Testicular stem cells for fertility preservation: Preclinical studies on male germ cell transplantation and testicular grafting

2009 ◽  
Vol 53 (2) ◽  
pp. 274-280 ◽  
Author(s):  
Stefan Schlatt ◽  
Jens Ehmcke ◽  
Kirsi Jahnukainen
Keyword(s):  
Stem Cells ◽  
Germ Cell ◽  
Fertility Preservation ◽  
Cell Transplantation ◽  
Male Germ Cell ◽  
Preclinical Studies ◽  
Germ Cell Transplantation

scholarly journals Male germ cell transplantation in livestock

2006 ◽  
Vol 18 (2) ◽  
pp. 13 ◽  
Author(s):  
J. R. Hill ◽  
I. Dobrinski
Keyword(s):  
Stem Cells ◽  
Germ Cell ◽  
Cell Transplantation ◽  
Large Scale ◽  
Developmental State ◽  
Male Germ Cell ◽  
Seminiferous Tubules ◽  
Cell Transfer ◽  
Donor Sperm ◽  
Germ Cell Transplantation

Male germ cell transplantation is a powerful approach to study the control of spermatogenesis with the ultimate goal to enhance or suppress male fertility. In livestock animals, applications can be expanded to provide an alternative method of transgenesis and an alternative means of artificial insemination (AI). The transplantation technique uses testis stem cells, harvested from the donor animal. These donor stem cells are injected into seminiferous tubules, migrate from the lumen to relocate to the basement membrane and, amazingly, they can retain the capability to produce donor sperm in their new host. Adaptation of the mouse technique for livestock is progressing, with gradual gains in efficiency. Germ cell transfer in goats has produced offspring, but not yet in cattle and pigs. In goats and pigs, the applications of germ cell transplantation are mainly in facilitating transgenic animal production. In cattle, successful male germ cell transfer could create an alternative to AI in areas where it is impractical. Large-scale culture of testis stem cells would enhance the use of elite bulls by providing a renewable source of stem cells for transfer. Although still in a developmental state, germ cell transplantation is an emerging technology with the potential to create new opportunities in livestock production.

scholarly journals Successful Intra- and Interspecific Male Germ Cell Transplantation in the Rat1

2003 ◽  
Vol 68 (3) ◽  
pp. 961-967 ◽  
Author(s):  
Zhen Zhang ◽  
Marilyn B. Renfree ◽  
Roger V. Short
Keyword(s):  
Germ Cell ◽  
Cell Transplantation ◽  
Male Germ Cell ◽  
Germ Cell Transplantation

Male germ cell transplantation: promise and problems

2001 ◽  
Vol 13 (8) ◽  
pp. 609 ◽  
Author(s):  
Fang-Xu Jiang
Keyword(s):  
Germ Cell ◽  
Cell Transplantation ◽  
Germ Cells ◽  
Cell Biology ◽  
Direct Injection ◽  
Primordial Germ Cells ◽  
Rete Testis ◽  
Male Germ Cell ◽  
Seminiferous Tubules ◽  
Germ Cell Transplantation

Male germ cell transplantation is a novel technique in which donor male stem germ cells are surgically transferred to the seminiferous tubules of a recipient testis by direct injection or via the rete testis or efferent duct. All germ cells that are destined to become stem spermatogonia are defined as male stem germ cells, including primordial germ cells from the gonadal ridges, and gonocytes and stem spermatogonia from the testis, all of which are transplantable and capable of undergoing normal spermatogenesis. Xenotransplantation of male germ cells from one species into the testis of another species, including human testicular cells in the mouse, has so far proved to be unsuccessful. However, the immunodeficient mouse testis can support rat spermatogenesis and produce apparently normal rat spermatozoa. The underlying mechanisms remain elusive. The present mini-review will focus on the importance of stem spermatogonial transplantation for testicular stem cell biology and discuss the likelihood of immune rejection after transplantation, which may limit the success of all male germ cell transplantation.

Adeno‐associated virus (AAV)‐mediated transduction of male germ line stem cells results in transgene transmission after germ cell transplantation

2007 ◽  
Vol 22 (2) ◽  
pp. 374-382 ◽  
Author(s):  
Ali Honaramooz ◽  
Susan Megee ◽  
Wenxian Zeng ◽  
Margret M. Destrempes ◽  
Susan A. Overton ◽  
...  
Keyword(s):  
Stem Cells ◽  
Germ Cell ◽  
Cell Transplantation ◽  
Germ Line ◽  
Male Germ Line ◽  
Adeno Associated Virus ◽  
Germ Cell Transplantation ◽  
Transgene Transmission
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