Journal of Animal Science
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Published By Oxford University Press

1525-3163, 0021-8812
Updated Tuesday, 14 September 2021

Author(s):  
Nicolas I Busse ◽  
Madison L Gonzalez ◽  
Mackenzie L Krason ◽  
Sally E Johnson

Abstract Consumption of β-hydroxy β-methylbutyrate (HMB) alters muscle composition and metabolism leading to strength and agility improvements in human athletes. To determine if HMB affects athletic performance and muscle function in horses, Thoroughbred geldings were fed a control (CON; n=5) or HMB (n=6) supplement for 6 wks prior to completing a standardized exercise test (SET). Gluteus Medius (GM) muscle biopsies were obtained before the SET for fiber typing. Heart rate (HR), biceps femoris (BF) and semitendinosus (ST) surface electromyograms (EMG) and fore and hind limb metacarpophalangeal joint angles were captured at the gallop of the SET. Results demonstrate that HMB supplementation increased (P < 0.05) the percentage of type IIA and IIA/X muscle fibers in the GM with a corresponding decrease (P < 0.05) in type IIX fibers. The percentage of type I fibers was unaffected by diet. Supplementation with HMB did not result in any measurable effects on performance or biomechanical properties by comparison to CON. Supplementation with HMB resulted in an increase (P < 0.05) in ST median frequency at speeds of 10 m/s and greater. Increasing treadmill speed resulted in an increase (P < 0.05) in stride length and the maximal proximal forelimb fetlock angle, and a decrease (P < 0.05) in stance phase time of the gait cycle. Integrated EMG increased (P < 0.05) with increasing treadmill speeds for both the BF and ST with the BF exhibiting greater (P < 0.05) iEMG values than the ST. In summary, HMB increased the percentage of type IIA GM fibers which did not translate into improved performance.


Author(s):  
MaryAnn J Matney ◽  
Morgan E Gravely ◽  
Travis G O’Quinn ◽  
James S Drouillard ◽  
Kelsey J Phelps-Ronningen ◽  
...  

Abstract The objective of this study was to determine effects of extended aging and intramuscular location on Warner-Bratzler shear force (WBSF), muscle fiber cross-sectional area (CSA), and protein degradation of semitendinosus (ST) and longissimus lumborum (LL) steaks. Left ST and LL were removed from 40 carcasses at 6 d postmortem. The ST was fabricated into 5 locations (LOC), with LOC 1 being most proximal and LOC 5 being most distal. The posterior LL was fabricated into 3 LOC, with LOC 1 being most anterior. Vacuum sealed ST steaks were aged 7, 14, 28, 56, or 112 d postmortem, while LL steaks were aged 7, 28, or 112 d postmortem at 2 ± 1°C. A steak from each LOC was assigned to WBSF or laboratory analyses. There were no Day of Aging (DOA) × LOC interactions for all dependent variables (P > 0.06). There were DOA effects for ST and LL WBSF values and degraded 38-kDa desmin (DES; P < 0.01). Day-7 ST-steak WBSF value was greater than all other days (P < 0.01) and d-14 steaks had greater WBSF value than remaining days (P < 0.05). Day-28 ST-steak WBSF values were greater than d-56 and -112 (P < 0.01), which did not differ (P = 0.53). In the LL, d-7 steaks had greater WBSF values than the other two timepoints (P < 0.01) and d-28 steaks had greater (P < 0.01) WBSF values than d-112 steaks. Degraded ST 38-kDa DES content was less on d 7 and 14 compared to all other days (P < 0.03), but did not differ (P = 0.79) from each other. Days 28 and 56 38-kDa DES content was less than d 112 (P < 0.01), but did not differ (P = 0.34) from each other. Degraded LL 38-kDa DES content was less on d 7 than d 28 and 112 (P < 0.02), which did not differ (P = 0.67). There were LOC effects for only ST WBSF and muscle fiber CSA (P < 0.05). Semitendinosus steak LOC 1 and 2 had greater WBSF values than all other locations (P < 0.01), but did not differ (P = 0.32) from each other. Semitendinosus steak LOC 3 and 5 had greater WBSF values than LOC 4 (P < 0.01), but did not differ (P = 0.85) from each other. The CSA of all ST fiber types were largest in LOC 1 compared to all other fiber types (P < 0.01). The CSA of all LOC 2 and 3 fiber types was greater than LOC 4 and 5 (P < 0.01), but were not different from each other (P > 0.81), and LOC 4 had greater CSA than LOC 5 (P < 0.01). Steak aging WBSF value improvements seemed proteolysis catalyzed, while the ST intramuscular tenderness gradient was more likely due to muscle fiber CSA.


