Mammalian RNA polymerase I exists as a holoenzyme with associated basal transcription factors 1 1Edited by M. Yahiv

1998 ◽  
Vol 275 (1) ◽  
pp. 43-53 ◽  
Author(s):  
Peter Seither ◽  
Sebastian Iben ◽  
Ingrid Grummt
Keyword(s):  
Transcription Factors ◽  
Rna Polymerase ◽  
Rna Polymerase I ◽  
Basal Transcription ◽  
Basal Transcription Factors ◽  
Polymerase I

scholarly journals Analysis of the phosphorylation, DNA-binding and dimerization properties of the RNA polymerase I transcription factors UBF1 and UBF2

1992 ◽  
Vol 20 (6) ◽  
pp. 1301-1308 ◽  
Author(s):  
Daniel J.O. Mahony ◽  
S. David Smith ◽  
WenQin Xie ◽  
Lawrence I. Rothblum
Keyword(s):  
Transcription Factors ◽  
Dna Binding ◽  
Rna Polymerase ◽  
Rna Polymerase I ◽  
Polymerase I

scholarly journals Interaction of RNA polymerase I transcription factors with a promoter in the nontranscribed spacer of rat ribosomal DNA

1990 ◽  
Vol 18 (7) ◽  
pp. 1677-1718 ◽  
Author(s):  
S.David Smith ◽  
Emmanuel Oriahi ◽  
Hsin-Fang Yang-Yen ◽  
WenQin Xie ◽  
Catherine Chen ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Rna Polymerase ◽  
Ribosomal Dna ◽  
Rna Polymerase I ◽  
Nontranscribed Spacer ◽  
Polymerase I

scholarly journals In Vivo Binding and Hierarchy of Assembly of the Yeast RNA Polymerase I Transcription Factors

2001 ◽  
Vol 12 (3) ◽  
pp. 753-760 ◽  
Author(s):  
Licia Bordi ◽  
Francesco Cioci ◽  
Giorgio Camilloni
Keyword(s):  
Transcription Factors ◽  
Rna Polymerase ◽  
Rna Polymerase I ◽  
35S Promoter ◽  
Core Element ◽  
The Core ◽  
Polymerase I ◽  
Functional Rna

Transcription by RNA polymerase I in Saccharomyces cerevisiae requires a series of transcription factors that have been genetically and biochemically identified. In particular, the core factor (CF) and the upstream activation factor (UAF) have been shown in vitro to bind the core element and the upstream promoter element, respectively. We have analyzed in vivo the DNAse I footprinting of the 35S promoter in wild-type and mutant strains lacking one specific transcription factor at the time. In this way we were able to unambiguously attribute the protections by the CF and the UAF to their respective putative binding sites. In addition, we have found that in vivo a binding hierarchy exists, the UAF being necessary for CF binding. Because the CF footprinting is lost in mutants lacking a functional RNA polymerase I, we also conclude that the final step of preinitiation-complex assembly affects binding of the CF, stabilizing its contact with DNA. Thus, in vivo, the CF is recruited to the core element by the UAF and stabilized on DNA by the presence of a functional RNA polymerase I.

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