Hybrid inviability and differential submergence tolerance drive habitat segregation between two congeneric monkeyflowers

Ecology ◽  
2018 ◽  
Vol 99 (12) ◽  
pp. 2776-2786 ◽  
Author(s):  
Katherine Toll ◽  
John H. Willis

Rice Science ◽  
2021 ◽  
Vol 28 (1) ◽  
pp. 89-98
Author(s):  
Luo Yanchang ◽  
Ma Tingchen ◽  
Joanne Teo ◽  
Luo Zhixiang ◽  
Li Zefu ◽  
...  


2021 ◽  
Vol 22 (13) ◽  
pp. 6989
Author(s):  
Yuya Liang ◽  
Sudip Biswas ◽  
Backki Kim ◽  
Julia Bailey-Serres ◽  
Endang M. Septiningsih

Gene editing by use of clustered regularly interspaced short palindromic repeats (CRISPR) has become a powerful tool for crop improvement. However, a common bottleneck in the application of this approach to grain crops, including rice (Oryza sativa), is efficient vector delivery and calli regeneration, which can be hampered by genotype-dependent requirements for plant regeneration. Here, methods for Agrobacterium-mediated and biolistic transformation and regeneration of indica rice were optimized using CRISPR-Cas9 gene-editing of the submergence tolerance regulator SUBMERGENCE 1A-1 gene of the cultivar Ciherang-Sub1. Callus induction and plantlet regeneration methods were optimized for embryogenic calli derived from immature embryos and mature seed-derived calli. Optimized regeneration (95%) and maximal editing efficiency (100%) were obtained from the immature embryo-derived calli. Phenotyping of T1 seeds derived from the edited T0 plants under submergence stress demonstrated inferior phenotype compared to their controls, which phenotypically validates the disruption of SUB1A-1 function. The methods pave the way for rapid CRISPR-Cas9 gene editing of recalcitrant indica rice cultivars.





2010 ◽  
Vol 106 (6) ◽  
pp. 967-977 ◽  
Author(s):  
Michaela Sonnleitner ◽  
Ruth Flatscher ◽  
Pedro Escobar García ◽  
Jana Rauchová ◽  
Jan Suda ◽  
...  


2005 ◽  
Vol 148 (5) ◽  
pp. 1031-1038 ◽  
Author(s):  
G. I. Zardi ◽  
K. R. Nicastro ◽  
F. Porri ◽  
C. D. McQuaid


Evolution ◽  
2005 ◽  
Vol 59 (8) ◽  
pp. 1754-1767 ◽  
Author(s):  
Daniel I. Bolnick ◽  
Thomas J. Near


Sign in / Sign up

Export Citation Format

Share Document