Author(s):  
Luiz F Batista Dias ◽  
Aaron B Norris ◽  
Jordan M Adams ◽  
Thomas B Hairgrove ◽  
Luis O Tedeschi

Abstract Rumen acidosis is a common metabolic disorder occurring when organic acid production exceeds clearance capacity, reducing ruminal pH. The occurrence of acidosis has been directly correlated to the ratio of concentrate to forage in the diet. However, rates of substrate fermentation and acid absorption vary at different locations in the reticulo-rumen. The objective of this study was to determine the pH and redox potential (Eh) in different locations of the reticulo-rumen using 16 ruminally cannulated steers (309 ± 43 kg) receiving different supplementation levels of quebracho extract (QT; Schinopsis balansae) within a grower-type diet (CP : 13.4 %; TDN: 70.4 %, and ME : 2.55 Mcal/kg, basis dry matter [DM] basis). Animals were randomly assigned to one of four dietary treatments: QT at 0, 1, 2, and 3% of DM (QT0, QT1, QT2, and QT3; respectively), containing about 0, 0.7, 1.4, and 2.1% of condensed tannins (CT), DM basis, respectively. Animals were adapted to the basal diet for 12-d before being introduced to predetermined treatments for 4 weeks (wk), with diets provided twice daily to allow ad libitum intake. Weekly measurements of ruminal fluid pH and Eh were taken 4 h post-feeding using a portable pH meter with 2 probes (pH and redox) in four locations of the reticulo-rumen (reticulum, cranial sac, dorsal sac, and ventral sac). Data were analyzed using a random coefficients model with the pen as a random effect and wk as repeated measures, with DM intake included as a covariate. There was no interaction among treatments, location, and wk (P ≥ 0.882) on reticulo-ruminal pH. Overall, ruminal pH was lower for QT0 and QT1 compared to QT3 (P < 0.001). The pH in the reticulum was greater than those of the ventral and dorsal sacs (6.05 vs. 5.94, 5.89, respectively; P ≤ 0.001) but similar to cranial sac (6.00). Reticular pH was positively correlated with the ruminal locations ( ≥ 0.78; P < 0.001). The linear equation to estimate ruminal mean pH using reticulum pH had an intercept and slope different from zero (P ≤ 0.04), but CT (% DM) was not different from zero (P = 0.15), root mean square error of 0.15, and R 2 of 0.778: 0.723 (±0.36) + 0.857 (±0.059) × Reticulum pH + 0.033 (±0.023) × CT. The redox potential was lower for QT0 in wk 1 than all other treatments (P < 0.001). We concluded that reticulo-ruminal pH differs among locations in the rumen regardless of QT supplementation level and days on feed, with reticular pH being the highest.


Author(s):  
Yang Yun ◽  
Shuli Ji ◽  
Ge Yu ◽  
Peilu Jia ◽  
Yu Niu ◽  
...  

