Alkaline Phosphatase-Strep tag fusion protein binding to streptavidin: resonant mirror studies 1 1Edited by A. R. Fersht

1997 ◽  
Vol 266 (3) ◽  
pp. 621-632 ◽  
Author(s):  
M Hengsakul ◽  
A.E.G Cass
Keyword(s):  
Alkaline Phosphatase ◽  
Fusion Protein ◽  
Protein Binding

scholarly journals Placental Lactogen-I (PL-I) Target Tissues Identified with an Alkaline Phosphatase-PL-I Fusion Protein

1998 ◽  
Vol 46 (6) ◽  
pp. 737-743 ◽  
Author(s):  
Heiner Müller ◽  
Guoli Dai ◽  
Michael J. Soares
Keyword(s):  
Alkaline Phosphatase ◽  
Fusion Protein ◽  
Colorimetric Assay ◽  
Biological Activities ◽  
Placental Lactogen ◽  
Kidney Cells ◽  
Fetal Kidney ◽  
Transfected Cells ◽  
Tissue Sections ◽  
Labeling System

The rat placenta expresses a family of genes related to prolactin (PRL). Target tissues and physiological roles for many members of the PRL family have yet to be determined. In this investigation we evaluated the use of an alkaline phosphatase (AP) tag for monitoring the behavior of a prototypical member of the PRL family, placental lactogen-I (PL-I). A probe was generated consisting of a fusion protein of human placental AP and rat PL-I (AP-PL-I). The AP-PL-I construct was stably expressed in 293 human fetal kidney cells, as was the unmodified AP vector that served as a control. AP activity was monitored with a colorimetric assay in conditioned medium from transfected cells. Immunoreactivity and PRL-like biological activities of the AP-PL-I fusion protein were demonstrated by immunoblotting and the Nb2 lymphoma cell proliferation assay, respectively. AP-PL-I specifically bound to tissue sections known to express the PRL receptor, including the ovary, liver, and choroid plexus. Binding of AP-PL-I to tissues was specific and could be competed with ovine PRL. The results indicate that AP is an effective tag for monitoring the behavior of PL-I and suggest that this labeling system may also be useful for monitoring the actions of other members of the PRL family.

scholarly journals Development of a one-step immunoassay for triazophos using camel single-domain antibody–alkaline phosphatase fusion protein

2019 ◽  
Vol 411 (6) ◽  
pp. 1287-1295 ◽  
Author(s):  
Kai Wang ◽  
Zhiping Liu ◽  
Guochun Ding ◽  
Ji Li ◽  
Natalia Vasylieva ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Fusion Protein ◽  
Single Domain ◽  
Single Domain Antibody ◽  
Domain Antibody ◽  
One Step

scholarly journals One-Step Immunoassay for Tetrabromobisphenol A Using a Camelid Single Domain Antibody–Alkaline Phosphatase Fusion Protein

2015 ◽  
Vol 87 (9) ◽  
pp. 4741-4748 ◽  
Author(s):  
Jia Wang ◽  
Zuzana Majkova ◽  
Candace R. S. Bever ◽  
Jun Yang ◽  
Shirley J. Gee ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Fusion Protein ◽  
Single Domain ◽  
Tetrabromobisphenol A ◽  
Single Domain Antibody ◽  
Domain Antibody ◽  
One Step

Construction of Single Chain Variable Fragment (ScFv) and BiscFv-Alkaline Phosphatase Fusion Protein for Detection ofBacillusAnthracis

2006 ◽  
Vol 78 (4) ◽  
pp. 997-1004 ◽  
Author(s):  
Shi-Hua Wang ◽  
Ji-Bin Zhang ◽  
Zhi-Ping Zhang ◽  
Ya-Feng Zhou ◽  
Rui-Fu Yang ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Fusion Protein ◽  
Single Chain Variable Fragment ◽  
Single Chain
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