Purification and Characterization of Protein Kinase C from a Higher Plant, Brassica campestris L

1994 ◽  
Vol 203 (1) ◽  
pp. 311-318 ◽  
Author(s):  
T. Nanmori ◽  
W. Taguchi ◽  
M. Kinugasa ◽  
Y. Oji ◽  
S. Sahara ◽  
...  
Keyword(s):  
Protein Kinase C ◽  
Protein Kinase ◽  
Brassica Campestris ◽  
Purification And Characterization ◽  
Higher Plant ◽  
Kinase C

scholarly journals Purification and characterization of Ca2+phospholipid dependent protein kinase (C kinase) from human platelets.

1985 ◽  
Vol 16 (6) ◽  
pp. 614-617
Author(s):  
Masakatsu NISHIKAWA ◽  
Hiroyoshi HIDAKA ◽  
Shigeru SHIRAKAWA ◽  
Robert S. ADELSTEIN
Keyword(s):  
Protein Kinase C ◽  
Protein Kinase ◽  
Human Platelets ◽  
Purification And Characterization ◽  
Dependent Protein Kinase ◽  
Kinase C ◽  
Dependent Protein

scholarly journals Purification and characterization of protein kinase C from the nematode Caenorhabditis elegans

1992 ◽  
Vol 282 (1) ◽  
pp. 219-223 ◽  
Author(s):  
T Sassa ◽  
J Miwa
Keyword(s):  
Protein Kinase C ◽  
Caenorhabditis Elegans ◽  
Protein Kinase ◽  
Column Chromatography ◽  
Purification And Characterization ◽  
C Elegans ◽  
Kinase C ◽  
Sds Page ◽  
Cytosolic Extract

Protein kinase C (PKC) of Caenorhabditis elegans was identified by enzymatic activity and [3H]phorbol 12,13-dibutyrate binding after DEAE-Sephacel column chromatography of a crude cytosolic extract. Ca(2+)-dependent activation of nematode PKC was observed in the presence of phosphatidylserine. The enzyme was maximally activated by 1,2-dioleoylglycerol or phorbol 12-myristate 13-acetate in the presence of phosphatidylserine and Ca2+. Hydroxyapatite column chromatography showed only one peak of PKC activity with histone H1 and myelin basic protein as substrates. The enzyme was purified to near homogeneity by sequential chromatography on polylysine-agarose and phosphatidylserine affinity columns. The purified protein showed a molecular mass of 79 kDa on SDS/PAGE. The substrate specificity of the C. elegans enzyme was shown to be different from that of mammalian PKCs. Here we describe some of the properties of the nematode enzyme.

Purification and characterization of protein kinase C .epsilon. from rabbit brain

Biochemistry ◽  
1992 ◽  
Vol 31 (2) ◽  
pp. 482-490 ◽  
Author(s):  
Takaomi C. Saido ◽  
Keiko Mizuno ◽  
Yasuhiko Konno ◽  
Shinichi Osada ◽  
Shigeo Ohno ◽  
...  
Keyword(s):  
Protein Kinase C ◽  
Protein Kinase ◽  
Rabbit Brain ◽  
Purification And Characterization ◽  
Kinase C ◽  
Protein Kinase C Epsilon

Purification and Characterization of Receptors for Activated Protein Kinase C from Rat Hepatocytes

1997 ◽  
Vol 10 (1) ◽  
pp. 32-37 ◽  
Author(s):  
Martha Robles-Flores ◽  
Erika Rendón-Huerta ◽  
J.Adolfo Garcı́a-Sáinz
Keyword(s):  
Protein Kinase C ◽  
Protein Kinase ◽  
Rat Hepatocytes ◽  
Purification And Characterization ◽  
Kinase C
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