Lectin-binding pattern as tool to identify and enrich specific primary testis cells of the tilapia (Oreochromis niloticus) and medaka (Oryzias latipes)

Author(s):  
Sergey V. Tokalov ◽  
Herwig O. Gutzeit
2013 ◽  
Vol 48 (5) ◽  
pp. 850-857 ◽  
Author(s):  
CG Barbeito ◽  
HH Ortega ◽  
V Matiller ◽  
EJ Gimeno ◽  
NR Salvetti

2012 ◽  
Vol 75 (8) ◽  
pp. 1124-1135 ◽  
Author(s):  
Gianluca Accogli ◽  
Sara Zizza ◽  
Angel García-lópez ◽  
Carmen Sarasquete ◽  
Salvatore Desantis

10.4081/846 ◽  
2009 ◽  
Vol 47 (4) ◽  
pp. 353 ◽  
Author(s):  
F Parillo ◽  
C Dall’Aglio ◽  
A Verini Supplizi ◽  
P Ceccarelli ◽  
AM Gargiulo

An ultrastructural localization of lectin receptors on the zona pellucida (ZP) of porcine antral oocytes and on the granulosa cells was performed using a panel of horseradish peroxidase- labelled lectins in conjunction with antiperoxidase antibody and protein A-gold. In some cases, lectin incubation was preceded by sialidase digestion. WGA-, Con-A-, UEA-I-, RCA-I-, PNA- and SBA-reactive sites were distributed differently in the porcine ZP. Sialidase digestion increased the positivity obtained with RCA-I and it was necessary to promote PNA and SBA reactivity. These results indicated that the ZP contained N-acetylglucosamine, a-mannose, a- fucose, b-Gal-(1-4)GlcNAc, b-Gal- (1-3)GalNAc, b-GalNAc and sialic acid residues. We also observed the presence of vesicles in both the ooplasm and granulosa cells, showing a similar lectin binding pattern to that of the ZP, thus suggesting that the oocyte and granulosa cells are the site of synthesis of ZP glucidic determinants.


2001 ◽  
Vol 11 (3) ◽  
pp. 205-212 ◽  
Author(s):  
A. Lityńska ◽  
M. Przybyuulo ◽  
E. Pocheć ◽  
D. Hoja-uuLukowicz ◽  
D. Ciouulczyk ◽  
...  

1986 ◽  
Vol 85 (6) ◽  
pp. 515-521 ◽  
Author(s):  
K. Jezernik ◽  
N. Pipan

2012 ◽  
Vol 74 (2) ◽  
pp. 155-160 ◽  
Author(s):  
Tetsuya SHIMOKAWA ◽  
Takuya DOIHARA ◽  
Manami MAKARA ◽  
Kyoji MIYAWAKI ◽  
Hiroaki NABEKA ◽  
...  

1995 ◽  
Vol 69 (3) ◽  
pp. 160-164 ◽  
Author(s):  
M. R. Carratù ◽  
M. Labate ◽  
S. De Santis ◽  
A. Giustino ◽  
M. A. De Salvia ◽  
...  

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