scholarly journals Neuroendocrine Diffuse System of the Respiratory Tract of Rana temporaria: An Immunocytochemical Study

1995 ◽  
Vol 100 (2) ◽  
pp. 145-161 ◽  
Author(s):  
M.E. Bodegas ◽  
L.M. Montuenga ◽  
P. Sesma
Keyword(s):  
Respiratory Tract ◽  
Rana Temporaria ◽  
Immunocytochemical Study

scholarly journals Neuronal nitric oxide synthase immunoreactivity in the respiratory tract of the frog, Rana temporaria

1995 ◽  
Vol 27 (10) ◽  
pp. 812-818
Author(s):  
M. E. Bodegas ◽  
A. C. Villaro ◽  
L. M. Montuenga ◽  
S. Moncada ◽  
V. Riveros-Moreno ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Nitric Oxide Synthase ◽  
Respiratory Tract ◽  
Rana Temporaria ◽  
Neuronal Nitric Oxide Synthase ◽  
Frog Rana Temporaria ◽  
Oxide Synthase

scholarly journals Neuronal nitric oxide synthase immunoreactivity in the respiratory tract of the frog,Rana temporaria

1995 ◽  
Vol 27 (10) ◽  
pp. 812-818 ◽  
Author(s):  
M. E. Bodegas ◽  
A. C. Villaro ◽  
L. M. Montuenga ◽  
S. Moncada ◽  
V. Riveros-Moreno ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Nitric Oxide Synthase ◽  
Respiratory Tract ◽  
Rana Temporaria ◽  
Neuronal Nitric Oxide Synthase ◽  
Frog Rana Temporaria ◽  
Oxide Synthase

New Ultrastructural Observations on Injury and Regeneration of Cilia in Mammalian Conducting Airway Epithelium

1981 ◽  
Vol 39 ◽  
pp. 624-625
Author(s):  
J.L. Carson ◽  
A.M. Collier
Keyword(s):  
Respiratory Tract ◽  
Airway Epithelium ◽  
Defense Mechanisms ◽  
Normal Growth ◽  
Fetal Lung ◽  
Secretory Cells ◽  
Airway Epithelial ◽  
Ciliated Cells ◽  
Conducting Airway ◽  
Conducting Airways

The ciliated cells lining the conducting airways of mammals are integral to the defense mechanisms of the respiratory tract, functioning in coordination with secretory cells in the removal of inhaled and cellular debris. The effects of various infectious and toxic agents on the structure and function of airway epithelial cell cilia have been studied in our laboratory, both of which have been shown to affect ciliary ultrastructure.These observations have led to questions about ciliary regeneration as well as the possible induction of ciliogenesis in response to cellular injury. Classical models of ciliogenesis in the conducting airway epithelium of the mammalian respiratory tract have been based primarily on observations of the developing fetal lung. These observations provide a plausible explanation for the embryological generation of ciliary beds lining the conducting airways but do little to account for subsequent differentiation of ciliated cells and ciliogenesis during normal growth and development.

An immunocytochemical study of maternal immunoglobulin localization in the suckling pig intestine

1990 ◽  
Vol 48 (3) ◽  
pp. 922-923
Author(s):  
László G. Kömüves ◽  
Donna S. Turner ◽  
Kathy S. McKee ◽  
Buford L. Nichols ◽  
Julian P. Heath
Keyword(s):  
Sodium Ethoxide ◽  
Intracellular Localization ◽  
Protein A ◽  
Tween 20 ◽  
Intestinal Epithelial ◽  
Immunocytochemical Study ◽  
Fish Skin ◽  
Newborn Piglets ◽  
Rabbit Igg ◽  
Fish Skin Gelatin

In this study we used colloidal gold probes to detect the intracellular localization of colostral immunoglobulins in intestinal epithelial cells of newborn piglets.Tissues were obtained from non-suckled newborn and suckled piglets aged between 1 hour to 1 month. Samples were fixed in 2.5 % glutaraldehyde, osmicated and embedded into Spurr’s resin. Thin (80 nm) sections were etched with 5% sodium ethoxide for 5 min, washed and treated with 4 % sodium-m-periodate in distilled water for 30 min. The sections were then first incubated with blocking buffer (2 % BSA, 0.25 % fish skin gelatin, 0.5 % Tween 20 in 10 mM Trizma buffer, pH=7.4 containing 500 mM NaCl) for 30 min followed by the immunoreagents diluted in the same buffer, 1 hr each. For the detection of pig immunoglobulins a rabbit anti-pig IgG antiserum was used followed by goat anti-rabbit IgG-Au10 or protein A-Au15 probes.

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