Onchocerca volvulus Superoxide Dismutase Genes: Identification of Functional Promoters for Pre-mRNA Transcripts Which Undergo trans-Splicing

2000 ◽  
Vol 94 (3) ◽  
pp. 172-179 ◽  
Author(s):  
Wilson Tawe ◽  
Rolf D. Walter ◽  
Kimberly Henkle-Dührsen
Keyword(s):  
Superoxide Dismutase ◽  
Onchocerca Volvulus ◽  
Trans Splicing ◽  
Mrna Transcripts

scholarly journals Molecular cloning of an Onchocerca volvulus extracellular Cu-Zn superoxide dismutase.

1994 ◽  
Vol 62 (2) ◽  
pp. 713-716 ◽  
Author(s):  
E R James ◽  
D C McLean ◽  
F Perler
Keyword(s):  
Superoxide Dismutase ◽  
Molecular Cloning ◽  
Onchocerca Volvulus

scholarly journals Characterization of human immune responses to the cytosolic superoxide dismutase and glutathione S-transferase from Onchocerca volvulus

1997 ◽  
Vol 2 (8) ◽  
pp. 788-798 ◽  
Author(s):  
N. W. Brattig ◽  
K. Henkle-Duhrsen ◽  
S. Hounkpatin ◽  
E. Liebau ◽  
T. F. Kruppa ◽  
...  
Keyword(s):  
Superoxide Dismutase ◽  
Immune Responses ◽  
Onchocerca Volvulus ◽  
Glutathione S Transferase ◽  
Human Immune

scholarly journals Characterization of the manganese superoxide dismutase cDNA and gene from the human parasite Onchocerca volvulus

1995 ◽  
Vol 308 (2) ◽  
pp. 441-446 ◽  
Author(s):  
K Henkle-Dührsen ◽  
W Tawe ◽  
C Warnecke ◽  
R D Walter
Keyword(s):  
Superoxide Dismutase ◽  
Nucleotide Sequence ◽  
Adult Worm ◽  
Genomic Dna ◽  
Genomic Library ◽  
Regulatory Elements ◽  
Onchocerca Volvulus ◽  
Flanking Sequence ◽  
Degenerate Primers ◽  
Total Rna

The manganese-containing superoxide dismutase (MnSOD) is a major component of the cellular defence mechanisms against the toxic effects of the superoxide radical. Within the framework of studies on anti-oxidant enzymes and their protective role in the human parasitic nematode Onchocerca volvulus, sequences encoding the MnSOD were isolated and examined in this study. Degenerate primers were designed based upon conserved regions of MnSOD sequences from other organisms, and were used in PCR on reverse-transcribed O. volvulus total RNA and genomic DNA to identify partial cDNA and genomic DNA fragments encoding the O. volvulus MnSOD (OvMnSOD). The genomic DNA PCR product was used to screen an O. volvulus adult worm lambda unizap II cDNA library and the nucleotide sequence of the longest clone determined. The complete 5′-end of the OvMnSOD cDNA was obtained using the rapid amplification of cDNA ends (RACE) procedure with O. volvulus total RNA and was found to possess a spliced leader sequence at the 5′-terminus. The deduced primary sequence encodes a 25 kDa protein, which has the conserved residues required for enzyme activity and metal binding. The 24 N-terminal amino acids encoded by the OvMnSOD cDNA comprise a putative mitochondrial transit peptide. The OvMnSOD gene was also isolated from an O. volvulus adult worm lambda fix II genomic library, a restriction map was constructed and the nucleotide sequence determined. The OvMnSOD gene was found to possess five exons and four introns with consensus splice-site junctions. Potential regulatory elements were identified in the 5′ genomic flanking sequence. Southern-blot analysis with total worm genomic DNA indicates a single-copy gene, with a restriction pattern consistent with that of the isolated gene.

