Detection of C-MYC oncogene translocation and copy number change in the normal-dysplasia-carcinoma sequence of the larynx by fluorescence in situ hybridization

Context.— Fluorescence in situ hybridization (FISH) and brightfield in situ hybridization (ISH) are 2 clinically approved laboratory methods for detecting ERBB2 (HER2) amplification in breast cancer. Objective.— To compare the performance of FISH and brightfield ISH on proficiency testing administered by the College of American Pathologists Laboratory Accreditation Program. Design.— Retrospective review was performed on 70 tissue core samples in 7 separate proficiency testing surveys conducted between 2009 and 2013. Results.— The samples included 13 consensus-amplified tissue cores, 53 consensus-nonamplified cores, and 4 cores that did not reach consensus for FISH and/or brightfield ISH. There were 2552 individual responses for FISH and 1871 individual responses for brightfield ISH. Consensus response rates were comparable for FISH (2474 of 2524; 98.0%) and brightfield ISH (2135 of 2189; 97.5%). The FISH analysis yielded an average HER2 copy number per cell that was significantly higher (by 2.86; P = .02) compared with brightfield ISH for amplified cores. For nonamplified cores, FISH yielded slightly, but not significantly, higher (by 0.17; P = .10) HER2 copy numbers per cell. There was no significant difference in the average HER2 to control ratio for either consensus-amplified or consensus-nonamplified cores. Participants reported “unable to analyze” more frequently for brightfield ISH (244 of 2453; 9.9%) than they did for FISH (160 of 2684; 6.0%). Conclusions.— Our study indicates a high concordance rate in proficiency testing surveys, with some significant differences noted in the technical performance of these assays. In borderline cases, updated American Society of Clinical Oncology/College of American Pathologists cutoff thresholds that place greater emphasis on HER2 copy number per cell could accentuate those differences between FISH and brightfield ISH.

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TERT and AURKA Gene Copy Number Gains Enhance the Detection of Acral Lentiginous Melanomas by Fluorescence in Situ Hybridization

Journal of Molecular Diagnostics ◽

10.1016/j.jmoldx.2013.10.009 ◽

2014 ◽

Vol 16 (2) ◽

pp. 198-206 ◽

Cited By ~ 19

Author(s):

Alba Diaz ◽

Joan Anton Puig-Butillé ◽

Alexandra Valera ◽

Concha Muñoz ◽

Dolors Costa ◽

...

Keyword(s):

In Situ Hybridization ◽

Fluorescence In Situ Hybridization ◽

Copy Number ◽

Gene Copy Number ◽

Gene Copy

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Changes in the Cyclin D1 Gene Copy Number in Melanoma Cell Lines Detected by Double-Target Fluorescence In Situ Hybridization.

ACTA HISTOCHEMICA ET CYTOCHEMICA ◽

10.1267/ahc.32.431 ◽

1999 ◽

Vol 32 (5) ◽

pp. 431-436 ◽

Cited By ~ 1

Author(s):

Mayumi Matsuta ◽

Morimasa Matsuta ◽

Toshihide Akasaka ◽

Teruo Kagabu

Keyword(s):

In Situ Hybridization ◽

Fluorescence In Situ Hybridization ◽

Cyclin D1 ◽

Cell Lines ◽

Melanoma Cell ◽

Copy Number ◽

Gene Copy Number ◽

Gene Copy ◽

Melanoma Cell Lines

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A subset of gestational trophoblastic disease characterized by abnormal chromosome 8 copy number detected by fluorescence in situ hybridization

Cancer Genetics and Cytogenetics ◽

10.1016/s0165-4608(96)00439-6 ◽

1997 ◽

Vol 99 (1) ◽

pp. 24-29 ◽

Cited By ~ 6

Author(s):

H.F.L. Mark ◽

Alaa Afify ◽

William Taylor ◽

Kathleen Santoro ◽

John C. Lathrop

Keyword(s):

In Situ Hybridization ◽

Fluorescence In Situ Hybridization ◽

Copy Number ◽

Gestational Trophoblastic Disease ◽

Chromosome 8 ◽

Abnormal Chromosome ◽

Trophoblastic Disease

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Study of Chromosome 12 Copy Number in Breast Cancer Using Fluorescence In Situ Hybridization

Cancer Genetics and Cytogenetics ◽

10.1016/s0165-4608(98)00104-6 ◽

1999 ◽

Vol 108 (1) ◽

pp. 26-31 ◽

Cited By ~ 7

Author(s):

Hon Fong L Mark ◽

Stephen Brown ◽

William Taylor ◽

Nader Bassily ◽

Ci-Lin Sun ◽

...

Keyword(s):

Breast Cancer ◽

In Situ Hybridization ◽

Fluorescence In Situ Hybridization ◽

Copy Number ◽

Chromosome 12

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Increased chromosome 20 copy number detected by fluorescence in situ hybridization (FISH) in malignant melanoma

Genes Chromosomes and Cancer ◽

10.1002/(sici)1098-2264(199708)19:4<278::aid-gcc11>3.0.co;2-c ◽

1997 ◽

Vol 19 (4) ◽

pp. 278-285 ◽

Cited By ~ 21

Author(s):

James H. Barks ◽

Floyd H. Thompson ◽

Raymond Taetle ◽

Jin-Ming Yang ◽

John F. Stone ◽

...

Keyword(s):

In Situ Hybridization ◽

Malignant Melanoma ◽

Fluorescence In Situ Hybridization ◽

Copy Number ◽

Chromosome 20

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Chromosome Nomenclature and Cytological Characterization of Sacred Lotus

Cytogenetic and Genome Research ◽

10.1159/000486777 ◽

2017 ◽

Vol 153 (4) ◽

pp. 223-231 ◽

Cited By ~ 3

Author(s):

Zhuang Meng ◽

Xiaoxu Hu ◽

Zhiliang Zhang ◽

Zhanjie Li ◽

Qingfang Lin ◽

...

Keyword(s):

In Situ Hybridization ◽

Fluorescence In Situ Hybridization ◽

Genome Assembly ◽

Copy Number ◽

45S Rdna ◽

Chromosomal Structure ◽

Sacred Lotus ◽

Chromosomal Length

Sacred lotus is a basal eudicot plant that has been cultivated in Asia for over 7,000 years for its agricultural, ornamental, religious, and medicinal importance. A notable characteristic of lotus is the seed longevity. Extensive endeavors have been devoted to dissect its genome assembly, including the variety China Antique, which germinated from a 1,300-year-old seed. Here, cytogenetic markers representing the 10 largest megascaffolds, which constitute approximately 70% of the lotus genome assembly, were developed. These 10 megascaffolds were then anchored to the corresponding lotus chromosomes by fluorescence in situ hybridization using these cytogenetic markers, and a set of chromosome-specific cytogenetic markers that could unambiguously identify each of the 8 chromosomes was generated. Karyotyping was conducted, and a nomenclature based on chromosomal length was established for the 8 chromosomes of China Antique. Comparative karyotyping revealed relatively conserved chromosomal structures between China Antique and 3 modern cultivars. Interestingly, significant variations in the copy number of 45S rDNA were detected between China Antique and modern cultivars. Our results provide a comprehensive view on the chromosomal structure of sacred lotus and will facilitate further studies and the genome assembly of lotus.

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