Genomic DNA Isolation from Maize ( Zea mays ) Leaves Using a Simple, High‐Throughput Protocol

2016 ◽  
Vol 1 (1) ◽  
pp. 15-27 ◽  
Author(s):  
Kristen A. Leach ◽  
Paula C. McSteen ◽  
David M. Braun
Keyword(s):  
Zea Mays ◽  
High Throughput ◽  
Genomic Dna ◽  
Dna Isolation ◽  
Genomic Dna Isolation

A Low-Cost High-Throughput Method for Plant Genomic DNA Isolation

2019 ◽  
pp. 1-7
Author(s):  
Prateek Gupta ◽  
Hymavathi Salava ◽  
Yellamaraju Sreelakshmi ◽  
Rameshwar Sharma
Keyword(s):  
High Throughput ◽  
Genomic Dna ◽  
Dna Isolation ◽  
Low Cost ◽  
Genomic Dna Isolation ◽  
High Throughput Method

An efficient, simple and high throughput protocol for cotton genomic DNA isolation

2016 ◽  
Vol 25 (4) ◽  
pp. 437-441 ◽  
Author(s):  
Bipinchandra B. Kalbande ◽  
Anita S. Patil ◽  
Pranjib K. Chakrabarty
Keyword(s):  
High Throughput ◽  
Genomic Dna ◽  
Dna Isolation ◽  
Genomic Dna Isolation

scholarly journals Genomic DNA isolation from scrophularieae dried leaves using a simple, high-throughput protocol

2019 ◽  
Vol 48 (4) ◽  
pp. 1231-1235
Author(s):  
Mehrshid Riahi ◽  
Melina Babaei ◽  
Farrokh Ghahremaninejad
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
Dna Extraction ◽  
High Throughput ◽  
Genomic Dna ◽  
Dna Isolation ◽  
High Molecular Weight Dna ◽  
Plant Dna ◽  
Genomic Dna Isolation

This communication described efficient DNA extraction from Scrophularia and Verbascum samples. Modified Murray and Thompson modified Cota-Sànchez method and Bioflux kit methods were applied for the extraction of DNA. Among the different methods, Bioflux kit Plant DNA extraction kit, coupled with some modification was the best for extraction of high molecular weight DNA as long as the extracted DNA is quantified with fluorescence-based methods.

Genomic DNA Isolation, Amplification of the D1S80 Locus and DNA-Gel Electrophoresis

2015 ◽  
pp. 42-42
Author(s):  
Arti Pandey ◽  
Arun Pandey ◽  
Naveen Shreevastava ◽  
Durga Neupane
Keyword(s):  
Gel Electrophoresis ◽  
Genomic Dna ◽  
Dna Isolation ◽  
Genomic Dna Isolation ◽  
D1s80 Locus

scholarly journals Chemically synthesized silver nanoparticles as cell lysis agent for bacterial genomic DNA isolation

2017 ◽  
Vol 8 (4) ◽  
pp. 045015 ◽  
Author(s):  
Gunajit Goswami ◽  
Himangshu Boruah ◽  
Trishnamoni Gautom ◽  
Dibya Jyoti Hazarika ◽  
Madhumita Barooah ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Genomic Dna ◽  
Dna Isolation ◽  
Cell Lysis ◽  
Genomic Dna Isolation

An effective protocol to solve the problem in genomic DNA isolation of tung tree

2013 ◽  
Vol 22 (4) ◽  
pp. 492-497 ◽  
Author(s):  
Lingling Zhang ◽  
Lijing Dai ◽  
Junbo Gou ◽  
Junhua Peng
Keyword(s):  
Genomic Dna ◽  
Dna Isolation ◽  
Tung Tree ◽  
Genomic Dna Isolation

Rapid and High Quality DNA Isolation from Origanum onites for RAPD and ISSR Analysis

2008 ◽  
Vol 63 (7-8) ◽  
pp. 595-598 ◽  
Author(s):  
Emel Sözen ◽  
Ismail Poyraz
Keyword(s):  
Genomic Dna ◽  
Dna Isolation ◽  
Leaf Tissue ◽  
Isoamyl Alcohol ◽  
Molecular Study ◽  
Inter Simple Sequence Repeat ◽  
Limiting Factor ◽  
Hexadecyltrimethylammonium Bromide ◽  
Genomic Dna Isolation ◽  
Origanum Onites

Origanum onites is an economically important medicinal plant with high essential oil content. Lack of an appropriate DNA isolation procedure is a limiting factor for any molecular study of this plant. We have used a protocol for genomic DNA isolation based on a hexadecyltrimethylammonium bromide (CTAB) method described for other plant species. The method involves mortar grinding of leaf tissue, modified CTAB extraction using high salt concentrations and polyvinyl pyrrolidone, and successive isoamyl alcohol/chloroform extractions. The yield was approx. 20 μg DNA per 200 mg of initial fresh plant material. The genomic DNA obtained by this method was suitable to be used in restriction digests, inter simple sequence repeat (ISSR) and randomly amplified polymorphic DNA (RAPD) reactions. This extraction method should facilitate the molecular analysis of Origanum chemotypes.

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