HPLC Determination of Fexofenadine in Human Plasma For Therapeutic Drug Monitoring and Pharmacokinetic Studies

2015 ◽  
Vol 30 (7) ◽  
pp. 1059-1064 ◽  
Author(s):  
S. A. Helmy ◽  
H. M. El Bedaiwy
Keyword(s):  
Therapeutic Drug Monitoring ◽  
Human Plasma ◽  
Drug Monitoring ◽  
Hplc Determination ◽  
Therapeutic Drug ◽  
Pharmacokinetic Studies

Simple HPLC Method for Quantitative Determination of Carbinoxamine in Human Plasma: Application in Pharmacokinetic Studies and Therapeutic Drug Monitoring

Drug Research ◽  
2019 ◽  
Vol 69 (06) ◽  
pp. 342-347
Author(s):  
Soha M. El-Masry ◽  
Rasha M. El-Morsi
Keyword(s):  
Therapeutic Drug Monitoring ◽  
Quantitative Determination ◽  
Human Plasma ◽  
Drug Monitoring ◽  
Hplc Method ◽  
Therapeutic Drug ◽  
Pharmacokinetic Studies ◽  
Simple Liquid ◽  
Limit Of Quantitation

AbstractA sensitive, specific and cost-effective HPLC method was developed for quantitative determination of carbinoxamine in human plasma using liquid chromatography with ultraviolet detection. A simple liquid–liquid extraction by ethyl acetate was used to extract carbinoxamine from human plasma. Satisfactory separation was achieved by a mobile phase consisting of 20 mM ammonium dihydrogen orthophosphate containing 0.01% triethyl amine (pH adjusted to 3 by using phosphoric acid) and acetonitrile in ratio of (75:25, v/v) at a flow rate of 0.9 mL/min on Hypersil® BDS C18 column (250×4.6 mm, 5 μm) column. The UV detector was set at 260 nm. The developed method was validated in human plasma with a lower limit of quantitation of 5 ng/mL for carbinoxamine. Linearity was demonstrated over the concentration range 5–200 ng/mL. The observed within- and between-day assay precision ranged from 1.902 to 14.90%; accuracy varied between 98.87 and 108.0%. This method can be used suitably for pharmacokinetic studies and in therapeutic drug monitoring in patients treated with carbinoxamine.

A simple and automated online SPE-LC-MS/MS method for simultaneous determination of olanzapine, fluoxetine and norfluoxetine in human plasma and its application in therapeutic drug monitoring

RSC Advances ◽  
2015 ◽  
Vol 5 (43) ◽  
pp. 34342-34352 ◽  
Author(s):  
Yiran Fan ◽  
Guanghu Shen ◽  
Ping Li ◽  
Xiaonan Xi ◽  
Haiting Wu ◽  
...  
Keyword(s):  
Therapeutic Drug Monitoring ◽  
Simultaneous Determination ◽  
Human Plasma ◽  
Drug Monitoring ◽  
Sample Pretreatment ◽  
Therapeutic Drug ◽  
High Quality ◽  
Online Spe

An integration of sample pretreatment automation using online SPE technique could provide an easy to use, efficient, sensitive and high quality methods for TDM.

Developing an isotope dilution UHPLC–MS/MS method to quantify linezolid in human plasma: application to therapeutic drug monitoring

Bioanalysis ◽  
2020 ◽  
Vol 12 (14) ◽  
pp. 991-1001
Author(s):  
Yanping Guan ◽  
Xiaoxia Yu ◽  
Ying Wang ◽  
Qinhai Li ◽  
Dan Liang ◽  
...  
Keyword(s):  
Therapeutic Drug Monitoring ◽  
Human Plasma ◽  
Drug Monitoring ◽  
Drug Exposure ◽  
Therapeutic Drug ◽  
The Us ◽  
Dosing Regimens ◽  
Us Fda ◽  
Isocratic Mode

Aim: To optimize clinical efficacy and reduce the drug-exposure-related toxicity of linezolid, whose concentrations show wide inter-variabilities, a simple and reliable quantitative assay for therapeutic drug monitoring is necessary. Results: A UHPLC–MS/MS assay has been established for determination of linezolid in human plasma and fully validated according to the US FDA guidelines. After a simple, isotope-dilluted precipitation with methanol, the analytes were separated by a straightforward isocratic mode and the MS/MS was conducted under the ESI+ mode fitted with SRM. The calibration curves proved acceptable linearity in the range of 0.1–30.0 µg/ml. Conclusion: The present assay is currently used in routine clinical practice, being applied to therapeutic drug monitoring and helps to optimize individual dosing regimens and manage adverse effects in ICU patients.

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