Telecytology can be defined as the process of diagnostic cytology performed on digital images. The process of diagnostic cytology performed on digital images can be used for teaching, professional examinations, obtaining expert opinions on severe cases, and routine diagnosis of the entire laboratory workload. Ηowever, little information exists about its possible use for proficiency testing purposes. The existing studies have found a high (90%–95%) concordance between telecytological and glass slide diagnoses, but they refer to a small number of cases. Thus, the purpose of the chapter is to examine the feasibility of developing a proficiency testing scheme for cytology labs wishing to be accredited according to ISO 15189:2012 by using low-cost telemedical applications.