scholarly journals Non-viral transfection of goat germline stem cells by nucleofection results in production of transgenic sperm after germ cell transplantation

2012 ◽  
Vol 79 (4) ◽  
pp. 255-261 ◽  
Author(s):  
W. Zeng ◽  
L. Tang ◽  
A. Bondareva ◽  
J. Luo ◽  
S.O. Megee ◽  
...  
Keyword(s):  
Stem Cells ◽  
Germ Cell ◽  
Cell Transplantation ◽  
Germline Stem Cells ◽  
Germ Cell Transplantation ◽  
Viral Transfection

scholarly journals Viral Transduction of Male Germline Stem Cells Results in Transgene Transmission after Germ Cell Transplantation in Pigs1

2013 ◽  
Vol 88 (1) ◽  
Author(s):  
Wenxian Zeng ◽  
Lin Tang ◽  
Alla Bondareva ◽  
Ali Honaramooz ◽  
Valeria Tanco ◽  
...  
Keyword(s):  
Stem Cells ◽  
Germ Cell ◽  
Cell Transplantation ◽  
Germline Stem Cells ◽  
Male Germline ◽  
Germ Cell Transplantation ◽  
Viral Transduction ◽  
Male Germline Stem Cells ◽  
Transgene Transmission

Testicular stem cells for fertility preservation: Preclinical studies on male germ cell transplantation and testicular grafting

2009 ◽  
Vol 53 (2) ◽  
pp. 274-280 ◽  
Author(s):  
Stefan Schlatt ◽  
Jens Ehmcke ◽  
Kirsi Jahnukainen
Keyword(s):  
Stem Cells ◽  
Germ Cell ◽  
Fertility Preservation ◽  
Cell Transplantation ◽  
Male Germ Cell ◽  
Preclinical Studies ◽  
Germ Cell Transplantation

scholarly journals Male germ cell transplantation in livestock

2006 ◽  
Vol 18 (2) ◽  
pp. 13 ◽  
Author(s):  
J. R. Hill ◽  
I. Dobrinski
Keyword(s):  
Stem Cells ◽  
Germ Cell ◽  
Cell Transplantation ◽  
Large Scale ◽  
Developmental State ◽  
Male Germ Cell ◽  
Seminiferous Tubules ◽  
Cell Transfer ◽  
Donor Sperm ◽  
Germ Cell Transplantation

Male germ cell transplantation is a powerful approach to study the control of spermatogenesis with the ultimate goal to enhance or suppress male fertility. In livestock animals, applications can be expanded to provide an alternative method of transgenesis and an alternative means of artificial insemination (AI). The transplantation technique uses testis stem cells, harvested from the donor animal. These donor stem cells are injected into seminiferous tubules, migrate from the lumen to relocate to the basement membrane and, amazingly, they can retain the capability to produce donor sperm in their new host. Adaptation of the mouse technique for livestock is progressing, with gradual gains in efficiency. Germ cell transfer in goats has produced offspring, but not yet in cattle and pigs. In goats and pigs, the applications of germ cell transplantation are mainly in facilitating transgenic animal production. In cattle, successful male germ cell transfer could create an alternative to AI in areas where it is impractical. Large-scale culture of testis stem cells would enhance the use of elite bulls by providing a renewable source of stem cells for transfer. Although still in a developmental state, germ cell transplantation is an emerging technology with the potential to create new opportunities in livestock production.

Adeno‐associated virus (AAV)‐mediated transduction of male germ line stem cells results in transgene transmission after germ cell transplantation

2007 ◽  
Vol 22 (2) ◽  
pp. 374-382 ◽  
Author(s):  
Ali Honaramooz ◽  
Susan Megee ◽  
Wenxian Zeng ◽  
Margret M. Destrempes ◽  
Susan A. Overton ◽  
...  
Keyword(s):  
Stem Cells ◽  
Germ Cell ◽  
Cell Transplantation ◽  
Germ Line ◽  
Male Germ Line ◽  
Adeno Associated Virus ◽  
Germ Cell Transplantation ◽  
Transgene Transmission

scholarly journals Aging- and temperature-related activity of spermatogonial stem cells for germ cell transplantation in medaka