Abstract The present study used intrauterine growth restriction (IUGR) piglets as an animal model to determine the effect of Bacillus subtilis on intestinal integrity, antioxidant capacity, and microbiota in the jejunum of suckling piglets. In total, 8 normal birth weight (NBW) newborn piglets (1.62 ± 0.10 kg) and 16 newborn IUGR piglets (0.90 ± 0.08 kg) were selected and assigned to 3 groups. Piglets were orally gavaged with 10 mL sterile saline (NBW and IUGR groups), IUGR piglets were orally gavaged with 10 mL/d bacterial fluid (Bacillus subtilis diluted in sterile saline, gavage in the dose of 2 × 109 colony-forming units per kg of body weight) (IBS group) (n = 8). IUGR induced jejunal barrier dysfunction and redox status imbalance of piglets, and changed the abundances of bacteria in the jejunum. Treatment with Bacillus subtilis increased (P < 0.05) the ratio of villus height to crypt depth (VH/CD) in the jejunum, decreased (P < 0.05) the plasma diamine oxidase (DAO) activity, and enhanced (P < 0.05) the gene expressions of zonula occludens-1 (ZO-1), Occludin and Claudin-1 in the jejunum of IUGR piglets. Treatment with Bacillus subtilis decreased (P < 0.05) the concentration of protein carbonyl (PC), and increased (P < 0.05) the activities of catalase (CAT) and total superoxide dismutase (T-SOD) in the jejunum of IUGR piglets. Treatment with Bacillus subtilis also increased (P < 0.05) gene expressions of superoxide dismutase 1 (SOD1), CAT, and nuclear factor erythroid 2-related factor (Nrf2), as well as the protein expressions of heme oxygenase-1 (HO-1), SOD1, and Nrf2 in the jejunum of IUGR piglets. Treatment with Bacillus subtilis also improved the abundances and the community structure of bacteria in the jejunum of IUGR piglets. These results suggested that IUGR damaged the jejunal barrier function and antioxidant capacity of suckling piglets, altered the abundances of bacteria in the jejunum. Treatment with Bacillus subtilis improved the intestinal integrity and antioxidant capacity, while also improved the abundances and structure of bacteria in the jejunum of suckling piglets.


2021 ◽  
Vol 99 (9) ◽  
Author(s):  
Gilberto Solano-Suárez ◽  
Luciano S Caixeta ◽  
Alexander Masic ◽  
Diego Manríquez ◽  
Luciana Hatamoto-Zervoudakis ◽  
...  

Abstract The main objective of this study was to evaluate the effect of peripartal administration of a commercially available nonspecific immune stimulant (mycobacterium cell wall fraction; MCWF [Amplimune, NovaVive Inc., Napanee, ON, Canada]) on the incidence of disease during early lactation and subsequent fertility of dairy cows. A second objective was to characterize the dynamics of circulating white blood cells (WBC) and metabolic markers following treatment administration. Cows in an United States Department of Agriculture (USDA) organic-certified dairy herd were blocked by parity and, based on sequential calving dates, randomly assigned to receive two injections (5 mL s.c.) of either a placebo (saline solution) as a control (CON; n = 71) or MCWF (n = 65) at enrollment (7 d before expected calving) and within 24 h after calving. Blood samples were collected from a subsample of the study population (MCWF = 16; CON = 18) for WBC count at enrollment, at day 2 post enrollment, and at days 1, 3, 7, and 14 after calving. Serum fatty acids, beta-hydroxybutyrate, and Ca concentrations were determined at days 1 and 7 postpartum (MCWF = 21; CON = 21). Main outcome variables included incidence risk of peripartal and early lactation health disorders and pregnancy at first artificial insemination (AI), at 100, and at 150 days in milk (DIM). In addition, the average daily milk yield up to 90 DIM and death and live culling before 305 DIM were compared. Treatment effects were assessed using multivariable logistic regression, time-to-event analyses, and repeated measures analysis of variance (ANOVA). A treatment effect on the incidence risk of some of the health disorders in the study was established. Incidence risk of metritis and clinical mastitis <28 DIM was smaller in MCWF than in CON cows (36.9% vs. 50.7% and 6.3% vs. 19.7%, respectively). On the contrary, the incidence risk of respiratory disease <28 DIM was smaller in CON (0%) than in MCWF (7.7%). Reproductive performance of multiparous cows was affected by MCWF administration: pregnancy at first AI and pregnancy at 100 and 150 DIM were greater in MCWF than in CON (35.6% vs. 19.2%; 51.1% vs. 25.0%; and 64.4% vs. 40.4%, respectively). Overall, median intervals from calving to pregnancy were 90 vs. 121 d in MCWF and CON cows, respectively. No treatment effects on the dynamics of circulating WBC or in postpartum metabolic status were established. No differences for milk yield or for the proportion of cows that survived up to 305 DIM were determined, although cows in MCWF left the herd earlier than cows in CON. In conclusion, incidence risks of metritis and mastitis in early lactation were smaller in cows receiving MCWF, whereas the incidence risk of respiratory disease was smaller in CON. Fertility significantly improved in MCWF compared with CON cows. As this study was performed in an organic-certified dairy, specific health and reproductive management practices may affect the external validity of the current findings.


