Role of short telomeres in inducing preferential chromosomal aberrations in human ovarian surface epithelial cells: A combined telomere quantitative fluorescence in situ hybridization and whole-chromosome painting study

2003 ◽  
Vol 37 (1) ◽  
pp. 92-97 ◽  
Author(s):  
Wen Deng ◽  
Sai Wah Tsao ◽  
Xin-Yuan Guan ◽  
Joe N. Lucas ◽  
Annie L. M. Cheung
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Chromosomal Aberrations ◽  
Chromosome Painting ◽  
Ovarian Surface Epithelial ◽  
Quantitative Fluorescence ◽  
Whole Chromosome Painting ◽  
Painting Study

Whole Chromosome Painting and Multiplex Fluorescence In Situ Hybridization in Detecting Complex Chromosomal Aberrations in Myelodysplastic Syndromes.

Blood ◽  
2006 ◽  
Vol 108 (11) ◽  
pp. 4853-4853
Author(s):  
Jianyong Li ◽  
Bing Xiao ◽  
Lijuan Chen ◽  
Yu Zhu ◽  
Wei Xu ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Chromosomal Aberrations ◽  
Myelodysplastic Syndromes ◽  
Chromosome Painting ◽  
Banding Technique ◽  
Marker Chromosomes ◽  
Molecular Cytogenetic ◽  
Whole Chromosome Painting

Abstract Objective To explore the value of the technique of whole chromosome painting (WCP) and multiplex fluorescence in situ hybridization (M-FISH) in the detection of complex chromosomal aberrations (CCAs) of myelodysplastic syndromes (MDS). Methods M-FISH was used in seven MDS patients with CCAs detected by R-banding technique to refine CCAs, and to identify cryptic translocations and characterization of marker chromosomes. Dual-color WCP procedures were further performed in 7 cases to confirm some rearrangements detected by M-FISH. Results In all cases, M-FISH confirmed all results of R-banding.The composition and origin of 6 kinds of marker chromosomes, 9 kinds of chromosomes with additional material undetermined and 5 kinds of derivative chromosomes undefined by CC were defined after M-FISH analysis; 4 kinds of cryptic translocations overlooked by CC were found on derivative chromosomes and previously normal appearing chromosomes. In addition, M-FISH revealed some nonrandom aberrations: aberrations involving chromosome 17 (5/7) and -5/5q- (4/7) were the two most frequent aberrations. Fluorescence flaring is a main factor leading to misinterpretations. Some misclassified and missed chromosomal aberrations by M-FISH were corrected by WCP. Conclusions M-FISH is a powerful molecular cytogenetic tool in clarification of CCAs. Complementary WCP helped us identify misclassified and missed chromosomal aberrations by M-FISH. CC in combination with molecular cytogenetic techniques M-FISH and WCP can unravel complex chromosomal aberrations more precisely.

scholarly journals Identification of satellited markers by microdissection and fluorescence in situ hybridization: a clinical case of isodicentric chromosome 22

2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Natalya A. Lemskaya ◽  
Svetlana A. Romanenko ◽  
Yulia V. Maksimova ◽  
Asia R. Shorina ◽  
Dmitry V. Yudkin
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Chromosome Painting ◽  
Chromosome Rearrangement ◽  
Marker Chromosome ◽  
Abnormal Development ◽  
Fish Analysis ◽  
Painting Probes ◽  
Whole Chromosome Painting

Abstract Background The presence of small supernumerary marker chromosomes (sSMCs) in a karyotype leads to diagnostic questions because the resulting extra material may cause abnormal development depending on the origin of the duplication/triplication. Because SMCs are so small, their origin cannot be determined by conventional cytogenetic techniques, and new molecular cytogenetic methods are necessary. Here, we applied a target approach to chromosome rearrangement analysis by isolating a chromosome of interest via microdissection and using it in fluorescence in situ hybridization (FISH) as a probe in combination with whole-chromosome painting probes. This approach allows to identify origins of both the euchromatin and repeat-rich regions of a marker. Case presentation We report a case of an adult male with congenital atresia of the rectum and anus, anotia, and atresia of the external auditory canal along with hearing loss. Karyotyping and FISH analysis with whole-chromosome painting probes of acrocentric chromosomes and the constructed microdissection library of the marker chromosome reliably identified an additional chromosome in some metaphases: mos 47,XY,+idic(22)(q11.2)[14]/46,XY [23]. Conclusion We propose to use whole-chromosome libraries and microdissected chromosomes in FISH to identify SMCs enriched with repeated sequences. We show that the methodology is successful in identifying the composition of a satellited marker chromosome.

