scholarly journals Differentiation Protocol for 3D Retinal Organoids, Immunostaining and Signal Quantitation

2020 ◽  
Vol 55 (1) ◽  
Author(s):  
Hannah Döpper ◽  
Julia Menges ◽  
Morgane Bozet ◽  
Alexandra Brenzel ◽  
Dietmar Lohmann ◽  
...  
Keyword(s):  
Differentiation Protocol

scholarly journals Resveratrol Ameliorates the Maturation Process of β-Cell-Like Cells Obtained from an Optimized Differentiation Protocol of Human Embryonic Stem Cells

PLoS ONE ◽  
2015 ◽  
Vol 10 (3) ◽  
pp. e0119904 ◽  
Author(s):  
Daniela Pezzolla ◽  
Javier López-Beas ◽  
Christian C. Lachaud ◽  
Alejandro Domínguez-Rodríguez ◽  
Tarik Smani ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Maturation Process ◽  
Β Cell ◽  
Differentiation Protocol

scholarly journals Tenogenic differentiation protocol in xenogenic-free media enhances tendon-related marker expression in ASCs

PLoS ONE ◽  
2019 ◽  
Vol 14 (2) ◽  
pp. e0212192 ◽  
Author(s):  
Deborah Stanco ◽  
Christian Caprara ◽  
Gianluca Ciardelli ◽  
Luca Mariotta ◽  
Mauro Gola ◽  
...  
Keyword(s):  
Tenogenic Differentiation ◽  
Differentiation Protocol ◽  
Marker Expression ◽  
Free Media

P4023 Cloned horses: MtDNA heteroplasmy makes difficult the differentiation protocol

2016 ◽  
Vol 94 (suppl_4) ◽  
pp. 90-90
Author(s):  
M. Costa ◽  
B. Elguero ◽  
C. Ratti ◽  
M. Martinez
Keyword(s):  
Differentiation Protocol

scholarly journals An In Vitro Differentiation Protocol for Human Embryonic Bipotential Gonad and Testis Cell Development

2020 ◽  
Vol 15 (6) ◽  
pp. 1377-1391
Author(s):  
Ingrid M. Knarston ◽  
Svenja Pachernegg ◽  
Gorjana Robevska ◽  
Irene Ghobrial ◽  
Pei Xuan Er ◽  
...  
Keyword(s):  
Cell Development ◽  
In Vitro Differentiation ◽  
Differentiation Protocol ◽  
Bipotential Gonad

scholarly journals Do not keep it simple: recent advances in the generation of complex organoids

2020 ◽  
Vol 127 (11) ◽  
pp. 1569-1577 ◽  
Author(s):  
Philipp Wörsdörfer ◽  
Takashi I ◽  
Izumi Asahina ◽  
Yoshinori Sumita ◽  
Süleyman Ergün
Keyword(s):  
Stem Cell ◽  
3D Cell Culture ◽  
Adult Stem Cell ◽  
Inflammatory Cells ◽  
Stem Cell Population ◽  
Special Focus ◽  
Differentiation Protocol ◽  
Organ Specific ◽  
Culture Models

Abstract 3D cell culture models which closely resemble real human tissues are of high interest for disease modelling, drug screening as well as a deeper understanding of human developmental biology. Such structures are termed organoids. Within the last years, several human organoid models were described. These are usually stem cell derived, arise by self-organization, mimic mechanisms of normal tissue development, show typical organ morphogenesis and recapitulate at least some organ specific functions. Many tissues have been reproduced in vitro such as gut, liver, lung, kidney and brain. The resulting entities can be either derived from an adult stem cell population, or generated from pluripotent stem cells using a specific differentiation protocol. However, many organoid models only recapitulate the organs parenchyma but are devoid of stromal components such as blood vessels, connective tissue and inflammatory cells. Recent studies show that the incorporation of endothelial and mesenchymal cells into organoids improved their maturation and might be required to create fully functional micro-tissues, which will allow deeper insights into human embryogenesis as well as disease development and progression. In this review article, we will summarize and discuss recent works trying to incorporate stromal components into organoids, with a special focus on neural organoid models.

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