2020 ◽  
Vol 155 ◽  
pp. 213-221
Author(s):  
Rungsun Duangkaew ◽  
Fumi Kezuka ◽  
Kensuke Ichida ◽  
Surintorn Boonanuntanasarn ◽  
Goro Yoshizaki
Keyword(s):  
Stem Cells ◽  
Germ Cell ◽  
Cell Transplantation ◽  
Spermatogonial Stem Cells ◽  
Related Activity ◽  
Germ Cell Transplantation

scholarly journals 022.Spermatogonial stem cells: from basic research to clinical applications

2004 ◽  
Vol 16 (9) ◽  
pp. 22
Author(s):  
S. Schlatt
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Germ Cell ◽  
Cell Transplantation ◽  
Basic Research ◽  
Testicular Tissue ◽  
Spermatogonial Stem Cells ◽  
Immunodeficient Mice ◽  
Germ Cell Transplantation ◽  
Oncological Patients

The testis contains undifferentiated spermatogonia and is therefore the only adult organ populated with proliferating germline cells. Whereas the biology of these cells is quite well understood in rodents, their modes of mitotic expansion and differentiation are poorly understood in primates. The existence of these cells offers clinically relevant options for preservation and restoration of male fertility. New approaches based on male germ cell transplantation and testicular tissue grafting can be applied to generate a limited number of sperm and could therefore be considered as important new avenues applicable to a variety of disciplines like animal conservation, genetic germline modification or restoration of fertility in oncological patients. In principle, germ cell transplantation presents a removal of the stem cell from the donor's niche and a transfer into the niche of a recipient. Grafting can be considered as a transplantation of the stem cell in conjunction with its niche. Germ cell transplantation of human spermatogonia into mouse testes revealed that the stem cells survive and expand but are not able to differentiate and complete spermatogenesis. We have developed an approach to infuse germ cells into monkey and human testes and showed that germ cell transplantation is feasible as an autologous approach in primates. Furthermore, we applied germ cell transplantation in the monkey model mimicking a gonadal protection strategy for oncological patients. Ectopic xenografting of testicular tissue was applied to generate fertile sperm from a variety of species. Newborn testicular tissue was grafted into the back skin of immunodeficient mice and developed up to qualitatively complete spermatogenesis. The rapid progress in the development of novel experimental strategies to generate sperm from cryopreserved spermatogonial stem cells or immature testicular tissue will lead to many new options for germline manipulation and fertility preservation.

scholarly journals Spermatogonial stem cells from domestic animals: progress and prospects

Reproduction ◽  
2014 ◽  
Vol 147 (3) ◽  
pp. R65-R74 ◽  
Author(s):  
Yi Zheng ◽  
Yaqing Zhang ◽  
Rongfeng Qu ◽  
Ying He ◽  
Xiue Tian ◽  
...  
Keyword(s):  
Stem Cells ◽  
Germ Cell ◽  
Cell Transplantation ◽  
Pluripotent Stem Cells ◽  
Ethical Issues ◽  
Embryonic Stem ◽  
Domestic Animals ◽  
Spermatogonial Stem Cells ◽  
Germ Cell Transplantation

Spermatogenesis, an elaborate and male-specific process in adult testes by which a number of spermatozoa are produced constantly for male fertility, relies on spermatogonial stem cells (SSCs). As a sub-population of undifferentiated spermatogonia, SSCs are capable of both self-renewal (to maintain sufficient quantities) and differentiation into mature spermatozoa. SSCs are able to convert to pluripotent stem cells during in vitro culture, thus they could function as substitutes for human embryonic stem cells without ethical issues. In addition, this process does not require exogenous transcription factors necessary to produce induced-pluripotent stem cells from somatic cells. Moreover, combining genetic engineering with germ cell transplantation would greatly facilitate the generation of transgenic animals. Since germ cell transplantation into infertile recipient testes was first established in 1994, in vivo and in vitro study and manipulation of SSCs in rodent testes have been progressing at a staggering rate. By contrast, their counterparts in domestic animals, despite the failure to reach a comparable level, still burgeoned and showed striking advances. This review outlines the recent progressions of characterization, isolation, in vitro propagation, and transplantation of spermatogonia/SSCs from domestic animals, thereby shedding light on future exploration of these cells with high value, as well as contributing to the development of reproductive technology for large animals.

