Effects of Hypoxia and Hypercapnic Hypoxia on Oxygen Transport and Acid-Base Status in the Atlantic Blue Crab, Callinectes sapidus
, During Exercise

In the days immediately after moulting, manipulations of external pH, [HCO3−], and [Ca2+] were used to determine the nature of the rapid net Ca2+ influx and attendant apparent net H+ efflux in the blue crab (Callinectes sapidus Rathbun). Both fluxes were strongly inhibited by reductions in external [Ca2+], [HCO3−], or pH. The net Ca2+ influx was reversed at an external concentration of 2.5 mmol l−1, and both fluxes were reversed by reducing the external [HCO3−] to 0.2 mmol l−1. The correlation between net Ca2+ flux and apparent net H+ flux was 0.61 (P<0.01), but the variability and the time course of most experiments indicated that the link was indirect, rather than a direct coupling or cotransport. This conclusion was also borne out by acid-base disturbances that occurred in the low-[Ca2+] treatment. The results are consistent with the hypothesis that inward calcium transport is accompanied by both inward HCO3− transport and outward H+ transport, probably by separate exchanges with ions of like charge such as Na+ and Cl−. Crustecdysone (β-ecdysone) does not appear to be involved in control of these post-moult fluxes and calcification.

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Blood oxygen transport and acid-base status of stressed trout (Salmo gairdnerii): Pre- and postbranchial values in winter fish

Comparative Biochemistry and Physiology Part A Physiology ◽

10.1016/0300-9629(86)90634-1 ◽

1986 ◽

Vol 84 (2) ◽

pp. 391-396 ◽

Cited By ~ 28

Author(s):

Mikko Nikinmaa ◽

Frank B. Jensen

Keyword(s):

Oxygen Transport ◽

Blood Oxygen ◽

Acid Base ◽

Acid Base Status

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Gene transcripts encoding hypoxia-inducible factor (HIF) exhibit tissue- and muscle fiber type-dependent responses to hypoxia and hypercapnic hypoxia in the Atlantic blue crab, Callinectes sapidus

Comparative Biochemistry and Physiology Part A Molecular & Integrative Physiology ◽

10.1016/j.cbpa.2012.05.195 ◽

2012 ◽

Vol 163 (1) ◽

pp. 137-146 ◽

Cited By ~ 24

Author(s):

Kristin M. Hardy ◽

Chandler R. Follett ◽

Louis E. Burnett ◽

Sean C. Lema

Keyword(s):

Blue Crab ◽

Muscle Fiber ◽

Callinectes Sapidus ◽

Fiber Type ◽

Hypoxia Inducible Factor ◽

Muscle Fiber Type ◽

Hypercapnic Hypoxia ◽

Gene Transcripts

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Haemolymph oxygen transport and acid-base status in Glyptonotus antarcticus Eights

Polar Biology ◽

10.1007/s003000050153 ◽

1997 ◽

Vol 18 (1) ◽

pp. 10-15 ◽

Cited By ~ 9

Author(s):

N. M. Whiteley ◽

E. W. Taylor ◽

A. Clarke ◽

A. J. El Haj

Keyword(s):

Oxygen Transport ◽

Acid Base ◽

Acid Base Status

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Hemolymph oxygen transport, acid-base status, and hydromineral regulation during dehydration in three terrestrial crabs,Cardisoma,Birgus, andCoenobita

Journal of Experimental Zoology ◽

10.1002/jez.1402180107 ◽

1981 ◽

Vol 218 (1) ◽

pp. 53-64 ◽

Cited By ~ 27

Author(s):

Warren W. Burggren ◽

Brian R. McMahon

Keyword(s):

Oxygen Transport ◽

Acid Base ◽

Acid Base Status ◽

Hydromineral Regulation

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Acid-Base Balance in Callinectes Sapidus During Acclimation from High to Low Salinity

Journal of Experimental Biology ◽

10.1242/jeb.101.1.255 ◽

1982 ◽

Vol 101 (1) ◽

pp. 255-264 ◽

Cited By ~ 7

Author(s):

RAYMOND P. HENRY ◽

JAMES N. CAMERON

Keyword(s):

Carbonic Anhydrase ◽

Blue Crab ◽

Callinectes Sapidus ◽

Weak Acid ◽

Strong Ion Difference ◽

Acid Base Balance ◽

Acid Base ◽

Ion Regulation ◽

Low Salinity ◽

Base Balance

When transferred from 865 to 250 m-osmol salinity, the blue crab C. sapidus maintains its blood Na+ and Cl− concentrations significantly above those in the medium. When branchial carbonic anhydrase is inhibited by acetazolamide, ion regulation fails and the animals do not survive the transfer. An alkalosis occurs in the blood at low salinity, indicated by an increase in HCO3− and pH at constant PCO2. The alkalosis is closely correlated with an increase in the Na+-Cl− difference, a convenient indicator of the overall strong ion difference. The contribution of changes in PCO2 to acid-base changes was negligible, but the change in the total weak acid (proteins) may be important. It is suggested that the change in blood acidbase status with salinity is related to an increase in the strong ion difference, which changes during the transition from osmoconformity to osmoregulation in the blue crab, and which is related to both carbonic anhydrase and ionactivated ATPases. Note:

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