Testing Suitability of Brush Border Membrane Vesicles Prepared from Whole Larvae from Small Insects for Binding Studies with Bacillus thuringiensis CrylA(b) Crystal Protein

1995 ◽  
Vol 65 (3) ◽  
pp. 318-320 ◽  
Author(s):  
Baltasar Escriche ◽  
Francisco J. Silva ◽  
Juan Ferré
Keyword(s):  
Bacillus Thuringiensis ◽  
Brush Border ◽  
Brush Border Membrane ◽  
Membrane Vesicles ◽  
Brush Border Membrane Vesicles ◽  
Crystal Protein ◽  
Binding Studies

scholarly journals Pore-forming properties of the Bacillus thuringiensis toxin Cry9Ca in Manduca sexta brush border membrane vesicles

2010 ◽  
Vol 1798 (6) ◽  
pp. 1111-1118 ◽  
Author(s):  
Jean-Frédéric Brunet ◽  
Vincent Vachon ◽  
Marc Juteau ◽  
Jeroen Van Rie ◽  
Geneviève Larouche ◽  
...  
Keyword(s):  
Bacillus Thuringiensis ◽  
Manduca Sexta ◽  
Brush Border ◽  
Brush Border Membrane ◽  
Membrane Vesicles ◽  
Brush Border Membrane Vesicles ◽  
Bacillus Thuringiensis Toxin

scholarly journals Changes in Permeability of Brush Border Membrane Vesicles fromSpodoptera littoralis Midgut Induced by Insecticidal Crystal Proteins from Bacillus thuringiensis

1998 ◽  
Vol 64 (4) ◽  
pp. 1563-1565 ◽  
Author(s):  
B. Escriche ◽  
N. De Decker ◽  
J. Van Rie ◽  
S. Jansens ◽  
E. Van Kerkhove
Keyword(s):  
Bacillus Thuringiensis ◽  
Brush Border ◽  
Brush Border Membrane ◽  
Membrane Vesicles ◽  
Brush Border Membrane Vesicles ◽  
Osmotic Swelling ◽  
Midgut Cell ◽  
Crystal Proteins ◽  
Light Scattering Technique ◽  
Insecticidal Crystal Proteins

ABSTRACT Bacillus thuringiensis insecticidal crystal proteins (ICPs) are thought to induce pore formation in midgut cell membranes of susceptible insects. Cry1Ca, which is significantly active inSpodoptera littoralis, made brush border membrane vesicles permeable to KCl (osmotic swelling was monitored by the light scattering technique); the marginally active ICPs Cry1Aa, Cry1Ab, and Cry1Ac did not.

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