Germ cell transplantation for the propagation of companion animals, non-domestic and endangered species

2007 ◽  
Vol 19 (6) ◽  
pp. 732 ◽  
Author(s):  
I. Dobrinski ◽  
A. J. Travis
Keyword(s):  
Stem Cells ◽  
Germ Cell ◽  
Cell Transplantation ◽  
Germ Line ◽  
Genetic Manipulation ◽  
Mammalian Species ◽  
Companion Animals ◽  
Reproductive Potential ◽  
Embryonic Stem ◽  
Germ Cell Transplantation

The transplantation of spermatogonial stem cells between males results in a recipient animal producing spermatozoa carrying a donor’s haplotype. First pioneered in rodents, this technique has now been used in several animal species. Importantly, germ cell transplantation was successful between unrelated, immuno-competent large animals, whereas efficient donor-derived spermatogenesis in rodents requires syngeneic or immuno-compromised recipients. Transplantation requires four steps: recipient preparation, donor cell isolation, transplantation and identifying donor-derived spermatozoa. There are two main applications for this technology. First, genetic manipulation of isolated germ line stem cells and subsequent transplantation will result in production of transgenic spermatozoa. Transgenesis through the male germ line has tremendous potential in species in which embryonic stem cells are not available and somatic cell nuclear transfer and reprogramming pose several problems. Second, spermatogonial stem cell transplantation within or between species offers a means of preserving the reproductive potential of genetically valuable individuals. This might have significance in the captive propagation of non-domestic animals of high conservation value. Transplantation of germ cells is a uniquely valuable approach for the study, preservation and manipulation of male fertility in mammalian species.

scholarly journals Induced sterility in fish and its potential and challenges for aquaculture and germ cell transplantation technology: a review

Biologia ◽  
2016 ◽  
Vol 71 (8) ◽  
Author(s):  
Amin Golpour ◽  
Mohammad Abdul Momin Siddique ◽  
Diógenes Henrique Siqueira-Silva ◽  
Martin Pšenička
Keyword(s):  
Germ Cell ◽  
Cell Transplantation ◽  
Large Scale ◽  
Hormonal Treatment ◽  
Important Species ◽  
Genetic Ablation ◽  
Germ Cell Transplantation ◽  
Commercially Important ◽  
Alternative Approaches ◽  
Hormone Signalling

AbstractInterest in reproductively sterile fish in aquaculture has prompted research into their production. Several methods are available for inducing sterility and optimizing its application in the global fishery industry. Sterilization can potentially be accomplished through irradiation, surgery, or chemical and hormonal treatment. Alternative approaches include triploidization, hybridization, and generation of new lines via advanced biotechnological techniques. Triploids of many commercially important species have been studied extensively and have been produced on a large scale for many years. Novel approaches, including disruption of gonadotropin releasing hormone signalling and genetic ablation of germ cells, have been developed that are effective in producing infertile fish but have the disadvantage of not being 100% reliable or are impractical for large-scale aquaculture. We review currently used technologies and recent advances in induction of sterility in fish, especially those intended for use in germ cell transplantation. Knowledge of the implications of these approaches remains incomplete, imposing considerable limitations.

scholarly journals Restoration of Fertility by Germ Cell Transplantation Requires Effective Recipient Preparation1

2003 ◽  
Vol 69 (2) ◽  
pp. 412-420 ◽  
Author(s):  
Clayton J. Brinster ◽  
Buom-Yong Ryu ◽  
Mary R. Avarbock ◽  
Levent Karagenc ◽  
Ralph L. Brinster ◽  
...  
Keyword(s):  
Germ Cell ◽  
Cell Transplantation ◽  
Germ Cell Transplantation ◽  
Restoration Of Fertility
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