In Vitro Study of Ethyl‐4‐(3,4.5‐trimethoxyphenyl)‐2,7,7‐trimethyl‐5‐oxo1,4,5,6,7,8‐hexahydroquinoline‐3‐carboxylate and Bovine Serum Albumin Using Multi‐Spectroscopic Techniques and Molecular Docking

2019 ◽  
Vol 387 (1) ◽  
pp. 1800206
Author(s):  
Sunil D. Kumbhar ◽  
Anil H. Gore ◽  
Prafulla B. Choudhari ◽  
Nilotpal Barooah ◽  
Prashant V. Anbhule ◽  
...  
Keyword(s):  
Molecular Docking ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
In Vitro Study ◽  
Vitro Study ◽  
Spectroscopic Techniques

In vitro study on the interaction of Mn(II)-DiAmsar with human serum albumin (HSA) and bovine serum albumin (BSA) by spectroscopic and molecular docking methods

2014 ◽  
Vol 12 (4) ◽  
pp. 715-725 ◽  
Author(s):  
Zari Hooshyar ◽  
Ghasem Rezanejade Bardajee ◽  
Pegah Shafagh ◽  
Samira Ghiasvand ◽  
Mohaddeseh Khanjari ◽  
...  
Keyword(s):  
Molecular Docking ◽  
Human Serum Albumin ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Bovine Serum ◽  
In Vitro Study ◽  
Vitro Study

In Vitro Study of the Binding of Taxifolin to Bovine Serum Albumin and the Influence of Common Ions on the Binding

2010 ◽  
Vol 39 (4) ◽  
pp. 482-494 ◽  
Author(s):  
Xiaolei Shi ◽  
Xuwen Li ◽  
Yantao Sun ◽  
Wei Wei ◽  
Ruijie Yang ◽  
...  
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
In Vitro Study ◽  
Vitro Study

In vitro study of caffeic acid — bovine serum albumin interaction

1988 ◽  
Vol 13 (1) ◽  
pp. 11-14 ◽  
Author(s):  
T. Adzet ◽  
J. Camarasa ◽  
E. Escubedo ◽  
M. Merlos
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Caffeic Acid ◽  
Bovine Serum ◽  
In Vitro Study ◽  
Vitro Study

Synthesis, Molecular Docking, BSA, and in-vitro reactivation study of imidazopyridine oxime against paraoxon inhibited acetylcholinesterase

2021 ◽  
Vol 17 ◽  
Author(s):  
Ashima Thakur ◽  
Jayant Patwa ◽  
Abha Sharma ◽  
Swaran Jeet Flora
Keyword(s):  
Molecular Docking ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
In Silico ◽  
Bovine Serum ◽  
Enzymatic Assay ◽  
Acid Dissociation ◽  
Acid Dissociation Constant ◽  
Spectroscopic Techniques

Aim: To synthesize and evaluate the fused heterocyclic imidazopyridine oxime as a reactivator against paraoxon inhibited acetylcholinesterase. Background: Organophosphorus compounds (OPs) include parathion, malathion, chlorpyrifos, monocrotophos, and diazinon which are commonly used in agriculture for enhancing agricultural productivity via killing crop-damaging pests. However, people may get exposed to OPs pesticides unintentionally/intentionally via ingestion, inhalation or dermal. The current treatment regimen includes reactivator such as mono or bis-pyridinium oximes along with anticholinergic and an anticonvulsant drugs are recommended for the treatment of OP poisoning. Unfortunately, the drawback of the existing reactivator is that owing to the permanent charge present on the pyridinium makes them inefficient to cross the blood-brain barrier (BBB) and reactivate OP-inhibited central nervous system (CNS) acetylcholinesterase. Therefore, there is a need of reactivator that could cross the BBB and reactivate the OP inhibited acetylcholinesterase. Objective: The objectives of the study were synthesis, molecular docking, BSA binding and in-vitro estimation of oximes of various substituted imidazo [1,2-a]pyridine against paraoxon inhibited acetylcholinesterase. Method: The reactivators were synthesized in three steps and characterized using various spectroscopic techniques. Molecular docking study was performed on 2WHP and 3ZLV PDB using Autodock tool. The acid dissociation constant (pKa) of oximes was calculated experimentally and drug-likeness properties of the oximes were calculated In silico using mole inspiration and Swiss ADME software. The binding of oximes with bovine serum albumin (BSA) was also investigated by UV-Vis spectrophotometer. The reactivation potential of the oximes was determined by in vitro enzymatic assay. Result: in-silico study inferred that synthesized molecules fulfilled the parameters that required for a successful CNS drug candidate. Further, in-vitro enzymatic assay indicated reasonable reactivation potential of the oximes against paraoxon-inhibited AChE. The binding of oximes with bovine serum albumin (BSA) revealed static quenching of intrinsic fluorescence of BSA by oxime. The binding constant value and number of binding sites were found 0.24 mol-1 and 1 respectively. Conclusion: The results of study concluded that this scaffold could be used for further designing of more efficient uncharged reactivators.

In vitro study on the binding of gemcitabine to bovine serum albumin

2013 ◽  
Vol 75 ◽  
pp. 86-93 ◽  
Author(s):  
Haibo Shen ◽  
Zhengqin Gu ◽  
Kang Jian ◽  
Juan Qi
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
In Vitro Study ◽  
Vitro Study

Sulfadiazine binds and unfolds bovine serum albumin: an in vitro study

2013 ◽  
Vol 40 (11) ◽  
pp. 6081-6090 ◽  
Author(s):  
Mohd. Sajid Ali ◽  
Hamad A. Al-Lohedan
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
In Vitro Study ◽  
Vitro Study
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