Lectin binding pattern of schwann cells and macrophages in 2,5-hexanedione-induced axonal degeneration in rats

Areas of demyelination can be remyelinated by transplanting myelin-forming cells. Schwann cells are the naturally remyelinating cells of the peripheral nervous system and have a number of features that may make them attractive for cell implantation therapies in multiple sclerosis, in which spontaneous but limited Schwann cell remyelination has been well documented. Schwann cells can be expanded in vitro, potentially affording the opportunity of autologous transplantation; and they might also be spared the demyelinating process in multiple sclerosis. Although rat, cat, and monkey Schwann cells have been transplanted into rodent demyelinating lesions, the behavior of transplanted human Schwann cells has not been evaluated. In this study we examined the consequences of injecting human Schwann cells into areas of acute demyelination in the spinal cords of adult rats. We found that transplants containing significant fibroblast contamination resulted in deposition of large amounts of collagen and extensive axonal degeneration. However, Schwann cell preparations that had been purified by positive immunoselection using antibodies to human low-affinity nerve growth factor receptor containing less than 10% fibroblasts were associated with remyelination. This result indicates that fibroblast contamination of human Schwann cells represents a greater problem than would have been appreciated from previous studies.

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Lectin-Binding Pattern in Ovarian Structures of Rats with Experimental Polycystic Ovaries

Reproduction in Domestic Animals ◽

10.1111/rda.12174 ◽

2013 ◽

Vol 48 (5) ◽

pp. 850-857 ◽

Cited By ~ 3

Author(s):

CG Barbeito ◽

HH Ortega ◽

V Matiller ◽

EJ Gimeno ◽

NR Salvetti

Keyword(s):

Lectin Binding ◽

Binding Pattern ◽

Polycystic Ovaries

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Lectin-binding pattern of the senegalese sole Solea senegalensis oogenesis

Microscopy Research and Technique ◽

10.1002/jemt.22040 ◽

2012 ◽

Vol 75 (8) ◽

pp. 1124-1135 ◽

Cited By ~ 4

Author(s):

Gianluca Accogli ◽

Sara Zizza ◽

Angel García-lópez ◽

Carmen Sarasquete ◽

Salvatore Desantis

Keyword(s):

Lectin Binding ◽

Solea Senegalensis ◽

Senegalese Sole ◽

Binding Pattern

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Immunogold study on lectin binding in the porcine zona pellucida and granulosa cells

European Journal of Histochemistry ◽

10.4081/846 ◽

2009 ◽

Vol 47 (4) ◽

pp. 353 ◽

Cited By ~ 9

Author(s):

F Parillo ◽

C Dallâ€™Aglio ◽

A Verini Supplizi ◽

P Ceccarelli ◽

AM Gargiulo

Keyword(s):

Sialic Acid ◽

Horseradish Peroxidase ◽

Granulosa Cells ◽

Zona Pellucida ◽

Lectin Binding ◽

Protein A ◽

Ultrastructural Localization ◽

Binding Pattern ◽

Con A ◽

Lectin Receptors

An ultrastructural localization of lectin receptors on the zona pellucida (ZP) of porcine antral oocytes and on the granulosa cells was performed using a panel of horseradish peroxidase- labelled lectins in conjunction with antiperoxidase antibody and protein A-gold. In some cases, lectin incubation was preceded by sialidase digestion. WGA-, Con-A-, UEA-I-, RCA-I-, PNA- and SBA-reactive sites were distributed differently in the porcine ZP. Sialidase digestion increased the positivity obtained with RCA-I and it was necessary to promote PNA and SBA reactivity. These results indicated that the ZP contained N-acetylglucosamine, a-mannose, a- fucose, b-Gal-(1-4)GlcNAc, b-Gal- (1-3)GalNAc, b-GalNAc and sialic acid residues. We also observed the presence of vesicles in both the ooplasm and granulosa cells, showing a similar lectin binding pattern to that of the ZP, thus suggesting that the oocyte and granulosa cells are the site of synthesis of ZP glucidic determinants.

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