2021 ◽  
Vol 99 (9) ◽  
Author(s):  
Octavio Guimaraes ◽  
Sam Jalali ◽  
John J Wagner ◽  
Jerry W Spears ◽  
Terry Eugene Engle

Abstract Twelve Angus steers (BW 452.8 ± 6.1 kg) fitted with ruminal cannulae were used to determine the impact of trace mineral (TM) source on digestibility, ruminal volatile fatty acid (VFA) composition, ruminal soluble concentrations of Cu, Zn, and Mn, and relative binding strength of trace minerals located in the rumen insoluble digesta fraction. Steers were fed a medium-quality grass hay diet (DM basis: 10.8% CP, 63.1% neutral detergent fiber [NDF], 6.9 mg Cu/kg, 65.5 mg Mn/kg, and 39.4 mg Zn/kg) supplemented with protein for 21 d. Treatments consisted of either sulfate (STM) or hydroxy (HTM) sources (n = 6 steers/treatment) to provide 20, 40, and 60 mg supplemental Cu, Mn, and Zn/kg DM, respectively. Following a 21-d adaptation period, total fecal output was collected for 5 d. Dry matter (P < 0.07) and CP (P < 0.06) digestibility tended to be reduced, and NDF (P < 0.04) and acid detergent fiber (ADF) (P < 0.05) digestibility were reduced in STM- vs. HTM-supplemented steers. On day 6, ruminal fluid was collected at 0, 2, and 4 h post-feeding and analyzed for VFA. There were no treatment x time interactions for VFA. Steers receiving HTM had less (P < 0.02) molar proportions of butyric acid and greater (P < 0.05) total VFA concentrations than STM-supplemented steers. Steers were then fed the same diet without supplemental Cu, Zn, or Mn for 14 d. On day 15 steers received a pulse dose of 20 mg Cu, 40 mg Mn, and 60 mg Zn/kg DM from either STM or HTM (n = 6 steers/treatment). Ruminal samples were obtained at 2-h intervals starting at −4 and ending at 24 h relative to dosing. There was a treatment x time interaction (P < 0.03) for ruminal soluble Cu, Mn, and Zn concentrations. Ruminal soluble mineral concentrations were greater (P < 0.05) for Cu at 4, 6, 8, 10, 12, and 14 h; for Mn at 4 and 6 h; and for Zn at 4, 6, and 8 h post-dosing in STM compared with HTM-supplemented steers. Copper concentrations were greater (P < 0.05) at 12 and 24 h and Zn concentrations in ruminal solid digesta were greater at 24 h in HTM-supplemented steers. Upon dialysis against Tris-EDTA, the percent Zn released from digesta was greater (P < 0.05) at 12 h (P < 0.03) and 24 h (P < 0.05), and the percent Cu released was greater (P < 0.02) at 24 h post-dosing in HTM steers when compared with STM-supplemented steers. Results indicate that Cu and Zn from HTM have low solubility in the rumen and appear to be less tightly bound to ruminal solid digesta than Cu and Zn from STM. The lower ruminal soluble concentrations of Cu and Zn in steers given HTM were associated with greater fiber digestibility.


Author(s):  
Wade M Hutchens ◽  
Mike D Tokach ◽  
Steve S Dritz ◽  
Jordan Gebhardt ◽  
Jason C Woodworth ◽  
...  

