Electrical and ionic controls of tissue cell locomotion in DC electric fields

1985 ◽  
Vol 13 (1-2) ◽  
pp. 223-244 ◽  
Author(s):  
M.S. Cooper ◽  
M. Schliwa
Keyword(s):  
Electric Fields ◽  
Tissue Cell ◽  
Cell Locomotion ◽  
Dc Electric Fields

scholarly journals Motility of cultured fish epidermal cells in the presence and absence of direct current electric fields.

1986 ◽  
Vol 102 (4) ◽  
pp. 1384-1399 ◽  
Author(s):  
M S Cooper ◽  
M Schliwa
Keyword(s):  
Membrane Potential ◽  
Electric Field ◽  
Electric Fields ◽  
Direct Current ◽  
Epidermal Cells ◽  
Resting Membrane Potential ◽  
Cell Clusters ◽  
Cell Locomotion ◽  
Presence And Absence ◽  
Dc Electric Fields

The motile behavior and cytoskeletal structures of fish epidermal cells (keratocytes) in the presence and absence of direct current (DC) electric fields were examined. These cells spontaneously show highly directional locomotion in culture, migrating at rates of up to 1 micron/s. When DC electric fields between 0.5 and 15 V/cm are applied, single epidermal cells as well as cell clusters and cell sheets migrate towards the cathode. Cell clusters and sheets break apart into single migratory cells in the upper range of these field strengths. Cell shape and morphology are unaltered when the keratocytes are guided by an electric field. Neither the spontaneous locomotion nor the electrically guided motility were found to be microtubule dependent. 1 mM La3+, 10 mM Co2+, 50 microM verapamil, and 50 microM nitrendipine (calcium channel antagonists) reversibly inhibited lamellipod formation and cell locomotion in both spontaneously migrating and electrically guided cells. Ciba-Geigy Product 28392, which stimulates the opening of calcium channels, and is a competitive inhibitor of nitrendipine, has no effect on the locomotion of keratocytes. Cell motility was also unaffected by hyperpolarizing and depolarizing (low and high K+) media. It is argued that while a tissue cell may accommodate changes in resting membrane potential without becoming more or less motile, the cell may not be able to counterbalance the effects of depolarization and hyperpolarization simultaneously. In this context, a gradient of membrane potential, which is induced by an external DC electric field, will serve as a persistent stimulus for cell locomotion.

Convergence effect of droplet coalescence under AC and pulsed DC electric fields

2021 ◽  
pp. 103776
Author(s):  
Xin Huang ◽  
Limin He ◽  
Xiaoming Luo ◽  
Ke Xu ◽  
Yuling Lü ◽  
...  
Keyword(s):  
Electric Fields ◽  
Droplet Coalescence ◽  
Pulsed Dc ◽  
Dc Electric Fields

Pulsed DC electric fields couple to natural NAD(P)H oscillations in HT-1080 fibrosarcoma cells

2001 ◽  
Vol 114 (8) ◽  
pp. 1515-1520 ◽  
Author(s):  
A.J. Rosenspire ◽  
A.L. Kindzelskii ◽  
H.R. Petty
Keyword(s):  
Electric Field ◽  
Electric Fields ◽  
Cell Function ◽  
Low Frequency ◽  
Reactive Oxygen Metabolites ◽  
Pulsed Dc ◽  
Fibrosarcoma Cells ◽  
Chemotactic Factors ◽  
Oxygen Metabolites ◽  
Dc Electric Fields

Previously, we have demonstrated that NAD(P)H levels in neutrophils and macrophages are oscillatory. We have also found that weak ultra low frequency AC or pulsed DC electric fields can resonate with, and increase the amplitude of, NAD(P)H oscillations in these cells. For these cells, increased NAD(P)H amplitudes directly signal changes in behavior in the absence of cytokines or chemotactic factors. Here, we have studied the effect of pulsed DC electric fields on HT-1080 fibrosarcoma cells. As in neutrophils and macrophages, NAD(P)H levels oscillate. We find that weak (~10(-)(5) V/m), but properly phased DC (pulsed) electric fields, resonate with NAD(P)H oscillations in polarized and migratory, but not spherical, HT-1080 cells. In this instance, electric field resonance signals an increase in HT-1080 pericellular proteolytic activity. Electric field resonance also triggers an immediate increase in the production of reactive oxygen metabolites. Under resonance conditions, we find evidence of DNA damage in HT-1080 cells in as little as 5 minutes. Thus the ability of external electric fields to effect cell function and physiology by acting on NAD(P)H oscillations is not restricted to cells of the hematopoietic lineage, but may be a universal property of many, if not all polarized and migratory eukaryotic cells.

Charged Dendrimers in Trivalent Salt Solutions under the Action of DC Electric Fields

2014 ◽  
Vol 118 (23) ◽  
pp. 6265-6276 ◽  
Author(s):  
Ashok K. Das ◽  
Pai-Yi Hsiao
Keyword(s):  
Electric Fields ◽  
Salt Solutions ◽  
Dc Electric Fields
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