scholarly journals Applications of shear-induced polarized light imaging (SIPLI) technique for mechano-optical rheology of polymers and soft matter materials

2016 ◽  
Vol 54 (21) ◽  
pp. 2151-2170 ◽  
Author(s):  
Oleksandr O. Mykhaylyk ◽  
Nicholas J. Warren ◽  
Andrew J. Parnell ◽  
Gerhard Pfeifer ◽  
Joerg Laeuger
Keyword(s):  
Soft Matter ◽  
Polarized Light ◽  
Polarized Light Imaging

scholarly journals Kinetochore-driven outgrowth of microtubules is a central contributor to kinetochore fiber maturation in crane-fly spermatocytes

2014 ◽  
Vol 25 (9) ◽  
pp. 1437-1445 ◽  
Author(s):  
James R. LaFountain ◽  
Rudolf Oldenbourg
Keyword(s):  
Liquid Crystal ◽  
Life Histories ◽  
Polarized Light ◽  
Fiber Formation ◽  
Image Brightness ◽  
Early Stages ◽  
Anaphase Onset ◽  
Bipolar Spindles ◽  
Polarized Light Imaging

We use liquid crystal polarized light imaging to record the life histories of single kinetochore (K-) fibers in living crane-fly spermatocytes, from their origins as nascent K-fibers in early prometaphase to their fully matured form at metaphase, just before anaphase onset. Increased image brightness due to increased retardance reveals where microtubules are added during K-fiber formation. Analysis of experimentally generated bipolar spindles with only one centrosome, as well as of regular, bicentrosomal spindles, reveals that microtubule addition occurs at the kinetochore-proximal ends of K-fibers, and added polymer expands poleward, giving rise to the robust K-fibers of metaphase cells. These results are not compatible with a model for K-fiber formation in which microtubules are added to nascent fibers solely by repetitive “search and capture” of centrosomal microtubule plus ends. Our interpretation is that capture of centrosomal microtubules—when deployed—is limited to early stages in establishment of nascent K-fibers, which then mature through kinetochore-driven outgrowth. When kinetochore capture of centrosomal microtubules is not used, the polar ends of K-fibers grow outward from their kinetochores and usually converge to make a centrosome-free pole.

Quantitative comparison of human myocardial fiber orientations derived from DTI and polarized light imaging

2018 ◽  
Vol 63 (21) ◽  
pp. 215003 ◽  
Author(s):  
Feng Yang ◽  
Yue-Min Zhu ◽  
Gabrielle Michalowicz ◽  
Pierre-Simon Jouk ◽  
Laurent Fanton ◽  
...  
Keyword(s):  
Polarized Light ◽  
Quantitative Comparison ◽  
Myocardial Fiber ◽  
Polarized Light Imaging

scholarly journals Detecting axial heterogeneity of birefringence in layered turbid media using polarized light imaging

2012 ◽  
Vol 3 (12) ◽  
pp. 3250 ◽  
Author(s):  
Sanaz Alali ◽  
Yuting Wang ◽  
I. Alex Vitkin
Keyword(s):  
Polarized Light ◽  
Turbid Media ◽  
Polarized Light Imaging

Grazing incidence polarized light imaging of footwear prints

2019 ◽  
Vol 20 (11) ◽  
pp. 1543-1550
Author(s):  
Xin-yi Bi ◽  
Rui-fang Han ◽  
Ran Liao ◽  
Wu-sheng Feng ◽  
Da Li ◽  
...  
Keyword(s):  
Polarized Light ◽  
Grazing Incidence ◽  
Polarized Light Imaging

scholarly journals Analytical Fiber ODF Reconstruction in 3D Polarized Light Imaging: Performance Assessment

2019 ◽  
Author(s):  
A. Alimi ◽  
S. Deslauriers-Gauthier ◽  
F. Matuschke ◽  
D. Schmitz ◽  
M. Axer ◽  
...  
Keyword(s):  
Performance Assessment ◽  
Polarized Light ◽  
Imaging Performance ◽  
Polarized Light Imaging

scholarly journals Evaluation of collimated polarized light imaging for real-time intraoperative selective nerve identification in the human hand

2017 ◽  
Vol 8 (9) ◽  
pp. 4122 ◽  
Author(s):  
K. W. T. K Chin ◽  
A. F. Engelsman ◽  
P. T. K. Chin ◽  
S. L. Meijer ◽  
S. D. Strackee ◽  
...  
Keyword(s):  
Real Time ◽  
Polarized Light ◽  
Human Hand ◽  
Nerve Identification ◽  
Polarized Light Imaging

scholarly journals Rigid and non-rigid registration of polarized light imaging data for 3D reconstruction of the temporal lobe of the human brain at micrometer resolution

NeuroImage ◽  
2018 ◽  
Vol 181 ◽  
pp. 235-251 ◽  
Author(s):  
Sharib Ali ◽  
Stefan Wörz ◽  
Katrin Amunts ◽  
Roland Eils ◽  
Markus Axer ◽  
...  
Keyword(s):  
3D Reconstruction ◽  
Human Brain ◽  
Temporal Lobe ◽  
Polarized Light ◽  
Imaging Data ◽  
Rigid Registration ◽  
Polarized Light Imaging

Polarized light imaging of object hidden in scattering media: model experiments

2001 ◽  
Author(s):  
Yurii P. Sinichkin ◽  
Dmitry A. Zimnyakov ◽  
Vadim V. Giterman
Keyword(s):  
Polarized Light ◽  
Scattering Media ◽  
Model Experiments ◽  
Polarized Light Imaging

scholarly journals A Jones matrix formalism for simulating three-dimensional polarized light imaging of brain tissue

2015 ◽  
Vol 12 (111) ◽  
pp. 20150734 ◽  
Author(s):  
M. Menzel ◽  
K. Michielsen ◽  
H. De Raedt ◽  
J. Reckfort ◽  
K. Amunts ◽  
...  
Keyword(s):  
Myelin Sheath ◽  
Experimental Studies ◽  
Three Dimensional ◽  
Polarized Light ◽  
Microscopic Model ◽  
Macroscopic Model ◽  
Jones Matrix ◽  
Matrix Formalism ◽  
Nerve Fibres ◽  
Polarized Light Imaging

The neuroimaging technique three-dimensional polarized light imaging (3D-PLI) provides a high-resolution reconstruction of nerve fibres in human post-mortem brains. The orientations of the fibres are derived from birefringence measurements of histological brain sections assuming that the nerve fibres—consisting of an axon and a surrounding myelin sheath—are uniaxial birefringent and that the measured optic axis is oriented in the direction of the nerve fibres (macroscopic model). Although experimental studies support this assumption, the molecular structure of the myelin sheath suggests that the birefringence of a nerve fibre can be described more precisely by multiple optic axes oriented radially around the fibre axis (microscopic model). In this paper, we compare the use of the macroscopic and the microscopic model for simulating 3D-PLI by means of the Jones matrix formalism. The simulations show that the macroscopic model ensures a reliable estimation of the fibre orientations as long as the polarimeter does not resolve structures smaller than the diameter of single fibres. In the case of fibre bundles, polarimeters with even higher resolutions can be used without losing reliability. When taking the myelin density into account, the derived fibre orientations are considerably improved.

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