scholarly journals Embryonic Stem Cells and Transgenic Mice Ubiquitously Expressing a Tau-Tagged Green Fluorescent Protein

2000 ◽  
Vol 228 (1) ◽  
pp. 19-28 ◽  
Author(s):  
Thomas Pratt ◽  
Linda Sharp ◽  
Jenny Nichols ◽  
David J. Price ◽  
John O. Mason
Keyword(s):  
Stem Cells ◽  
Green Fluorescent Protein ◽  
Embryonic Stem Cells ◽  
Transgenic Mice ◽  
Fluorescent Protein ◽  
Embryonic Stem ◽  
Green Fluorescent

Generation of Green Fluorescent Protein-Expressing Monkey Embryonic Stem Cells

2006 ◽  
pp. 305-312
Author(s):  
Yutaka Suzuki ◽  
Tatsuyuki Takada ◽  
Nae Kadota ◽  
Yasushi Kondo ◽  
Ryuzo Torii
Keyword(s):  
Stem Cells ◽  
Green Fluorescent Protein ◽  
Embryonic Stem Cells ◽  
Fluorescent Protein ◽  
Embryonic Stem ◽  
Green Fluorescent

Characterisation of the deleted in azoospermia like (Dazl)–green fluorescent protein mouse model generated by a two-step embryonic stem cell-based strategy to identify pluripotent and germ cells

2016 ◽  
Vol 28 (11) ◽  
pp. 1741 ◽  
Author(s):  
Priscila Ramos-Ibeas ◽  
Eva Pericuesta ◽  
Raúl Fernández-González ◽  
Alfonso Gutiérrez-Adán ◽  
Miguel Ángel Ramírez
Keyword(s):  
Stem Cells ◽  
Green Fluorescent Protein ◽  
Transgenic Mice ◽  
Germ Cells ◽  
Fluorescent Protein ◽  
Preimplantation Embryo ◽  
Embryonic Stem ◽  
Deleted In Azoospermia ◽  
Green Fluorescent

The deleted in azoospermia like (Dazl) gene is preferentially expressed in germ cells; however, recent studies indicate that it may have pluripotency-related functions. We generated Dazl–green fluorescent protein (GFP) transgenic mice and assayed the ability of Dazl-driven GFP to mark preimplantation embryo development, fetal, neonatal and adult tissues, and in vitro differentiation from embryonic stem cells (ESCs) to embryoid bodies (EBs) and to primordial germ cell (PGC)-like cells. The Dazl-GFP mice were generated by a two-step ESC-based strategy, which enabled primary and secondary screening of stably transfected clones before embryo injection. During preimplantation embryo stages, GFP was detected from the zygote to blastocyst stage. At Embryonic Day (E) 12.5, GFP was expressed in gonadal ridges and in neonatal gonads of both sexes. In adult mice, GFP expression was found during spermatogenesis from spermatogonia to elongating spermatids and in the cytoplasm of oocytes. However, GFP mRNA was also detected in other tissues harbouring multipotent cells, such as the intestine and bone marrow. Fluorescence was maintained along in vitro Dazl-GFP ESC differentiation to EBs, and in PGC-like cells. In addition to its largely known function in germ cell development, Dazl could have an additional role in pluripotency, supporting these transgenic mice as a valuable tool for the prospective identification of stem cells from several tissues.

Maintenance of Pluripotency in Human Embryonic Stem Cells Stably Over-expressing Enhanced Green Fluorescent Protein

2004 ◽  
Vol 13 (6) ◽  
pp. 636-645 ◽  
Author(s):  
Yi-Ping Liu ◽  
Oksana V. Dovzhenko ◽  
Mark A. Garthwaite ◽  
Svetlana V. Dambaeva ◽  
Maureen Durning ◽  
...  
Keyword(s):  
Stem Cells ◽  
Green Fluorescent Protein ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Enhanced Green Fluorescent Protein ◽  
Fluorescent Protein ◽  
Embryonic Stem ◽  
Green Fluorescent

scholarly journals In Vivo and In Vitro Tissue-Specific Expression of Green Fluorescent Protein Using the Cre-Lox System in Mouse Embryonic Stem Cells

Stem Cells ◽  
2005 ◽  
Vol 23 (1) ◽  
pp. 10-15 ◽  
Author(s):  
Jan Schindehütte ◽  
Hidefumi Fukumitsu ◽  
Patrick Collombat ◽  
Gundula Griesel ◽  
Christopher Brink ◽  
...  
Keyword(s):  
Stem Cells ◽  
Green Fluorescent Protein ◽  
Embryonic Stem Cells ◽  
Fluorescent Protein ◽  
Embryonic Stem ◽  
Specific Expression ◽  
Tissue Specific Expression ◽  
Green Fluorescent
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