Characterization of Membrane Steroid Binding Protein mRNA and Protein in Lens Epithelial Cells

2001 ◽  
Vol 73 (2) ◽  
pp. 213-219 ◽  
Author(s):  
Xin L Zhu ◽  
Patricia S Sexton ◽  
Richard J Cenedella
Keyword(s):  
Epithelial Cells ◽  
Binding Protein ◽  
Lens Epithelial Cells ◽  
Steroid Binding ◽  
Steroid Binding Protein

Partial purification and characterization of a membrane-associated steroid-binding protein from Pseudomonas testosteroni

1982 ◽  
Vol 60 (8) ◽  
pp. 798-803 ◽  
Author(s):  
Mike Francis ◽  
Mamoru Watanabe
Keyword(s):  
Binding Protein ◽  
Membrane Vesicles ◽  
Activity Analysis ◽  
Binding Activity ◽  
Regulatory Function ◽  
Partial Purification ◽  
Purification And Characterization ◽  
Steroid Binding ◽  
Steroid Binding Protein

A steroid-binding protein obtained from the supernatant of the final wash from the preparation of membrane vesicles was purified severalfold to near homogeneity. The protein binds C18 and C19 steroids but has the highest affinity for androstenedione (Kd = 1.6 × 10−10 M). The molecular weight is 51 000 – 58 000. Binding activity is slightly inhibited by Cu2+, Ca2+, and Mg2+ and completely inhibited by Zn2+. The protein has no detectable steroid degradative activity. Analysis of androstenedione binding revealed negative cooperativity of binding for this ligand and may indicate a regulatory function for this protein. It is postulated that this protein binds the steroid after testosterone is converted to androstenedione.

scholarly journals Mammalian expression of the human sex steroid-binding protein of plasma (SBP or SHBG) and testis (ABP) Characterization of the recombinant protein

FEBS Letters ◽  
1992 ◽  
Vol 299 (1) ◽  
pp. 23-27 ◽  
Author(s):  
Frederick S. Hagen ◽  
Cesar Arguelles ◽  
Li-ming Sui ◽  
Wei Zhang ◽  
Paul R. Seidel ◽  
...  
Keyword(s):  
Recombinant Protein ◽  
Binding Protein ◽  
Sex Steroid ◽  
Mammalian Expression ◽  
Steroid Binding ◽  
Steroid Binding Protein
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