scholarly journals Hepatitis C Virus Mediated Inhibition of miR‐181c Activates ATM Signaling and Promotes Hepatocyte Growth

Hepatology ◽  
2019 ◽  
Vol 71 (3) ◽  
pp. 780-793 ◽  
Author(s):  
Tapas Patra ◽  
Keith Meyer ◽  
Ratna B. Ray ◽  
Ranjit Ray
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Atm Signaling ◽  
Hepatocyte Growth

scholarly journals Hepatitis C Virus Core Protein and Cellular Protein HAX-1 Promote 5-Fluorouracil-Mediated Hepatocyte Growth Inhibition

2009 ◽  
Vol 83 (19) ◽  
pp. 9663-9671 ◽  
Author(s):  
Arup Banerjee ◽  
Kousuke Saito ◽  
Keith Meyer ◽  
Sutapa Banerjee ◽  
Malika Ait-Goughoulte ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Growth Inhibition ◽  
Hepg2 Cells ◽  
Core Protein ◽  
Cellular Protein ◽  
P53 Expression ◽  
Hcv Core Protein ◽  
Caspase 7 ◽  
Hepatocyte Growth

ABSTRACT Hepatitis C virus (HCV) often causes chronic infection and may lead to hepatocellular carcinoma (HCC). We have shown previously that HCV core protein has pleiotropic functions, including transcriptional regulation of a number of cellular genes, although the mechanism for gene regulation remains unclear. In this study, a mammalian two-hybrid screen identified a novel binding partner, HS1-associated protein X-1 (HAX-1), for HCV core protein from a human liver cDNA library. An association between HAX-1 and HCV core protein was further verified by confocal microscopy and coimmunoprecipitation in HepG2 cells expressing HCV core or full-length (FL) gene. Both HCV core protein and a chemotherapeutic agent for HCC, 5-flouorouracil (5-FU), are known to modulate p53. We examined here whether an association between core and HAX-1 has any functional relevance to p53 modulation in 5-FU-treated cells. For this, the role of HAX-1 on 5-FU treatment was examined in HepG2 cells expressing HCV core or FL gene using cell proliferation, p53 expression, and caspase activation analysis. Cells expressing HCV-core or FL gene were more susceptible to 5-FU-induced growth inhibition than control cells, whereas cell survival was enhanced after suppression of HAX-1 by small interfering RNA. Further, 5-FU-mediated p53 expression was reduced with concurrent HAX-1 suppression in core- or polyprotein-expressing cells compared to control HepG2 cells, and caspase-2 and -7 activities were diminished. On the other hand, HCV core protein did not play a detectable role in 5-FU-mediated caspase-7 activation in the absence of functional p53 in Hep3B or Huh-7 cells. These observations underscore an association between HCV core and HAX-1, which promotes 5-FU mediated p53-dependent caspase-7 activation and hepatocyte growth inhibition.

Abstract 3177: Hepatitis C virus transcriptionally downregulates miR-181c for promotion of hepatocyte growth towards hepatocellular carcinoma

2014 ◽  
Author(s):  
Ratna B. Ray ◽  
Anupam Mukherjee ◽  
Shubham Shrivastava ◽  
Ranjit Ray
Keyword(s):  
Hepatocellular Carcinoma ◽  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Hepatocyte Growth

Increased miR-16 expression induced by hepatitis C virus infection promotes liver fibrosis through downregulation of hepatocyte growth factor and Smad7

2015 ◽  
Vol 160 (8) ◽  
pp. 2043-2050 ◽  
Author(s):  
Bin Zhu ◽  
Xiao-xia Wei ◽  
Tian-bao Wang ◽  
Yan-cai Zhou ◽  
A-min Liu ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Growth Factor ◽  
Liver Fibrosis ◽  
Virus Infection ◽  
Hepatocyte Growth Factor ◽  
Hepatitis C Virus Infection ◽  
Hepatocyte Growth

scholarly journals Association between MicroRNA-373 and Long Noncoding RNA NORAD in Hepatitis C Virus-Infected Hepatocytes Impairs Wee1 Expression for Growth Promotion

2018 ◽  
Vol 92 (20) ◽  
Author(s):  
Subhayan Sur ◽  
Reina Sasaki ◽  
Pradip Devhare ◽  
Robert Steele ◽  
Ranjit Ray ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Long Noncoding Rna ◽  
Noncoding Rna ◽  
Growth Regulation ◽  
Growth Promotion ◽  
Hcv Infection ◽  
Direct Target ◽  
Future Therapy ◽  
Hepatocyte Growth

