Immunocytochemical localization of opsin, visual arrestin, myosin III, and calmodulin inLimulus lateral eye retinular cells and ventral photoreceptors

2001 ◽  
Vol 435 (2) ◽  
pp. 211-225 ◽  
Author(s):  
Barbara-Anne Battelle ◽  
Alain Dabdoub ◽  
Michael A. Malone ◽  
Anne W. Andrews ◽  
Chelsi Cacciatore ◽  
...  
Keyword(s):  
Immunocytochemical Localization ◽  
Lateral Eye ◽  
Retinular Cells

The effect of manganous chloride and tetrodotoxin on Limulus lateral eye retinular cells

1973 ◽  
Vol 13 (12) ◽  
pp. 2327-2333 ◽  
Author(s):  
V.J. Wulff ◽  
Carlos Mendez
Keyword(s):  
Lateral Eye ◽  
Retinular Cells

A study of light-initiated currents in Limulus lateral eye retinular cells

1977 ◽  
Vol 2 (2) ◽  
pp. 113-121 ◽  
Author(s):  
V.J. Wulff ◽  
W.J. Mueller ◽  
J.L. Fahy
Keyword(s):  
Lateral Eye ◽  
Retinular Cells

The effect of sodium, potassium and calcium on Limulus lateral eye retinular cells

1973 ◽  
Vol 13 (12) ◽  
pp. 2309-2326 ◽  
Author(s):  
V.J. Wulff
Keyword(s):  
Sodium Potassium ◽  
Lateral Eye ◽  
Retinular Cells

The effect of cyclic AMP and aminophylline on Limulus lateral eye retinular cells

1973 ◽  
Vol 13 (12) ◽  
pp. 2335-2344 ◽  
Author(s):  
V.J. Wulff
Keyword(s):  
Cyclic Amp ◽  
Lateral Eye ◽  
Retinular Cells

The effect of light-dark adaptation on the ultrastructure ofLimulus lateral eye retinular cells

1976 ◽  
Vol 107 (1) ◽  
pp. 77-96 ◽  
Author(s):  
Mildred Behrens ◽  
Wolf Krebs
Keyword(s):  
Dark Adaptation ◽  
Lateral Eye ◽  
Retinular Cells ◽  
Effect Of Light

scholarly journals CELL JUNCTIONS IN OMMATIDIA OF LIMULUS

1967 ◽  
Vol 33 (2) ◽  
pp. 365-383 ◽  
Author(s):  
Arnaldo Lasansky
Keyword(s):  
Structural Features ◽  
Cell Junctions ◽  
Retinular Cell ◽  
Visual Cells ◽  
Lateral Eye ◽  
Distal Process ◽  
Retinular Cells ◽  
Intercellular Relationships ◽  
Ommatidial Cells

The intercellular relationships in the ommatidia of the lateral eye of Limulus have been investigated. The distal process of the eccentric cell gives origin to microvilli which interdigitate with the microvilli of the retinular cells. Therefore, both types of visual cells contribute to form the rhabdom and may have an analogous photoreceptor function. Quintuple-layered junctions are found within the rhabdom at the lines of demarcation between adjoining microvilli, whether the microvilli originate from a single retinular cell, from two adjacent retinular cells, or from a retinular cell and the eccentric cell. Furthermore, quintuple-layered junctions between the eccentric cell and the tips of the microvilli of the retinular cells occur at the boundary between the distal process and the rhabdom. These findings are interpreted to indicate that the rhabdom provides an extensive electrotonic junction relating retinular cells to one another and to the eccentric cell. Quintuple-layered junctions between glial and visual cells, as well as other structural features of the ommatidial cells, are also described.

