Rab3A Inhibition of Ca 2+ ‐Dependent Dopamine Release From PC12 Cells Involves Interaction With Synaptotagmin I

2017 ◽  
Vol 118 (11) ◽  
pp. 3696-3705 ◽  
Author(s):  
Zhipan Dai ◽  
Xia Tang ◽  
Jia Chen ◽  
Xiaochao Tang ◽  
Xianchun Wang
Keyword(s):  
Pc12 Cells ◽  
Dopamine Release ◽  
Synaptotagmin I

scholarly journals The C2b Domain of Synaptotagmin Is a Ca2+–Sensing Module Essential for Exocytosis

2000 ◽  
Vol 150 (5) ◽  
pp. 1125-1136 ◽  
Author(s):  
Radhika C. Desai ◽  
Bimal Vyas ◽  
Cynthia A. Earles ◽  
J. Troy Littleton ◽  
Judith A. Kowalchyck ◽  
...  
Keyword(s):  
Synaptic Vesicle ◽  
Pc12 Cells ◽  
Conformational Changes ◽  
Cytoplasmic Domain ◽  
Dominant Negative ◽  
Fusion Pore ◽  
Vesicle Membrane ◽  
C2 Domains ◽  
Essential Step ◽  
Synaptotagmin I

The synaptic vesicle protein synaptotagmin I has been proposed to serve as a Ca2+ sensor for rapid exocytosis. Synaptotagmin spans the vesicle membrane once and possesses a large cytoplasmic domain that contains two C2 domains, C2A and C2B. Multiple Ca2+ ions bind to the membrane proximal C2A domain. However, it is not known whether the C2B domain also functions as a Ca2+-sensing module. Here, we report that Ca2+ drives conformational changes in the C2B domain of synaptotagmin and triggers the homo- and hetero-oligomerization of multiple isoforms of the protein. These effects of Ca2+ are mediated by a set of conserved acidic Ca2+ ligands within C2B; neutralization of these residues results in constitutive clustering activity. We addressed the function of oligomerization using a dominant negative approach. Two distinct reagents that block synaptotagmin clustering potently inhibited secretion from semi-intact PC12 cells. Together, these data indicate that the Ca2+-driven clustering of the C2B domain of synaptotagmin is an essential step in excitation-secretion coupling. We propose that clustering may regulate the opening or dilation of the exocytotic fusion pore.

Latroeggtoxin-VI and its effect on dopamine release from PC12 cells

Toxicon ◽  
2019 ◽  
Vol 158 ◽  
pp. S59-S60
Author(s):  
Xiaochao Tang ◽  
Shuai Yan ◽  
Dehong Xu ◽  
Xianchun Wang
Keyword(s):  
Pc12 Cells ◽  
Dopamine Release

scholarly journals Contribution of capacitative Ca2+-entry to dopamine release mediated by P2U-puri uoceptors in PC12 cells.

1996 ◽  
Vol 71 ◽  
pp. 60
Author(s):  
Schuichi Koizumi ◽  
Ken Nakazawa ◽  
Yasuo Ohno ◽  
Kazuhide Inoue
Keyword(s):  
Pc12 Cells ◽  
Dopamine Release

Effects of direct exposure of green odour components on dopamine release from rat brain striatal slices and PC12 cells

2008 ◽  
Vol 75 (5) ◽  
pp. 706-712 ◽  
Author(s):  
Hironari Kako ◽  
Syuichi Fukumoto ◽  
Yoko Kobayashi ◽  
Hidehiko Yokogoshi
Keyword(s):  
Rat Brain ◽  
Pc12 Cells ◽  
Dopamine Release ◽  
Striatal Slices ◽  
Direct Exposure

Oxidative metabolite of dopamine, 3,4-dihydroxyphenylacetaldehyde, induces dopamine release from PC12 cells by a Ca2+-independent mechanism

2002 ◽  
Vol 931 (1) ◽  
pp. 96-99 ◽  
Author(s):  
Tsuneichi Hashimoto ◽  
Chihiro Yabe-Nishimura
Keyword(s):  
Pc12 Cells ◽  
Dopamine Release ◽  
Independent Mechanism

Mechanism of 1-Methyl-4-Phenylpyridinium-Induced Dopamine Release from PC12 Cells

2005 ◽  
Vol 30 (5) ◽  
pp. 633-639 ◽  
Author(s):  
Jaturaporn Chagkutip ◽  
Piyarat Govitrapong ◽  
Sirirat Klongpanichpak ◽  
Manuchair Ebadi
Keyword(s):  
Pc12 Cells ◽  
Dopamine Release

Expression of synaptotagmin I or II promotes neurite outgrowth in PC12 cells

2000 ◽  
Vol 295 (1-2) ◽  
pp. 33-36 ◽  
Author(s):  
Mitsunori Fukuda ◽  
Katsuhiko Mikoshiba
Keyword(s):  
Neurite Outgrowth ◽  
Pc12 Cells ◽  
Synaptotagmin I

Depolarization-induced changes of free intracellular Ca2+ concentration and of [3H]dopamine release in undifferentiated and differentiated PC12 cells

1990 ◽  
Vol 17 (2) ◽  
pp. 197-203 ◽  
Author(s):  
B.F.X. Reber ◽  
H. Porzig ◽  
C. Becker ◽  
H. Reuter
Keyword(s):  
Pc12 Cells ◽  
Dopamine Release ◽  
Differentiated Pc12 Cells ◽  
Induced Changes

Effects of scoparone on dopamine release in PC12 cells

Fitoterapia ◽  
2010 ◽  
Vol 81 (6) ◽  
pp. 497-502 ◽  
Author(s):  
Yoo Jung Yang ◽  
Hak Ju Lee ◽  
Byung Koo Lee ◽  
Sung Cil Lim ◽  
Chong Kil Lee ◽  
...  
Keyword(s):  
Pc12 Cells ◽  
Dopamine Release
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