Increase in viral antigen in individual polyoma-virus-infected mouse kidney cells as studied by quantitative immunofluorescence

1973 ◽  
Vol 11 (3) ◽  
pp. 694-703
Author(s):  
A. Levin ◽  
D. Killander ◽  
B. Nordenskjöld
Keyword(s):  
Infected Mouse ◽  
Viral Antigen ◽  
Mouse Kidney ◽  
Polyoma Virus ◽  
Kidney Cells ◽  
Quantitative Immunofluorescence

DNA polymerases in polyoma virus-infected mouse kidney cells.

1975 ◽  
Vol 16 (5) ◽  
pp. 1095-1100 ◽  
Author(s):  
U Wintersberger ◽  
E Wintersberger
Keyword(s):  
Infected Mouse ◽  
Dna Polymerases ◽  
Mouse Kidney ◽  
Polyoma Virus ◽  
Kidney Cells

Fibronectin gene expression in proliferating, quiescent, and SV40-infected mouse kidney cells

1992 ◽  
Vol 202 (2) ◽  
pp. 464-470 ◽  
Author(s):  
Edward W. Khandjian ◽  
Consuelo Salomon ◽  
Nicole Léonard ◽  
Sandra Tremblay ◽  
Hans Türler
Keyword(s):  
Gene Expression ◽  
Infected Mouse ◽  
Mouse Kidney ◽  
Kidney Cells

Synthesis of polyoma virus structural polypeptides in mouse kidney cells

Virology ◽  
1974 ◽  
Vol 58 (1) ◽  
pp. 75-85 ◽  
Author(s):  
J.G. Seehafer ◽  
R. Weil
Keyword(s):  
Mouse Kidney ◽  
Polyoma Virus ◽  
Kidney Cells

The recovery of polyoma virus from infected mouse cells: relevance to virus purification

1971 ◽  
Vol 17 (6) ◽  
pp. 775-781 ◽  
Author(s):  
Larry M. Kohse ◽  
Lorraine McGrath ◽  
James B. Hudson
Keyword(s):  
Tissue Culture ◽  
Infected Mouse ◽  
Mouse Embryo ◽  
Mouse Kidney ◽  
Polyoma Virus ◽  
Mouse Tissue ◽  
Virus Purification ◽  
Viral Capsids ◽  
Mouse Cells ◽  
Cell Fragments

The desire to obtain maximum yields of polyoma virus from infected mouse tissue culture lysates prompted us to examine more closely the interaction between virus and cell fragments. The objective was to find the best and most economical means of alleviating this interaction, and allow quantitative recovery of the virus for further purification. Sonication of polyoma virus infected mouse-kidney cultures sufficed to release the virus in a form amenable to hemagglutinin assay. In contrast infected mouse embryo lysates required neuraminidase to effect maximum release. A variety of other enzymes and treatments were tested and found to be completely ineffective, or less effective than neuraminidase. However, in spite of the ability to effect release of virus at this stage, subsequent centrifugation to pellet the cell fragments always resulted in reattachment of the virus to these fragments. Complete elution of the virus then required several cycles of neuraminidase, trypsin, pH 8.5 treatments. It was shown that full and empty viral capsids behaved similarly.

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