Abstract This experiment was conducted to evaluate potential replacements for pharmacological levels of Zn (provided by Zn oxide), such as diet acidification (sodium diformate), and low dietary crude protein (CP: 21 vs 18%) on nursery pig performance and fecal dry matter (DM). A total of 360 weaned pigs (Line 200 × 400, DNA, Columbus, NE; initially 5.90 ± 0.014 kg) were used in a 42-d growth study. Pigs were weaned at approximately 21-d of age and randomly assigned to pens (5 pigs per pen). Pens were then allotted to 1 of 8 dietary treatments with 9 pens per treatment. Experimental diets were fed in two phases: Phase 1 from weaning to d 7 and phase 2 from d 7 to 21; with all pigs fed the same common diet from d 21 to 42. The eight treatment diets were arranged as a 2 × 2 × 2 factorial with main effects of Zn (110 mg/kg from d 0 to 21 or 3,000 mg/kg from d 0 to 7, and 2,000 mg/kg from d 7 to 21), diet acidification, (without or with 1.2% sodium diformate), and dietary CP (21 or 18%, 1.40 and 1.35% in Phase 1 and 2 vs 1.20% standardized ileal digestible Lys, respectively). Fecal samples were collected weekly from the same 3 pigs per pen to determine DM content. No 2- or 3-way interactions (P > 0.05) were observed throughout the 42-d study for growth performance; however, there was a Zn × acidifier × CP interaction (P < 0.05) for fecal DM on d 7 and for the overall average of the 6 collection periods. Reducing CP without acidification or pharmacological levels of Zn increased fecal DM, but CP had little effect when ZnO was present in the diet. From d 0 to 21, significant (P < 0.05) main effects were observed where average daily gain (ADG) and gain:feed (G:F) increased for pigs fed pharmacological levels of Zn, sodium diformate, or 21% CP (P < 0.065). In the subsequent period (d 21 to 42) after the experimental diets were fed, there was no evidence of difference in growth performance among treatments. Overall (d 0 to 42), main effect tendencies were observed (P < 0.066) for pigs fed added Zn or sodium diformate from d 0 to 21 whereas pigs fed 21% CP had greater G:F than those fed 18% CP. Pig weight on d 42 was increased by adding Zn (P < 0.05) or acidifier (P < 0.06) but not CP. In summary, none of the feed additives had a major influence on fecal DM, but dietary addition of pharmacological levels of Zn or sodium diformate independently improved nursery pig performance.


Author(s):  
Jing Zhou ◽  
Yan Qin ◽  
Xia Xiong ◽  
Zhaobin Wang ◽  
Min Wang ◽  
...  

Abstract This study aimed to investigate the effects of iron, vitamin A (VA) and their interaction on intestinal development and differentiation of cells in suckling piglets. Therefore, 32 Duroc × Landrace × Yorkshire 0-day-old newborn boars with similar body weights were randomly divided into 4 groups, with 8 replicates in each group and 1 pig in each replicate. All the piglets were breastfed. In addition, the piglets were given normal saline (CON group) or ferrous sulfate (OAFe group) or VA (VA group) or ferrous sulfate and VA (OAFe + VA group) on the 2 nd, 7 th, 12 th and 17 th day, respectively. The piglets were then slaughtered on the 21 st day, and intestinal samples were collected. The results showed that: (1) iron (P < 0.001) significantly increased the length, weight, relative weight, and the length to weight ratio of the small intestine. On the other hand, VA had a significant effect on the weight to length ratio (P = 0.015) and relative weight (P < 0.001) of the small intestine; (2) with regard to intestinal morphology, supplementation with iron (P <0.05) had obvious effects on the villus height (VH), crypt depth (CD), villus width (VW), and surface area. Additionally, both VA and interaction of VA and iron increased the VH (P < 0.05) and surface area (P = 0.001). The results also showed that iron (P < 0.01) increased the number of crypt goblet cells, Ki67 positive cells, and endocrine cells. Moreover, both VA and the interaction between VA and iron increased the number of endocrine cells in the villi (P = 0.05); (3) With regard to the mRNA expression levels of stem cell differentiation marker genes, iron (P < 0.05) decreased the expression of trophinin 2 (Trop2), leucine rich repeat containing G protein-coupled receptor 5 positive (Lgr5+), male specific lethal 1(Msl1), BMI 1 proto-oncogene, polycomb ring finger (Bmi1), achaete-scute family bHLH transcription factor 2 (Ascl2). On the other hand, VA increased the expression of Ascl2 (P = 0.001) although the interaction of VA and iron (P < 0.05) had an effect on the expression of secreted phosphoprotein 1 (Spp1) and Bmi1. In addition, VA decreased the gene or mRNA expression of aconitase 1 (Aco1; P < 0.001), transferrin receptor (TFRC; P = 0.001), and solute carrier family 11 member 2 (DMT1; P = 0.003) in the Iron Reactive Element/Iron Regulatory Protein (IRE/IRP) signaling pathway although iron and the interaction of VA and iron had no effect on the genes’ expression. The results therefore showed that VA, iron, and their interaction can promote intestinal development and epithelial cell differentiation in piglets.


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