scholarly journals Chromosomal bar codes produced by multicolor fluorescence in situ hybridization with multiple YAC clones and whole chromosome painting probes

1993 ◽  
Vol 2 (5) ◽  
pp. 505-512 ◽  
Author(s):  
Christoph Lengauer ◽  
Michael Speicher ◽  
Susanne Popp ◽  
Anna Jauch ◽  
Masafumi Taniwaki ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Chromosome Painting ◽  
Bar Codes ◽  
Multicolor Fluorescence ◽  
Painting Probes ◽  
Whole Chromosome Painting

Interphase Quantitative Fluorescence in Situ Hybridization (IQ-FISH)

2019 ◽  
Vol 3 (1) ◽  
Author(s):  
Ivan Y. Iourov ◽  
◽  
Ilia V. Soloviev ◽  
Yuri B. Yurov ◽  
Svetlana G. Vorsanova ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Quantitative Fluorescence

Chromosomal aberrations in atypical and anaplastic meningiomas: A fluorescence in situ hybridization study

2015 ◽  
Vol 63 (4) ◽  
pp. 517 ◽  
Author(s):  
Dwarakanath Srinivas ◽  
Vani Santosh ◽  
Sampath Somanna ◽  
Nishanth Sadashiva ◽  
Harsh Sugur
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Chromosomal Aberrations ◽  
Hybridization Study

Rapid-Fluorescence-in-Situ-Hybridization (FISH) on Uncultured Amniocytes for Avoiding Birth Defects due to Common Chromosomal Aberrations

2010 ◽  
Vol 7 (3) ◽  
pp. 163-168
Author(s):  
U Kandpal ◽  
M Mishra ◽  
A Fauzdar ◽  
M Chowdhry ◽  
RN Makroo ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Birth Defects ◽  
Chromosomal Aberrations

scholarly journals Fluorescence In Situ Hybridization (FISH) on Human Chromosomes Using Photoprobe Biotin-labeled Probes

2003 ◽  
Vol 51 (4) ◽  
pp. 549-551 ◽  
Author(s):  
Anja Weise ◽  
Peter Harbarth ◽  
Uwe Claussen ◽  
Thomas Liehr
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Yeast Artificial Chromosome ◽  
Artificial Chromosome ◽  
Dna Probes ◽  
Human Chromosomes ◽  
First Time ◽  
Established Technique ◽  
Whole Chromosome Painting

Fluorescence in situ hybridization (FISH) on human chromosomes in meta-and interphase is a well-established technique in clinical and tumor cytogenetics and for studies of evolution and interphase architecture. Many different protocols for labeling the DNA probes used for FISH have been published. Here we describe for the first time the successful use of Photoprobe biotin-labeled DNA probes in FISH experiments. Yeast artificial chromosome (YAC) and whole chromosome painting (wcp) probes were tested.

Detection of numerical chromosomal aberrations in malignant melanomas using fluorescence in situ hybridization

1997 ◽  
Vol 24 (4) ◽  
pp. 201-205 ◽  
Author(s):  
Mayumi Matsuta ◽  
Yuko Imamura ◽  
Morimasa Matsuta ◽  
Kohsuke Sasaki ◽  
Saiichi Kon
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Chromosomal Aberrations ◽  
Malignant Melanomas

scholarly journals Chromosomal aberrations detected by Fluorescence in situ hybridization in 344 Brazilian chronic lymphocytic leukemia patients

2017 ◽  
Vol 39 (4) ◽  
pp. 388-390
Author(s):  
Maria Eduarda Sanseverino de Lourenço da Motta Zorovich ◽  
Denise Albuquerque Dourado ◽  
Maria de Lourdes Lopes Ferrari Chauffaille
Keyword(s):  
In Situ Hybridization ◽  
Chronic Lymphocytic Leukemia ◽  
Fluorescence In Situ Hybridization ◽  
Chromosomal Aberrations ◽  
Lymphocytic Leukemia
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