scholarly journals Mesenchymal stem cell therapies for intervertebral disc degeneration: Consideration of the degenerate niche

JOR Spine ◽  
2019 ◽  
Vol 2 (2) ◽  
Author(s):  
Louise Vickers ◽  
Abbey A. Thorpe ◽  
Joseph Snuggs ◽  
Christopher Sammon ◽  
Christine L. Le Maitre
Keyword(s):  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Intervertebral Disc ◽  
Disc Degeneration ◽  
Intervertebral Disc Degeneration ◽  
Stem Cell Therapies ◽  
Cell Therapies

scholarly journals Bone mesenchymal stem cell-derived extracellular vesicles promote the repair of intervertebral disc degeneration by transferring microRNA-199a

Cell Cycle ◽  
2021 ◽  
Vol 20 (3) ◽  
pp. 256-270
Author(s):  
Tao Wen ◽  
Hongshen Wang ◽  
Yongjin Li ◽  
Yongpeng Lin ◽  
Shuai Zhao ◽  
...  
Keyword(s):  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Intervertebral Disc ◽  
Disc Degeneration ◽  
Extracellular Vesicles ◽  
Intervertebral Disc Degeneration

scholarly journals microRNA-129-5p shuttled by mesenchymal stem cell-derived extracellular vesicles alleviates intervertebral disc degeneration via blockade of LRG1-mediated p38 MAPK activation

2021 ◽  
Vol 12 ◽  
pp. 204173142110216
Author(s):  
Shaoqian Cui ◽  
Lei Zhang
Keyword(s):  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Intervertebral Disc ◽  
Signaling Pathway ◽  
P38 Mapk ◽  
Disc Degeneration ◽  
Extracellular Vesicles ◽  
Intervertebral Disc Degeneration ◽  
Mapk Signaling ◽  
Ecm Degradation

Mesenchymal stem cell (MSC)-derived extracellular vesicles (EVs) have been reported to deliver exogenous microRNAs (miRNAs or miRs) to reduce the progression of intervertebral disc degeneration (IDD). The purpose of the current study was to investigate the therapeutic potential of MSC-derived EVs delivering miR-129-5p in IDD. First, miR-129-5p expression levels were quantified in nucleus pulposus (NP) tissues of IDD patients. An IL-1β-induced NP cell model with IDD was then established, and co-cultured with EVs derived from MSCs that had been transfected with miR-129-5p mimic or inhibitor to elucidate the effects of miR-129-5p on cell viability, apoptosis, and ECM degradation. In addition, RAW264.7 cells were treated with the conditioned medium (CM) of NP cells. Next, the expression patterns of polarization markers and those of inflammatory factors in macrophages were detected using flow cytometry and ELISA, respectively. Lastly, rat models of IDD were established to validate the in vitro findings. It was found that miR-129-5p was poorly-expressed in NP tissues following IDD. Delivery of miR-129-5p to NP cells by MSC-derived EVs brought about a decrease in NP cell apoptosis, ECM degradation and M1 polarization of macrophages. Moreover, miR-129-5p directly-targeted LRG1, which subsequently promoted the activation of p38 MAPK signaling pathway, thus polarizing macrophages toward the M1 phenotype. Furthermore, MSC-derived EVs transferring miR-129-5p relieved IDD via inhibition of the LRG1/p38 MAPK signaling in vivo. Altogether, our findings indicated that MSC-derived EVs carrying miR-129-5p confer protection against IDD by targeting LRG1 and suppressing the p38 MAPK signaling pathway, offering a novel theranostic marker in IDD.

Mesenchymal Stem Cell Injections for Treatment of Intervertebral Disc Degeneration

2008 ◽  
Vol 8 (5) ◽  
pp. 66S-67S
Author(s):  
James D. Kang ◽  
Lars G. Gilbertson ◽  
Gianluca Vadala ◽  
James III Larson
Keyword(s):  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Intervertebral Disc ◽  
Disc Degeneration ◽  
Intervertebral Disc Degeneration

scholarly journals Injectable kartogenin and apocynin loaded micelle enhances the alleviation of intervertebral disc degeneration by adipose-derived stem cell

2021 ◽  
Vol 6 (10) ◽  
pp. 3568-3579
Author(s):  
Chao Yu ◽  
Dongdong Li ◽  
Chenggui Wang ◽  
Kaishun Xia ◽  
Jingkai Wang ◽  
...  
Keyword(s):  
Stem Cell ◽  
Intervertebral Disc ◽  
Disc Degeneration ◽  
Intervertebral Disc Degeneration ◽  
Adipose Derived Stem Cell
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