Actin-binding proteins in mouse C2 myoblasts and myotubes: A combination of affinity chromatography and two-dimensional gel electrophoresis

1998 ◽  
Vol 19 (5) ◽  
pp. 826-833 ◽  
Author(s):  
Joëlle V. F. Coumans ◽  
Cristobal G. dos Remedios
Keyword(s):  
Affinity Chromatography ◽  
Gel Electrophoresis ◽  
Binding Proteins ◽  
Actin Binding ◽  
Two Dimensional ◽  
Actin Binding Proteins ◽  
Two Dimensional Gel Electrophoresis

scholarly journals Identification of Proteins Related to Nickel Homeostasis in Helicobater pylori by Immobilized Metal Affinity Chromatography and Two-Dimensional Gel Electrophoresis

2008 ◽  
Vol 2008 ◽  
pp. 1-6 ◽  
Author(s):  
Xuesong Sun ◽  
Ruiguang Ge ◽  
Jen-Fu Chiu ◽  
Hongzhe Sun ◽  
Qing-Yu He
Keyword(s):  
Affinity Chromatography ◽  
Gel Electrophoresis ◽  
Interacting Proteins ◽  
Peptic Ulcers ◽  
Two Dimensional ◽  
Two Dimensional Gel Electrophoresis ◽  
Iron Storage ◽  
Cell Extracts ◽  
Nickel Homeostasis ◽  
H Pylori

Helicobacter pylori (H. pylori) is a widespread human pathogen causing peptic ulcers and chronic gastritis. Maintaining nickel homeostasis is crucial for the establishment of H. pylori infection in humans. We used immobilized-nickel affinity chromatography to isolate Ni-related proteins from H. pylori cell extracts. Two-dimensional gel electrophoresis and mass spectrometry were employed to separate and identify twenty two Ni-interacting proteins in H. pylori. These Ni-interacting proteins can be classified into several general functional categories, including cellular processes (HspA, HspB, TsaA, and NapA), enzymes (Urease, Fumarase, GuaB, Cad, PPase, and DmpI), membrane-associated proteins (OM jhp1427 and HpaA), iron storage protein (Pfr), and hypothetical proteins (HP0271, HP jhp0216, HP jhp0301, HP0721, HP0614, and HP jhp0118). The implication of these proteins in nickel homeostasis is discussed.

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