Etoposide and merbarone are clastogenic and aneugenic in the mouse bone marrow micronucleus test complemented by fluorescence in situ hybridization with the mouseminor satellite DNA probe

2003 ◽  
Vol 41 (2) ◽  
pp. 99-103 ◽  
Author(s):  
S.M. Attia ◽  
U. Kliesch ◽  
G. Schriever-Schwemmer ◽  
O.A. Badary ◽  
F.M. Hamada ◽  
...  
Keyword(s):  
Bone Marrow ◽  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Satellite Dna ◽  
Micronucleus Test ◽  
Dna Probe ◽  
Mouse Bone Marrow ◽  
Bone Marrow Micronucleus

Fast-in Situ hybridization and immunoenzymatic color pigment detection of mouse bone marrow micronucleus

1999 ◽  
Vol 74 (3) ◽  
pp. 111-115 ◽  
Author(s):  
Christopher J. Kolanko ◽  
Mark D. Pyle ◽  
Harry Loats ◽  
Joseph Parton ◽  
William F. Blakely ◽  
...  
Keyword(s):  
Bone Marrow ◽  
In Situ Hybridization ◽  
Mouse Bone Marrow ◽  
Color Pigment ◽  
Bone Marrow Micronucleus

Analysis by fluorescence in situ hybridization with a mouse gamma satellite DNA probe of isolated micronuclei induced in mice by two clastogens and two spindle poisons

Mutagenesis ◽  
1995 ◽  
Vol 10 (6) ◽  
pp. 513-516 ◽  
Author(s):  
Jorma Mäki-Paakkanen ◽  
Makoto Hayashi ◽  
Takayoshi Suzuki ◽  
Hideyuki Tanabe ◽  
Masamitsu Honma ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Satellite Dna ◽  
Dna Probe

Mouse bone marrow micronucleus test results do not predict the germ cell mutagenicity of N-hydroxymethylacrylamide in the mouse dominant lethal assay

2003 ◽  
Vol 41 (2) ◽  
pp. 111-120 ◽  
Author(s):  
Kristine L. Witt ◽  
Lori A. Hughes ◽  
Leo T. Burka ◽  
Alfred F. McFee ◽  
James M. Mathews ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Germ Cell ◽  
Micronucleus Test ◽  
Mouse Bone Marrow ◽  
Test Results ◽  
Dominant Lethal ◽  
Dominant Lethal Assay ◽  
Bone Marrow Micronucleus

Early Detection of Relapse by Hypermetaphase Fluorescence In Situ Hybridization After Allogeneic Bone Marrow Transplantation for Chronic Myeloid Leukemia

2000 ◽  
Vol 18 (9) ◽  
pp. 1831-1836 ◽  
Author(s):  
Chy-Myong Seong ◽  
Sergio Giralt ◽  
Hagop Kantarjian ◽  
Jingping Xu ◽  
Jolynn Swantkowski ◽  
...  
Keyword(s):  
Bone Marrow ◽  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Low Frequency ◽  
Allogeneic Bone Marrow ◽  
Myelogenous Leukemia ◽  
Mixed Chimerism ◽  
Allogeneic Bone ◽  
Time Points

PURPOSE: Standard G-band cytogenetic analysis (CG) provides information on approximately 25 metaphases for monitoring the presence of Philadelphia chromosome positive (Ph+) cells in chronic myelogenous leukemia (CML) patients, making the detection of a low frequency of Ph+ cells problematic. The purpose of this study was to improve the detection of a low frequency of Ph+ cells. PATIENTS AND METHODS: We combined fluorescence in situ hybridization (FISH) with long-term colcemid exposure, capturing several hundred metaphases in bone marrow cultures (hypermetaphase FISH [HMF]). Using probes that identify Ph+ cells, HMF was compared with CG analysis in the follow-up evaluations of 51 patients with CML at various time points after allogeneic bone marrow transplant (BMT). RESULTS: Thirty-five patients never showed the presence of Ph+ cells by either method. In four patients, high frequencies of Ph+ cells were detected by both methods. In the remaining 12 patients, Ph+ cells were detected by HMF at time points after BMT when they were not detected by CG. In seven of the 12 patients, low but statistically significant frequencies of Ph+ cells (0.37% to 5.20%) were detected 3 months or later after BMT, and when no intervention was initiated, all seven patients later relapsed. Based on those data, an eighth patient with mixed chimerism and a similar HMF-detected Ph+ frequency (1.8% at 27 months after BMT) was reinfused with donor lymphocytes and achieved remission with 0% Ph+ cells studied by HMF (up to 50 months after BMT). Ph+ cells detected by HMF but not by CG less than 3 months after BMT disappeared on later examination in two of four patients. After detection of Ph+ cells by HMF only, the median time to cytogenetic progression (detection of Ph+ cells by CG) was 101 days. CONCLUSION: The results demonstrate the ability of HMF to detect low but clinically relevant levels of leukemic cells not detected by CG in transplant patients. The data indicate that HMF can detect low levels of Ph+ cells before standard cytogenetics at a time that may be useful in monitoring disease status and planning clinical interventions.

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