Front Cover: Nano‐Optical Tweezers: Methods and Applications for Trapping Single Molecules and Nanoparticles (ChemPhysChem 14/2021)

Joshua D. Kolbow; Nathan C. Lindquist; Christopher T. Ertsgaard; Daehan Yoo; Sang‐Hyun Oh

doi:10.1002/cphc.202100447

Front Cover: Nano‐Optical Tweezers: Methods and Applications for Trapping Single Molecules and Nanoparticles (ChemPhysChem 14/2021)

ChemPhysChem ◽

10.1002/cphc.202100447 ◽

2021 ◽

Vol 22 (14) ◽

pp. 1407-1407

Author(s):

Joshua D. Kolbow ◽

Nathan C. Lindquist ◽

Christopher T. Ertsgaard ◽

Daehan Yoo ◽

Sang‐Hyun Oh

Keyword(s):

Optical Tweezers ◽

Single Molecules ◽

Front Cover

Download Full-text

Nano‐Optical Tweezers: Methods and Applications for Trapping Single Molecules and Nanoparticles

ChemPhysChem ◽

10.1002/cphc.202100441 ◽

2021 ◽

Vol 22 (14) ◽

pp. 1408-1408

Author(s):

Joshua D. Kolbow ◽

Nathan C. Lindquist ◽

Christopher T. Ertsgaard ◽

Daehan Yoo ◽

Sang‐Hyun Oh

Keyword(s):

Optical Tweezers ◽

Single Molecules

Download Full-text

Micromanipulation by laser microbeam and optical tweezers: from plant cells to single molecules

Journal of Microscopy ◽

10.1046/j.1365-2818.2000.00698.x ◽

2000 ◽

Vol 198 (3) ◽

pp. 182-187 ◽

Cited By ~ 45

Author(s):

K. O. Greulich ◽

G. Pilarczyk ◽

A. Hoffmann ◽

G. Meyer Zu Horste ◽

B. Schafer ◽

...

Keyword(s):

Optical Tweezers ◽

Single Molecules ◽

Plant Cells ◽

Laser Microbeam

Download Full-text

New tools to study biophysical properties of single molecules and single cells

Anais da Academia Brasileira de Ciências ◽

10.1590/s0001-37652007000100003 ◽

2007 ◽

Vol 79 (1) ◽

pp. 17-28 ◽

Cited By ~ 7

Author(s):

Márcio S. Rocha ◽

Oscar N. Mesquita

Keyword(s):

Optical Tweezers ◽

Osmotic Shock ◽

Single Cells ◽

Single Molecules ◽

Numerical Aperture ◽

Single Individual ◽

Biophysical Properties ◽

Individual Object ◽

Transparent Objects ◽

Volume Measurements

We present a review on two new tools to study biophysical properties of single molecules and single cells. A laser incident through a high numerical aperture microscope objective can trap small dielectric particles near the focus. This arrangement is named optical tweezers. This technique has the advantage to permit manipulation of a single individual object. We use optical tweezers to measure the entropic elasticity of a single DNA molecule and its interaction with the drug Psoralen. Optical tweezers are also used to hold a kidney cell MDCK away from the substrate to allow precise volume measurements of this single cell during an osmotic shock. This procedure allows us to obtain information about membrane water permeability and regulatory volume increase. Defocusing microscopy is a recent technique invented in our laboratory, which allows the observation of transparent objects, by simply defocusing the microscope in a controlled way. Our physical model of a defocused microscope shows that the image contrast observed in this case is proportional to the defocus distance and to the curvature of the transparent object. Defocusing microscopy is very useful to study motility and mechanical properties of cells. We show here the application of defocusing microscopy to measurements of macrophage surface fluctuations and their influence on phagocytosis.

Download Full-text

Optical manipulation of single molecules in the living cell

Physical Chemistry Chemical Physics ◽

10.1039/c4cp00208c ◽

2014 ◽

Vol 16 (25) ◽

pp. 12614-12624 ◽

Cited By ~ 27

Author(s):

Kamilla Norregaard ◽

Liselotte Jauffred ◽

Kirstine Berg-Sørensen ◽

Lene B. Oddershede

Keyword(s):

Optical Tweezers ◽

Living Cell ◽

Single Molecules ◽

Optical Manipulation ◽

Force Measurements

Optical tweezers are the only nano-tools capable of manipulating and performing force-measurements on individual molecules and organelles inside the living cell. We present methodologies for in vivo calibration and exciting recent results.

Download Full-text