Characterization of the Onchocerca volvulus superoxide dismutase gene and mRNA processing

1993 ◽  
Vol 58 (1) ◽  
pp. 173-176 ◽  
Author(s):  
Kimberly J. Henkle ◽  
Eva Liebau ◽  
Rolf D. Walter
Keyword(s):  
Superoxide Dismutase ◽  
Mrna Processing ◽  
Onchocerca Volvulus ◽  
Superoxide Dismutase Gene

scholarly journals Correction of dystrophia myotonica type 1 pre-mRNA transcripts by artificial trans-splicing

Gene Therapy ◽  
2008 ◽  
Vol 16 (2) ◽  
pp. 211-217 ◽  
Author(s):  
H Y Chen ◽  
P Kathirvel ◽  
W C Yee ◽  
P S Lai
Keyword(s):  
Dystrophia Myotonica ◽  
Trans Splicing ◽  
Mrna Transcripts

scholarly journals Archaeal tRNA-Splicing Endonuclease as an Effector for RNA Recombination and Novel Trans-Splicing Pathways in Eukaryotes

2021 ◽  
Vol 7 (12) ◽  
pp. 1069
Author(s):  
Giuseppe D. Tocchini-Valentini ◽  
Glauco P. Tocchini-Valentini
Keyword(s):  
Schizosaccharomyces Pombe ◽  
Protein Domains ◽  
Rna Recombination ◽  
Facultative Anaerobe ◽  
Methanocaldococcus Jannaschii ◽  
Trna Splicing ◽  
Hairpin Loops ◽  
Trans Splicing ◽  
Mrna Transcripts ◽  
Ferroplasma Acidarmanus

We have characterized a homodimeric tRNA endonuclease from the euryarchaeota Ferroplasma acidarmanus (FERAC), a facultative anaerobe which can grow at temperatures ranging from 35 to 42 °C. This enzyme, contrary to the eukaryal tRNA endonucleases and the homotetrameric Methanocaldococcus jannaschii (METJA) homologs, is able to cleave minimal BHB (bulge–helix–bulge) substrates at 30 °C. The expression of this enzyme in Schizosaccharomyces pombe (SCHPO) enables the use of its properties as effectors by inserting BHB motif introns into hairpin loops normally seen in mRNA transcripts. In addition, the FERAC endonuclease can create proteins with new functionalities through the recombination of protein domains.

scholarly journals Characterization and molecular cloning of a Cu/Zn superoxide dismutase from the human parasite Onchocerca volvulus.

1991 ◽  
Vol 59 (6) ◽  
pp. 2063-2069 ◽  
Author(s):  
K J Henkle ◽  
E Liebau ◽  
S Müller ◽  
B Bergmann ◽  
R D Walter
Keyword(s):  
Superoxide Dismutase ◽  
Molecular Cloning ◽  
Onchocerca Volvulus ◽  
Human Parasite

Functional characterization and immune recognition of the extracellular superoxide dismutase from the human pathogenic parasite Onchocerca volvulus (OvEC-SOD)

Acta Tropica ◽  
2012 ◽  
Vol 124 (1) ◽  
pp. 15-26 ◽  
Author(s):  
Irene Ajonina-Ekoti ◽  
Dieudonne Ndjonka ◽  
Manchang Kingsley Tanyi ◽  
Meike Wilbertz ◽  
Abuelhassan Elshazly Younis ◽  
...  
Keyword(s):  
Superoxide Dismutase ◽  
Functional Characterization ◽  
Onchocerca Volvulus ◽  
Immune Recognition ◽  
Extracellular Superoxide Dismutase ◽  
Pathogenic Parasite

Corrigendum to “Functional characterization and immune recognition of the extracellular superoxide dismutase from the human pathogenic parasite Onchocerca volvulus (OvEC-SOD)” [Acta Trop. 124 (2012) 15–26]

Acta Tropica ◽  
2016 ◽  
Vol 161 ◽  
pp. 114 ◽  
Author(s):  
Irene Ajonina-Ekoti ◽  
Dieudonne Ndjonka ◽  
Manchang Kingsley Tanyi ◽  
Meike Wilbertz ◽  
Abuelhassan Elshazly Younis ◽  
...  
Keyword(s):  
Superoxide Dismutase ◽  
Functional Characterization ◽  
Onchocerca Volvulus ◽  
Immune Recognition ◽  
Extracellular Superoxide Dismutase ◽  
Pathogenic Parasite
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