The effect of cyclic AMP on Limulus lateral eye retinular cells

1971 ◽  
Vol 11 (12) ◽  
pp. 1493-1495 ◽  
Author(s):  
V.J. Wulff
Keyword(s):  
Cyclic Amp ◽  
Lateral Eye ◽  
Retinular Cells

Photoreceptor cells dissociated from the compound lateral eye of the horseshoe crab, Limulus polyphemus, I: Structure and ultrastructure

1993 ◽  
Vol 10 (4) ◽  
pp. 597-607 ◽  
Author(s):  
Robert N. Jinks ◽  
W. J. Brad Hanna ◽  
George H. Renninger ◽  
Steven C. Chamberlain
Keyword(s):  
Horseshoe Crab ◽  
Photoreceptor Cells ◽  
Normal Structure ◽  
Limulus Polyphemus ◽  
Whole Cell ◽  
Enzyme Pretreatment ◽  
Essentially Normal ◽  
Lateral Eye ◽  
Retinular Cells ◽  
Structure And Ultrastructure

AbstractIsolated photoreceptors are desirable for whole-cell and patch-clamp studies of functional properties of visual processes that cannot be clearly analyzed when the photoreceptors are coupled. The retina of the compound lateral eye of the horseshoe crab, Limulus polyphemus, was dissociated into individual retinular cells using an enzyme pretreatment consisting of collagenase, papain, and trypsin, and a two-stage mechanical dissociation. These photoreceptors are functionally viable in an organ culture medium for up to 1 week and possess naked arhabdomeral and rhabdomeral segment membranes which are easily accessible for whole-cell recordings. A dissection technique was also developed whereby the retinal epidermis and neural plexus, as well as the second-order eccentric cells, could be separated from the ommatidia of the compound lateral eye in one simple step, providing viable isolated ommatidia attached to the cornea. The enzyme pretreatment used for dissociating the retina was then used to remove the individual ommatidia from the corneal cones.Hoffman modulation contrast microscopy was used to develop a reliable method for sorting and collecting viable isolated retinular cells for morphological and electrophysiological studies. Morphological analysis using light microscopy and scanning and transmission electron microscopy revealed that isolated retinular cells are morphologically nearly identical to retinular cells in situ. Isolated retinular cells possess a normal rhabdomere with no apparent loss of microvillar membrane as a result of the isolation process. Ommatidia can presently be isolated with up to six retinular cells possessing essentially normal structure and ultrastructure including thick rays of rhabdom. Isolated ommatidia possess naked A-segment membranes which are also well suited for whole-cell recording techniques.

Dark adaptation and sodium pump activity in Limulus lateral eye retinular cells

1975 ◽  
Vol 15 (7) ◽  
pp. 759-765 ◽  
Author(s):  
V.J. Wulff ◽  
H. Steve ◽  
J.L. Fahy
Keyword(s):  
Dark Adaptation ◽  
Sodium Pump ◽  
Lateral Eye ◽  
Pump Activity ◽  
Retinular Cells

Immunocytochemical localization of p65 with one-nanometer gold particles following silver development

1989 ◽  
Vol 47 ◽  
pp. 1042-1043
Author(s):  
Richard W. Burry ◽  
Diane M. Hayes
Keyword(s):  
Plasma Membrane ◽  
Bovine Serum Albumin ◽  
Calf Serum ◽  
Specific Protein ◽  
Immunocytochemical Localization ◽  
Electron Microscopic ◽  
Gold Particles ◽  
Pass Through ◽  
Label Distribution ◽  
Phosphate Buffered Saline

Electron microscopic (EM) immunocytochemistry localization of the neuron specific protein p65 could show which organelles contain this antigen. Antibodies (Ab) labeled with horseradish peroxidase (HRP) followed by chromogen development show a broad diffuse label distribution within cells and restricting identification of organelles. Particulate label (e.g. 10 nm colloidal gold) is highly desirable but not practical because penetration into cells requires destroying the plasma membrane. We report pre-embedding immunocytochemistry with a particulate marker, 1 nm gold, that will pass through membranes treated with saponin, a mild detergent.Cell cultures of the rat cerebellum were fixed in buffered 4% paraformaldehyde and 0.1% glutaraldehyde (Glut.). The buffer for all incubations and rinses was phosphate buffered saline with: 1% calf serum, 0.2% saponin, 0.1% gelatin, 50 mM glycine 1 mg/ml bovine serum albumin, and (not in the HRP labeled cultures) 0.02% sodium azide. The monoclonal #48 to p65 was used with three label systems: HRP, 1 nm avidin gold with IntenSE M development, and 1 nm avidin gold with Danscher development.

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