol/L and the treatment time is 50 minutes, the modification effect of diatomite is the best. The determination of the optimum technological conditions is conducive to the separation and purification of Solanesol by diatomite, and lays a foundation for the further development and utilization of diatomite.

2022 ◽  
Vol 334 ◽  
pp. 08010
Andrea Franzetti ◽  
Anna Espinoza ◽  
Enrico Barontini ◽  
Matteo Tucci ◽  
Paolo Bonelli ◽  

Planar MFC prototypes were constructed and experimented to operate as sensors of the anoxic condition in a denitrification tank of a wastewater treatment plant in Italy, during different times in 2018 – 2019. Electrodes were differently enriched with carbon paint containing nanotubes and CeO2 nanoparticles. Performances of different electrodes were compared. Results underline critical anoxic conditions in the tank, that caused a very low signal and phenomena of signal reversion during some period of the year. the activity of aerobic microorganisms and protozoa growing and grazing the bacteria on the electrodes strongly influenced the signal of the MFCs. The presence of nanoceria enhanced, for some extent, the MFC signal, both in presence of reversing trends and in absence of these phenomena. In absence of reversing trends, nanoceria enhanced the MFC voltage. Such signal trends from MFCs can give, in real-time, useful information to optimize the purification process without the necessity of frequent biological and chemical analyses.

Pharmaceutics ◽  
2022 ◽  
Vol 14 (1) ◽  
pp. 96
Maria Sinegubova ◽  
Ivan Vorobiev ◽  
Anatoly Klishin ◽  
Dmitry Eremin ◽  
Nadezhda Orlova ◽  

Recombinant human follicle stimulating hormone (r-hFSH) is widely used for infertility treatment and is subject to the development of biosimilars. There are different purification strategies that can yield r-hFSH of pharmaceutical quality from Chinese hamster ovary cell culture broth. We developed a purification process for r-hFSH centered on immunoaffinity chromatography with single-domain recombinant camelid antibodies. The resulting downstream process is simple and devoid of ultrafiltration operations. Studies on chromatography resin resource and ligand leakage showed that the immunoaffinity matrix employed was suitable for industrial use and stable for at least 40 full chromatography cycles, and the leaked single-domain antibody ligand was completely removed by subsequent purification steps. All chromatography resins employed withstood the same 40 cycles of use without significant changes in separation efficiency and product binding capacity. The resulting industrial purification process yielded batches of r-hFSH with consistent levels of purity and bioactivity.

Minerals ◽  
2021 ◽  
Vol 12 (1) ◽  
pp. 36
Patrycja Kowalik ◽  
Dorota Kopyto ◽  
Mateusz Ciszewski ◽  
Michał Drzazga ◽  
Katarzyna Leszczyńska-Sejda

This work focused on purifying copper electrolytes from a bismuth impurity on a laboratory scale. The electrolyte came from Polish copper electrorefineries with the content of main components, g/dm3: 49.6 Cu, 160 H2SO4. The electrolyte was enriched in bismuth by Bi2O3 addition. Purification of bismuth contamination was carried out using selected agents with adsorbing effects, such as barium hydroxide octahydrate, strontium carbonate, barium carbonate, barium and lead sulfates. The trials were performed until achieving the Bi level—below 0.1 g/dm3. During the experiments, it was noticed that electrolyte purification degree depends on initial Bi concentration in electrolyte, time and temperature, as well as on the type and amount of the bismuth-lowering agent. The most satisfactory results of Bi impurity removal were with additions of barium hydroxide octahydrate, strontium carbonate and barium carbonate to electrolyte at 60 °C for 1 h. These parameters revealed the highest electrolyte purification degree. Bismuth is not removed effectively from electrolytes by barium sulfate or lead sulfate addition. The efficiency of the purification process is much higher when the agents are added to the solution in the form of carbonates or hydroxides. Extending the electrolyte purification process time may cause dissolution of bismuth from the resulting precipitate and increase of bismuth concentration in electrolytes.

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