Humic acid induces the endothelial nitric oxide synthase phosphorylation at Ser1177 and Thr495 Via Hsp90α and Hsp90β upregulation in human umbilical vein endothelial cells

2013 ◽  
Vol 30 (2) ◽  
pp. 223-231 ◽  
Author(s):  
Masato Tanaka ◽  
Miki Miyajima ◽  
Naoko Hishioka ◽  
Ryo Nishimura ◽  
Yusuke Kihara ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Endothelial Cells ◽  
Humic Acid ◽  
Nitric Oxide Synthase ◽  
Endothelial Nitric Oxide Synthase ◽  
Umbilical Vein ◽  
Human Umbilical Vein ◽  
Umbilical Vein Endothelial Cells ◽  
Oxide Synthase ◽  
Endothelial Nitric Oxide

Glycated human serum albumin induces NF-κB activation and endothelial nitric oxide synthase uncoupling in human umbilical vein endothelial cells

2015 ◽  
Vol 29 (8) ◽  
pp. 984-992 ◽  
Author(s):  
Bruno K. Rodiño-Janeiro ◽  
Beatriz Paradela-Dobarro ◽  
Sergio Raposeiras-Roubín ◽  
Mercedes González-Peteiro ◽  
José R. González-Juanatey ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Endothelial Cells ◽  
Nitric Oxide Synthase ◽  
Human Serum Albumin ◽  
Serum Albumin ◽  
Endothelial Nitric Oxide Synthase ◽  
Umbilical Vein ◽  
Umbilical Vein Endothelial Cells ◽  
Oxide Synthase ◽  
Endothelial Nitric Oxide

Polychlorinated biphenyls alter the expression of endothelial nitric oxide synthase mRNA in human umbilical vein endothelial cells

2007 ◽  
Vol 26 (10) ◽  
pp. 811-816 ◽  
Author(s):  
Naoko Omori ◽  
Hideki Fukata ◽  
Koji Sato ◽  
Koji Yamazaki ◽  
Keiko Aida-Yasuoka ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Endothelial Cells ◽  
Nitric Oxide Synthase ◽  
Polychlorinated Biphenyls ◽  
Endothelial Nitric Oxide Synthase ◽  
Umbilical Vein ◽  
Enos Mrna ◽  
Umbilical Vein Endothelial Cells ◽  
Oxide Synthase ◽  
Endothelial Nitric Oxide

Polychlorinated biphenyls (PCBs) are a group of persistent pollutants that are detected in maternal serum and umbilical cord, suggesting that fetal exposure also needs to be considered. The effects of dioxin-like PCB congeners 3,3',4,4'-tetrachlorobiphenyl (PCB77) and 3,3',4,4',5-pentachlorobiphenyl (PCB126) and a non-dioxin-like compound 2,2',4,4',5,5'-hexachlorobiphenyl (PCB153) on the expression of endothelial nitric oxide synthase (eNOS), known to maintain blood flow to the fetus, in human umbilical vein endothelial cells (HUVECs) were investigated. The mRNA levels of eNOS, aryl hydrocarbon receptor (AhR) and cytochrome P450 (CYP) 1A1 in cells treated with 5 µM PCBs for 24 hours were analysed by real-time RT-PCR. Cells were also treated with α-naphthoflavone (α NF), an AhR antagonist or ICI 182780, an estrogen receptor (ER) antagonist, one hour prior to PCB exposure, to observe the effects of these receptors on eNOS modulation. Each PCB increased the eNOS mRNA level by 4.5-fold that was markedly inhibited by αNF. ERs were also suspected of altering eNOS levels because ICI 182780 treatment resulted in a decrease in the eNOS level. These results suggest that the eNOS mRNA expression increases due to the action of PCBs related to both AhR and ERs in HUVECs, and that maternal PCB exposure could influence fetal circulation. Human & Experimental Toxicology (2007) 26, 811— 816

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