Phylogeny of the GenusTrichodermaBased on Sequence Analysis of the Internal Transcribed Spacer Region 1 of the rDNA Cluster

1998 ◽  
Vol 24 (3) ◽  
pp. 298-309 ◽  
Author(s):  
Johanna Kindermann ◽  
Yassin El-Ayouti ◽  
Gary J. Samuels ◽  
Christian P. Kubicek
Keyword(s):  
Sequence Analysis ◽  
Internal Transcribed Spacer ◽  
Internal Transcribed Spacer Region ◽  
Rdna Cluster

scholarly journals Diversity and Aggressiveness of Rhizoctonia solani and Rhizoctonia-like Fungi on Vegetables in New York

Plant Disease ◽  
2009 ◽  
Vol 93 (6) ◽  
pp. 615-624 ◽  
Author(s):  
Mana Ohkura ◽  
George S. Abawi ◽  
Christine D. Smart ◽  
Kathie T. Hodge
Keyword(s):  
New York ◽  
Sequence Analysis ◽  
Rhizoctonia Solani ◽  
Internal Transcribed Spacer ◽  
Anastomosis Group ◽  
Internal Transcribed Spacer Region ◽  
Snap Bean ◽  
First Report ◽  
Pathogenicity Tests ◽  
Waitea Circinata

Vegetable growers in New York, especially those growing table beets, have recently observed that the corn rotation is no longer effective in suppressing diseases caused by Rhizoctonia solani and Rhizoctonia-like fungi. To investigate this problem, 68 isolates of Rhizoctonia solani and Rhizoctonia-like fungi infecting vegetables in New York were isolated, characterized, and their pathogenicity on corn determined. Sequence analysis of the rDNA internal transcribed spacer region inferred 26 isolates to belong to R. solani anastomosis group (AG) 2-2 and 19 isolates to belong to AG 4. Remaining isolates belonged to AG 1, AG 2-1, AG 5, AG 11, Ceratobasidium AG (CAG) 2, CAG 6, and Waitea circinata var. zeae. This is a first report of AG 11 and W. circinata var. zeae recovered from naturally infected vegetables in New York. Pathogenicity tests on corn showed that the majority of isolates are pathogenic on corn, and isolates belonging to AG 2-2, AG 5, and AG 11 exhibited high aggressiveness. These results suggest that certain strains of R. solani and Rhizoctonia-like fungi infecting vegetables in New York have acquired the ability to infect corn. In addition, snap bean was inoculated with seven isolates exhibiting low to high aggressiveness on corn, and a correlation between aggressiveness on corn and snap bean was observed.

scholarly journals Occurrence of Togninia minima Perithecia in Esca-Affected Vineyards in California

Plant Disease ◽  
2005 ◽  
Vol 89 (8) ◽  
pp. 867-871 ◽  
Author(s):  
S. Rooney-Latham ◽  
A. Eskalen ◽  
W. D. Gubler
Keyword(s):  
Sequence Analysis ◽  
Internal Transcribed Spacer ◽  
Vascular Tissue ◽  
Agar Medium ◽  
Nuclear Ribosomal Dna ◽  
Additional Source ◽  
Internal Transcribed Spacer Region ◽  
Molecular Sequence ◽  
Molecular Sequence Analysis

Togninia minima is an important pathogen causing esca and grapevine declines worldwide. Although perithecia of T. minima have been produced in the laboratory, their presence in diseased vineyards has not been shown. In our study, perithecia of T. minima were found on grapevines in the field in five California counties. Perithecia were clustered on dead vascular tissue in deep cracks along trunks and cordons or on the surfaces of decayed pruning wounds. Field-collected perithecia were characteristic of T. minima perithecia previously produced in vitro and molecular sequence analysis of the internal transcribed spacer region of the nuclear ribosomal DNA additionally confirmed their identity. Ascospores from perithecia germinated on agar medium and formed colonies typical of T. minima. This is the first report of T. minima perithecia in diseased vineyards and suggests ascospores as an additional source of inoculum for new grapevine infections.

scholarly journals Phoma glomerata as a Mycoparasite of Powdery Mildew

2000 ◽  
Vol 66 (1) ◽  
pp. 425-427 ◽  
Author(s):  
Raymond F. Sullivan ◽  
James F. White
Keyword(s):  
Life Cycle ◽  
Sequence Analysis ◽  
Powdery Mildew ◽  
Ribosomal Dna ◽  
Internal Transcribed Spacer ◽  
Internal Transcribed Spacer Region

ABSTRACT Ampelomyces and Phoma species are frequently confused with each other. Isolates previously attributed to the genus Ampelomyces were shown to be Phomaisolates through studies of their morphology and life cycle and ribosomal DNA internal transcribed spacer region 1 sequence analysis.Phoma glomerata can colonize and suppress development of powdery mildew on oak and may have utility as a mycoparasitic agent.

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