Electron spin resonance (ESR/EPR) of free radicals observed in human red hair: a new, simple empirical method of determination of pheomelanin/eumelanin ratio in hair

2014 ◽  
Vol 52 (7) ◽  
pp. 377-382 ◽  
Author(s):  
Eduard N. Chikvaidze ◽  
Tamar M. Partskhaladze ◽  
Temur V. Gogoladze
Keyword(s):  
Free Radicals ◽  
Electron Spin Resonance ◽  
Electron Spin ◽  
Empirical Method ◽  
Red Hair ◽  
Method Of Determination ◽  
Spin Resonance

Determination of free radicals in polymer films by electron spin resonance spectrometry

1983 ◽  
Vol 55 (9) ◽  
pp. 1520-1522 ◽  
Author(s):  
John L. Gerlock
Keyword(s):  
Free Radicals ◽  
Electron Spin Resonance ◽  
Electron Spin ◽  
Polymer Films ◽  
Spin Resonance

scholarly journals Estimation of the Postmortem Duration of Mouse Tissue by Electron Spin Resonance Spectroscopy

2011 ◽  
Vol 2011 ◽  
pp. 1-11
Author(s):  
Shinobu Ito ◽  
Tomohisa Mori ◽  
Hideko Kanazawa ◽  
Toshiko Sawaguchi
Keyword(s):  
Free Radicals ◽  
Electron Spin Resonance ◽  
Electron Spin ◽  
Spin Trap ◽  
Detection Sensitivity ◽  
Mouse Tissue ◽  
Spin Adduct ◽  
Simple Method ◽  
Oxidation Stress ◽  
Spin Resonance

Electron spin resonance (ESR) method is a simple method for detecting various free radicals simultaneously and directly. However, ESR spin trap method is unsuited to analyze weak ESR signals in organs because of water-induced dielectric loss (WIDL). To minimize WIDL occurring in biotissues and to improve detection sensitivity to free radicals in tissues, ESR cuvette was modified and used with 5,5-dimethtyl-1-pyrroline N-oxide (DMPO). The tissue samples were mouse brain, hart, lung, liver, kidney, pancreas, muscle, skin, and whole blood, where various ESR spin adduct signals including DMPO-ascorbyl radical (AsA∗), DMPO-superoxide anion radical (OOH), and DMPO-hydrogen radical (H) signal were detected. Postmortem changes in DMPO-AsA∗and DMPO-OOH were observed in various tissues of mouse. The signal peak of spin adduct was monitored until the 205th day postmortem. DMPO-AsA∗in liver (y=113.8–40.7 log (day),R1=-0.779,R2=0.6,P<.001) was found to linearly decrease with the logarithm of postmortem duration days. Therefore, DMPO-AsA∗signal may be suitable for detecting an oxidation stress tracer from tissue in comparison with other spin adduct signal on ESR spin trap method.

Free Radicals in Wheat Seeds Studied by Electron Spin Resonance

2002 ◽  
Vol 67 (6) ◽  
pp. 2079-2082 ◽  
Author(s):  
F. Szocs
Keyword(s):  
Free Radicals ◽  
Electron Spin Resonance ◽  
Electron Spin ◽  
Spin Resonance ◽  
Wheat Seeds
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