Introduction:
Wutou decoction has been wildly applied for the treatment of RA in China for
several thousand years.
Methods:
This study aims to develop a highly sensitive and specific ultra performance liquid chromatography
coupled with tandem mass spectrometry and electrospray ionization (UPLC-ESI-MS/MS)
method to explore the pharmacokinetic properties of three representative bioactive alkaloids after intragastric
administration of Wutou decoction in rats. Chromatographic separation was performed on a C18
column under the Multiple Reaction Monitoring (MRM) in the positive electrospray ionization (ESI)
mode. The pharmacokinetic parameters were evaluated by software DAS 3. 0.
Results:
The validation of the method was achieved in accordance with the FDA guidelines. The results
of pharmacokinetic study showed that the in vivo concentrations of benzoylmesaconine and
benzoylhypaconine were significantly higher than benzoylaconine. Our PK results showed that these
three compounds were quickly absorbed after the administration of Wutou decoction, and Tmax ranged
from 30 min to 45 min.
Conclusion:
The results of pharmacokinetic study showed that the in vivo concentrations of
benzoylmesaconine and benzoylhypaconine were significantly higher than benzoylaconine. There were
also similar pharmacokinetic behaviors observed among BAC, BHA, and BMA after oral administration
of WTD.
A Highly Sensitive and Rugged LC-MS/MS Method Development and Validation for Determination of Elvitegravir in Rat Plasma: Application to Pharmacokinetic Study