A Minimized Chemoenzymatic Cascade for Bacterial Luciferase in Bioreporter Applications

ChemBioChem ◽  
2020 ◽  
Vol 21 (14) ◽  
pp. 2073-2079 ◽  
Author(s):  
Jittima Phonbuppha ◽  
Ruchanok Tinikul ◽  
Thanyaporn Wongnate ◽  
Pattarawan Intasian ◽  
Frank Hollmann ◽  
...  
Keyword(s):  
Bacterial Luciferase

KINETIC AND MECHANISTIC INVESTIGATION OF THE BACTERIAL LUCIFERASE REACTION

1991 ◽  
pp. 273-276
Author(s):  
Frank M. Raushel ◽  
Husam M. Abu-Soud ◽  
Leisha S. Mullins ◽  
Wilson A. Francisco ◽  
Thomas O. Baldwin
Keyword(s):  
Bacterial Luciferase ◽  
Mechanistic Investigation

scholarly journals Bacterial luciferase subunits are synthesized in equal quantities.

1977 ◽  
Vol 252 (2) ◽  
pp. 803-805
Author(s):  
S C Tu ◽  
J C Makemson ◽  
J E Becvar ◽  
J W Hastings
Keyword(s):  
Bacterial Luciferase

scholarly journals Enhanced brightness of bacterial luciferase by bioluminescence resonance energy transfer

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Tomomi Kaku ◽  
Kazunori Sugiura ◽  
Tetsuyuki Entani ◽  
Kenji Osabe ◽  
Takeharu Nagai
Keyword(s):  
Energy Transfer ◽  
Mammalian Cells ◽  
Fluorescent Protein ◽  
Color Change ◽  
Yellow Fluorescent Protein ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Bioluminescence Resonance Energy Transfer ◽  
Bacterial Luciferase ◽  
Bacterial Bioluminescence

AbstractUsing the lux operon (luxCDABE) of bacterial bioluminescence system as an autonomous luminous reporter has been demonstrated in bacteria, plant and mammalian cells. However, applications of bacterial bioluminescence-based imaging have been limited because of its low brightness. Here, we engineered the bacterial luciferase (heterodimer of luxA and luxB) by fusion with Venus, a bright variant of yellow fluorescent protein, to induce bioluminescence resonance energy transfer (BRET). By using decanal as an externally added substrate, color change and ten-times enhancement of brightness was achieved in Escherichia coli when circularly permuted Venus was fused to the C-terminus of luxB. Expression of the Venus-fused luciferase in human embryonic kidney cell lines (HEK293T) or in Nicotiana benthamiana leaves together with the substrate biosynthesis-related genes (luxC, luxD and luxE) enhanced the autonomous bioluminescence. We believe the improved luciferase will forge the way towards the potential development of autobioluminescent reporter system allowing spatiotemporal imaging in live cells.

Structure and Catalytic Inactivity of the Bacterial Luciferase Neutral Flavin Radical

1982 ◽  
Vol 123 (2) ◽  
pp. 355-361 ◽  
Author(s):  
Manfred KURFURST ◽  
Sandro GHISLA ◽  
Robert PRESSWOOD ◽  
J. Woodland HASTTNGS
Keyword(s):  
Bacterial Luciferase

scholarly journals The Purification, Properties, and Chemiluminescent Quantum Yield of Bacterial Luciferase

1965 ◽  
Vol 240 (3) ◽  
pp. 1473-1481 ◽  
Author(s):  
J. Woodland Hastings ◽  
William H. Riley ◽  
Joseph Massa
Keyword(s):  
Quantum Yield ◽  
Bacterial Luciferase

scholarly journals The 9H-Fluoren Vinyl Ether Derivative SAM461 Inhibits Bacterial Luciferase Activity and Protects Artemia franciscana From Luminescent Vibriosis

2018 ◽  
Vol 8 ◽  
Author(s):  
Alberto J. Martín-Rodríguez ◽  
Sergio J. Álvarez-Méndez ◽  
Caroline Overå ◽  
Kartik Baruah ◽  
Tânia Margarida Lourenço ◽  
...  
Keyword(s):  
Vinyl Ether ◽  
Luciferase Activity ◽  
Artemia Franciscana ◽  
Bacterial Luciferase ◽  
Ether Derivative

Photokinetic assay of pyruvate in the islets of langerhans using bacterial luciferase

1977 ◽  
Vol 78 (1) ◽  
pp. 229-234 ◽  
Author(s):  
Ambjörn Ågren ◽  
Christian Berne ◽  
Sven E. Brolin
Keyword(s):  
Islets Of Langerhans ◽  
Bacterial Luciferase

Reversible steps in the reaction of aldehydes with bacterial luciferase intermediates

1979 ◽  
Vol 193 (2) ◽  
pp. 449-455 ◽  
Author(s):  
A.L. Baumstark ◽  
T.W. Cline ◽  
J.W. Hastings
Keyword(s):  
Bacterial Luciferase

Use ofEscherichia coli cloned with genes encoding bacterial luciferase for evaluation of chemical toxicity

1990 ◽  
Vol 5 (4) ◽  
pp. 337-350 ◽  
Author(s):  
Jorma Lampinen ◽  
Matti Korpela ◽  
Petri Saviranta ◽  
Rolf Kroneld ◽  
Matti Karp
Keyword(s):  
Ofescherichia Coli ◽  
Chemical Toxicity ◽  
Bacterial Luciferase ◽  
Genes Encoding
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