ABSTRACT Chronic hepatitis C virus (HCV) infection may lead to end-stage liver disease, including hepatocellular carcinoma (HCC). We have shown previously that microRNA-373 (miR-373) is upregulated in HCV-infected human liver biopsy specimens. To gain insight into the role of miR-373 in HCV-mediated pathogenesis, we investigated its interacting partner for hepatocyte growth regulation. Transcriptome sequencing (RNA-seq) data revealed that Wee1 is associated with miR-373 and is a direct target. Interestingly, higher expression of Wee1 was noted in HCV-infected hepatocytes than in uninfected hepatocytes, suggesting that other factors may block miR-373-mediated Wee1 inhibition. We subsequently found an association between the long noncoding RNA NORAD (LINC00657) and miR-373, and we demonstrated that NORAD binds to miR-373 and Wee1 independently. However, the high level of Wee1 expression in HCV-infected hepatocytes suggested that miR-373 forms a complex with NORAD. Depletion of miR-373 or the inhibitor Wee1 reduces the growth of Huh7.5 cells harboring the HCV genome as well as reducing Wee1 expression. Taken together, our data demonstrate a novel mechanism of hepatocyte growth promotion during HCV infection involving a miR-373–NORAD–Wee1 axis, which may be a target for future therapy against HCV-associated HCC. IMPORTANCE The mechanism of HCV-mediated liver pathogenesis is poorly understood. In this study, we observed that HCV infection upregulates miR-373 and Wee1, a pivotal player in the G2 checkpoint in the cell cycle, although Wee1 is a direct target for miR-373. Subsequent investigation demonstrated that miR-373 forms a complex with the long noncoding RNA NORAD, resulting in the release of their common target, Wee1, in HCV-infected cells, which, in turn, favors uncontrolled cell growth. Our study suggested a previously unknown mechanism for hepatocyte growth promotion following HCV infection, and this pathway can be targeted for future therapy against HCV-mediated liver pathogenesis.

Hepatocyte growth factor protects the liver against hepatitis C virus in patients on regular hemodialysis

1998 ◽  
Vol 10 (2) ◽  
pp. 164-166 ◽  
Author(s):  
T. Rampino ◽  
C. Libetta ◽  
A. Mazzone ◽  
M. Gregorini ◽  
G. Soccio ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Growth Factor ◽  
Hepatocyte Growth Factor ◽  
Hepatocyte Growth ◽  
Regular Hemodialysis

435 Serum concentration of hepatocyte growth factor is related to histological fibrosis stage in patients with hepatitis C virus chronic liver disease

2006 ◽  
Vol 44 ◽  
pp. S163
Author(s):  
E. Marín-Serrano ◽  
J.A. Girón-González ◽  
M. Tejada-Cabrera ◽  
C. Rodríguez-Ramos ◽  
E. Díaz-García ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Liver Disease ◽  
Hepatitis C ◽  
Growth Factor ◽  
Serum Concentration ◽  
Hepatocyte Growth Factor ◽  
Chronic Liver Disease ◽  
Chronic Liver ◽  
Fibrosis Stage ◽  
Hepatocyte Growth

Effect of hemodialysis schedules and membranes on hepatocyte growth factor and hepatitis C virus RNA levels

2010 ◽  
Vol 82 (5) ◽  
pp. 763-767 ◽  
Author(s):  
Guillermina Barril ◽  
Javier Bartolomé ◽  
Paloma Sanz ◽  
Emmanuela Buoncristiani ◽  
Juan Antonio Traver ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Growth Factor ◽  
Hepatocyte Growth Factor ◽  
Hepatitis C Virus Rna ◽  
Virus Rna ◽  
Hepatocyte Growth ◽  
Rna Levels

scholarly journals Hemodialysis prevents liver disease caused by hepatitis C virus: Role of hepatocyte growth factor

1999 ◽  
Vol 56 (6) ◽  
pp. 2286-2291 ◽  
Author(s):  
Teresa Rampino ◽  
Eloisa Arbustini ◽  
Marilena Gregorini ◽  
Paola Guallini ◽  
Carmelo Libetta ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Liver Disease ◽  
Hepatitis C ◽  
Growth Factor ◽  
Hepatocyte Growth Factor ◽  
Hepatocyte Growth

Hepatitis C virus core protein stimulates hepatocyte growth: Correlation with upregulation of wnt-1 expression

Hepatology ◽  
2005 ◽  
Vol 41 (5) ◽  
pp. 1096-1105 ◽  
Author(s):  
Takayoshi Fukutomi ◽  
Yonghong Zhou ◽  
Shigenobu Kawai ◽  
Hidetoshi Eguchi ◽  
Jack R. Wands ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Core Protein ◽  
Virus Core ◽  
Hepatocyte Growth
Sign in / Sign up

Export Citation Format

Share Document